Fermentation preparation method of monascus pigment

A technology of monascus pigment and monascus fungus, which is applied in the field of fermentation and preparation of monascus pigment with high color price and high biological safety, can solve the problems of long production time and low efficiency, achieve increased production, simple operation and practicality strong effect

Inactive Publication Date: 2017-02-15
FUZHOU UNIVERSITY
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the production time of traditional monascus pigment is long, the efficiency

Method used

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  • Fermentation preparation method of monascus pigment
  • Fermentation preparation method of monascus pigment

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] Example 1 Liquid Fermentation

[0022] (1) Take the slant of Monascus purple 183-3 strain, pick the spores with the inoculation loop after burning and sterilize, streak on the PDA plate, culture at 30°C for 3-4 days, and pick the fast-growing and large colony for subculture , to obtain a strong growth Monascus strain;

[0023] (2) Use a puncher to punch out 2 bacteria cakes (6mm) on the Monascus plate, inoculate them into PD liquid medium (the medium is divided into 250mL Erlenmeyer flasks, and the liquid volume is 50mL / bottle), and place In a reciprocating shaker, culture at a constant temperature of 32°C and 120r / min for 3 days to obtain Monascus seed liquid;

[0024] (3) Divide the liquid fermentation medium into 250mL Erlenmeyer flasks with a liquid volume of 50mL / bottle, then add 5mL of Monascus seed liquid, and culture at 32°C and 180r / min for 12 days to obtain fermentation broth.

[0025] The preparation method of the PD liquid medium used is as follows: peel a...

Embodiment 2

[0027] Example 2 The removal of citrinin

[0028] (1) Take 10 mL of fermentation broth, add 90 mL of ethanol solution with a volume concentration of 75%, oscillate and mix well, place in the dark at room temperature for 20 min, take it out and centrifuge at 4000 r / min for 8 min, and evaporate the obtained supernatant in a rotary evaporator at 55 °C. dry, then add 5 mL of ethanol solution with a volume concentration of 75% to dissolve to obtain a concentrated solution;

[0029] (2) Soak 10g, 80-120 mesh silica gel for column chromatography with ethyl acetate and then wet-pack the column. The diameter of the column is 10mm, and the height of the column is 10cm; For the eluent to elute, the flow rate of the eluent is controlled at 1-2 drops / second, and the collection is carried out according to the interval bands of different colors on the silica gel column;

[0030] (3) Evaporate the eluate collected in the red zone to dryness at 55°C in a rotary evaporator, add 3 mL of ethanol...

Embodiment 3

[0031] Embodiment 3 HPLC detection method

[0032] Using a high performance liquid chromatography system with a fluorescence detector

[0033] Chromatographic column: HITACHI LaChromUltra C18 (150mm×4.6mm, particle size 5µm);

[0034] Mobile phase: acetonitrile: ultrapure water (adjust pH to 2.5 with phosphoric acid) = 35:65;

[0035] Detector: fluorescence detector, excitation wavelength 331nm, emission wavelength 500nm;

[0036] Injection volume: 20µL;

[0037] Flow rate: 1 mL / min;

[0038] Column temperature: 28°C.

[0039] Experimental results: figure 1 , 2 are the high-performance liquid chromatograms of the detected fermentation broth and sample solution, respectively. It can be seen from the figure that the content of citrinin (t=12.989) in the fermentation broth is relatively high, but the sample solution after silica gel column chromatography hardly contains citrinin.

[0040] After calculation, the content of citrinin in the fermentation broth was 39.09 mg / L,...

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Abstract

The invention discloses a fermentation preparation method of monascus pigment with high color value and high biological safety. The fermentation preparation method is characterized in that a liquid-state fermentation culture medium is prepared by adopting soluble starch, sulfate and the like as raw materials, and a silica gel column chromatography is combined on the basis of liquid-state fermentation to remove citrinin in monascus-pigment fermented liquid. The fermentation preparation method disclosed by the invention has the advantages that the yield of the monascus pigment can be increased, and meanwhile the citrinin can be effectively removed, so that foundation is laid for wider application of the monascus pigment in the food industry.

Description

technical field [0001] The invention belongs to the field of biological fermentation preparation, and in particular relates to a fermentation preparation method of monascus pigment with high color value and high biological safety. Background technique [0002] Monascus pigment is a secondary metabolite produced by the fermentation of filamentous fungi of the genus Monascus. It is often selected as a high-quality natural food coloring due to its stable properties, good coloring properties, and non-toxicity. However, the production time of traditional monascus pigment is long and the efficiency is low, and the production process is often accompanied by the formation of citrinin. However, citrinin has strong nephrotoxicity. The national standard (GB / T5009.222-2008) limits the content of citrinin in liquid and solid samples of red yeast rice: liquid <50μg / L; solid <1mg / kg . Contents of the invention [0003] The purpose of the present invention is to provide a method f...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12R1/66
CPCC12P1/02C12N1/145C12R2001/66
Inventor 林娟陈景智李亮苏冰梅叶秀云
Owner FUZHOU UNIVERSITY
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