Preparation method of micafungin precursor
A technology of micafungin and precursor, which is applied in the field of preparation of micafungin precursor, can solve problems such as being not suitable for industrialized large-scale production, tedious operation steps of micafungin precursor, complicated preparation process and the like , to achieve the effect of stable method, good reproducibility and simple operation procedure
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Embodiment 1
[0029] The preparation method of the micafungin precursor comprises the following steps.
[0030] Step (1), take a small amount of micafungin precursor, ie FR179642 (ie WF11899A) producing spore suspension, inoculate it on the seed medium for cultivation. The components in the seed medium are (unit: g / L): glucose 15, glycerol 15, corn steep liquor powder 30, yeast powder 15, tryptone 15, sodium chloride 3, potassium dihydrogen phosphate 3, calcium carbonate 3 , and the balance is water. Incubate at 24°C for 2 days.
[0031] Step (2), inoculating the seed medium into the fermenter and adding the fermentation medium for fermentation. The components in the fermentation medium are (unit: g / L): glucose 15, sorbitol 90, corn steep liquor powder 10, soybean cake powder 30, triammonium citrate 4, ferrous sulfate heptahydrate 0.4, ammonium sulfate 4 , magnesium sulfate heptahydrate 3, calcium carbonate 5, dipotassium hydrogen phosphate 3, and the balance is water. Cultured at 28°C ...
Embodiment 2
[0038] The preparation method of the micafungin precursor comprises the following steps.
[0039] Step (1), take a small amount of micafungin precursor, ie FR179642 (ie WF11899A) producing spore suspension, and inoculate it on the seed medium for cultivation. The components in the seed medium components are (unit: g / L): 5 glucose, 5 glycerol, 10 corn steep liquor dry powder, 5 yeast powder, 5 tryptone, 1 sodium chloride, 1 potassium dihydrogen phosphate, carbonic acid Calcium 1, the balance is water. Cultured at 28°C for 4 days.
[0040]Step (2), inoculating the seed medium into the fermenter and adding the fermentation medium for fermentation. The components in the fermentation medium are (unit: g / L): glucose 5, sorbitol 70, corn steep liquor powder 6, soybean cake powder 20, triammonium citrate 2, ferrous sulfate heptahydrate 0.2, ammonium sulfate 2 , 1 magnesium sulfate heptahydrate, 3 calcium carbonate, 1 dipotassium hydrogen phosphate, and the balance is water. Cultur...
Embodiment 3
[0047] The preparation method of the micafungin precursor comprises the following steps.
[0048] Step (1), take a small amount of micafungin precursor, ie FR179642 (ie WF11899A) producing spore suspension, and inoculate it on the seed medium for cultivation. The components in the seed medium are (unit: g / L): glucose 10, glycerin 10, corn steep liquor powder 20, yeast powder 10, tryptone 10, sodium chloride 2, potassium dihydrogen phosphate 2, calcium carbonate 2 , and the balance is water. Cultured at 26°C for 3 days.
[0049] Step (2), inoculating the seed medium into the fermenter and adding the fermentation medium for fermentation. The components in the fermentation medium are (unit: g / L): glucose 10, sorbitol 80, corn steep liquor powder 8, soybean cake powder 25, triammonium citrate 3, ferrous sulfate heptahydrate 0.3, ammonium sulfate 3 , magnesium sulfate heptahydrate 2, calcium carbonate 4, dipotassium hydrogen phosphate 2, and the balance is water. Cultured at 26...
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