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Preparation method of micafungin precursor

A technology of micafungin and precursor, which is applied in the field of preparation of micafungin precursor, can solve problems such as being not suitable for industrialized large-scale production, tedious operation steps of micafungin precursor, complicated preparation process and the like , to achieve the effect of stable method, good reproducibility and simple operation procedure

Inactive Publication Date: 2017-02-15
WUXI FORTUNE PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Therefore, there are also cumbersome operating steps for preparing the micafungin precursor, complicated preparation process, and the need to use chemical reagents such as ketones that are harmful to the human body and the environment, so it is not suitable for large-scale industrial production

Method used

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  • Preparation method of micafungin precursor

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Effect test

Embodiment 1

[0029] The preparation method of the micafungin precursor comprises the following steps.

[0030] Step (1), take a small amount of micafungin precursor, ie FR179642 (ie WF11899A) producing spore suspension, inoculate it on the seed medium for cultivation. The components in the seed medium are (unit: g / L): glucose 15, glycerol 15, corn steep liquor powder 30, yeast powder 15, tryptone 15, sodium chloride 3, potassium dihydrogen phosphate 3, calcium carbonate 3 , and the balance is water. Incubate at 24°C for 2 days.

[0031] Step (2), inoculating the seed medium into the fermenter and adding the fermentation medium for fermentation. The components in the fermentation medium are (unit: g / L): glucose 15, sorbitol 90, corn steep liquor powder 10, soybean cake powder 30, triammonium citrate 4, ferrous sulfate heptahydrate 0.4, ammonium sulfate 4 , magnesium sulfate heptahydrate 3, calcium carbonate 5, dipotassium hydrogen phosphate 3, and the balance is water. Cultured at 28°C ...

Embodiment 2

[0038] The preparation method of the micafungin precursor comprises the following steps.

[0039] Step (1), take a small amount of micafungin precursor, ie FR179642 (ie WF11899A) producing spore suspension, and inoculate it on the seed medium for cultivation. The components in the seed medium components are (unit: g / L): 5 glucose, 5 glycerol, 10 corn steep liquor dry powder, 5 yeast powder, 5 tryptone, 1 sodium chloride, 1 potassium dihydrogen phosphate, carbonic acid Calcium 1, the balance is water. Cultured at 28°C for 4 days.

[0040]Step (2), inoculating the seed medium into the fermenter and adding the fermentation medium for fermentation. The components in the fermentation medium are (unit: g / L): glucose 5, sorbitol 70, corn steep liquor powder 6, soybean cake powder 20, triammonium citrate 2, ferrous sulfate heptahydrate 0.2, ammonium sulfate 2 , 1 magnesium sulfate heptahydrate, 3 calcium carbonate, 1 dipotassium hydrogen phosphate, and the balance is water. Cultur...

Embodiment 3

[0047] The preparation method of the micafungin precursor comprises the following steps.

[0048] Step (1), take a small amount of micafungin precursor, ie FR179642 (ie WF11899A) producing spore suspension, and inoculate it on the seed medium for cultivation. The components in the seed medium are (unit: g / L): glucose 10, glycerin 10, corn steep liquor powder 20, yeast powder 10, tryptone 10, sodium chloride 2, potassium dihydrogen phosphate 2, calcium carbonate 2 , and the balance is water. Cultured at 26°C for 3 days.

[0049] Step (2), inoculating the seed medium into the fermenter and adding the fermentation medium for fermentation. The components in the fermentation medium are (unit: g / L): glucose 10, sorbitol 80, corn steep liquor powder 8, soybean cake powder 25, triammonium citrate 3, ferrous sulfate heptahydrate 0.3, ammonium sulfate 3 , magnesium sulfate heptahydrate 2, calcium carbonate 4, dipotassium hydrogen phosphate 2, and the balance is water. Cultured at 26...

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Abstract

The invention provides a preparation method of micafungin precursor. The preparation method includes steps of producing strain spore suspension liquid through a few amounts of micafungin precursor and cultivating, and adding oxygen carrier in the fermentation liquid during the fermenting process, thus the dissolved oxygen concentration in a fermentation cylinder is improved, and 20-40% of output of the micafungin precursor is improved; performing the acidification and wall breaking treatment on the fermentation liquid, absorbing polar solvent by macroporous resin, analyzing and obtaining desorbed solution of micafungin precursor; crystalizing the desorbed solution and obtaining micafungin precursor powder. The invention can be used for compounding the micafungin precursor, and is simple in operation procedure, stable in method, good in reproducibility, and very suitable for industrial production; meanwhile, the method avoids using acetone and other poisonous solvent and lightens hazard to environment and human body.

Description

technical field [0001] The invention relates to the technical field of biopharmaceuticals, in particular to a preparation method of a micafungin precursor. Background technique [0002] In recent years, with the extensive application of broad-spectrum antibacterial drugs and immunosuppressants, tumor chemotherapy, organ transplantation, and the spread of AIDS, the number of people with suppressed immune systems has increased, and the incidence of deep fungal infections has begun to show a clear upward trend. , and with the application of antifungal drugs, the drug resistance of fungi is getting stronger and stronger, making the application of antifungal drugs have a trend of rapid increase. Therefore, anti-deep fungal infection drugs have become one of the research hotspots of anti-infective drugs and have attracted people's attention day by day. [0003] Echinocandin is a new type of antifungal drug, which has a significantly different mechanism of action from the current ...

Claims

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Application Information

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IPC IPC(8): C12P21/04C07K1/16
CPCC07K7/56
Inventor 游云龙付静曹峥杨亮赵永俊蔡云洁金丹甜
Owner WUXI FORTUNE PHARMA
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