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An electrochemical biosensor for detecting hydrogen peroxide and its preparation and application

A biosensor and hydrogen peroxide technology, applied in the direction of material electrochemical variables, scientific instruments, and material analysis through electromagnetic means, can solve the problems of weak signal corresponding strength, small specific surface area of ​​electrodes, and poor conductivity, etc., to achieve The effect of enhanced signal strength and sensitivity, good conductivity, and accelerated transmission rate

Active Publication Date: 2018-09-14
广东省汕头市质量计量监督检测所
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The object of the present invention is to provide an electrochemical biosensor for detecting hydrogen peroxide and its preparation and application to solve the problem that the electrode specific surface area of ​​the current enzyme biosensor is too small, the conductivity is not good, the corresponding signal intensity is too weak, and the sensitivity is too low. Problems such as too low

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  • An electrochemical biosensor for detecting hydrogen peroxide and its preparation and application
  • An electrochemical biosensor for detecting hydrogen peroxide and its preparation and application
  • An electrochemical biosensor for detecting hydrogen peroxide and its preparation and application

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Embodiment 1

[0037] A method for preparing an electrochemical biosensor for detecting hydrogen peroxide, comprising the following steps:

[0038] (1) Glassy carbon electrode pretreatment: Glassy carbon electrode is treated with 0.05μm α-A1 2 o 3 After polishing, the polishing powder was rinsed with deionized water, and ultrasonicated in deionized water and ethanol for 10 min, respectively. A three-electrode system is used for detection, the working electrode is a glassy carbon electrode, the counter electrode is a platinum wire electrode, and the reference electrode is an Ag / AgCl electrode. In 0.5 M sulfuric acid, set the voltage at -0.2 to 1.6V to cycle the glassy carbon electrode. Voltammetric scanning, after the detection, rinse the electrode with deionized water, dry the surface of the electrode, and set aside.

[0039] (2) Au / N-C / SiO 2 / Preparation of 3D-GONs modified glassy carbon electrodes:

[0040] a. Synthesis of three-dimensional graphene: graphene oxide was configured to a...

Embodiment 2

[0054] A method for preparing an electrochemical biosensor for detecting hydrogen peroxide, comprising the following steps:

[0055] (1) Glassy carbon electrode pretreatment: Glassy carbon electrode is treated with 0.05μm α-A1 2 o 3 After polishing, the polishing powder was rinsed with deionized water, and ultrasonicated in deionized water and ethanol for 10 min, respectively. A three-electrode system is used for detection, the working electrode is a glassy carbon electrode, the counter electrode is a platinum wire electrode, and the reference electrode is an Ag / AgCl electrode. In 0.5 M sulfuric acid, set the voltage at -0.2 to 1.6V to cycle the glassy carbon electrode. Voltammetric scanning, after the detection, rinse the electrode with deionized water, dry the surface of the electrode, and set aside.

[0056] (2) Au / N-C / SiO 2 / Preparation of 3D-GONs modified glassy carbon electrodes:

[0057] a. Synthesis of three-dimensional graphene: graphene oxide was configured to a...

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Abstract

The present invention relates to an electrochemical biosensor for detecting hydrogen peroxide, a preparation method and an application thereof. The preparation method comprises the steps of (1) synthesizing the silica-three-dimensional graphene composite material; (2) doping nitrogen and carbon into the synthesized silica-three-dimensional graphene composite material; (3) pretreating a glassy carbon electrode; (4) preparing the glassy carbon electrode modified by the nitrogen-carbon-doped silica-three-dimensional graphene composite material through the dispensing method; (5) putting the glassy carbon electrode into a chloroauric acid solution to prepare gold nanoparticle-nitrogen-carbon-doped silica-three-dimensional graphene glassy carbon electrode through chronoamperometry; (6) dipping the glassy carbon electrode into a double-sulfhydryl-modified GDNA solution and cultivating for 2-4 hours; (7) dipping the composite electrode into a hemin solution for 10-14 hours. The electrochemical biosensor is good in stability and reproducibility, high in specificity and sensitivity, fast and simple and convenient to operate. The electrochemical biosensor can be used for detecting the content of hydrogen peroxide in biological samples, cosmetics and the like.

Description

technical field [0001] The invention relates to the field of analytical chemistry and electrochemical sensor analysis, in particular to a sensor based on Au / N-C / SiO 2 Electrochemical biosensor for detecting hydrogen peroxide of / 3D-GONs composite material, preparation method and application. Background technique [0002] Hydrogen peroxide is a component that is ubiquitous in nature. For example: produced in the liver of organisms; present in polluted air or rainwater; some lactic acid bacteria used in dairy products such as yogurt can also produce hydrogen peroxide. In the food industry, hydrogen peroxide is mainly used for disinfection, sterilization, bleaching, decolorization, etc., and is also used as a production and processing aid. As an important bleaching agent and oxidizing agent, hydrogen peroxide is also widely used in hair dyeing and hair perm cosmetics. Due to its strong oxidizing properties, excessive use will cause serious damage to the skin and hair. [000...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N27/48G01N27/327G01N27/30
CPCG01N27/308G01N27/3278G01N27/48
Inventor 杨建英郑增尧徐宁林旋琴刘志宏陈泽平黄让明方铭岳
Owner 广东省汕头市质量计量监督检测所