Activated fluorescent latex microsphere for procalcitonin fluorescent immunochromatographic test card

A fluorescent latex microsphere and fluorescent immunochromatography technology, which is applied in measurement devices, analytical materials, biological tests, etc., can solve problems such as reduced detection sensitivity, uneven dispersion, and chromatography failure, and achieve stable detection results. Accurate, easy-to-use results

Active Publication Date: 2017-02-15
BEIJING ELCOTEQ BIO TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The technical problem to be solved by the present invention is: after drying, the fluorescent latex microspheres of the procalcitonin (PCT) fluorescent immunochromatography detection card tend to agglomerate and disperse unevenly, resulting in chromatography failure or lower detection sensitivity

Method used

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  • Activated fluorescent latex microsphere for procalcitonin fluorescent immunochromatographic test card
  • Activated fluorescent latex microsphere for procalcitonin fluorescent immunochromatographic test card
  • Activated fluorescent latex microsphere for procalcitonin fluorescent immunochromatographic test card

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0047] , the amino acid type surfactant is sodium lauroyl glutamate; the stearate is sodium stearate; the polyethylene glycol fatty acid ester is polyethylene glycol monolaurate.

[0048] 1) Dissolve the surfactant (4 mg sodium lauroyl glutamate, 4 mg polyethylene glycol monolaurate, 2 mg sodium stearate) in 0.5M carbonic acid buffer (H value to 9.6) at pH 9.6 , then add in 0.1ml of DMF, 0.1ml of DMF, add N,N'-dicyclohexylcarbodiimide (DCC) 1mg and 0.6mg N-hydroxysuccinimide (NHS) and stir at room temperature for 3 Hour.

[0049] 2) Adjust the pH value of 1ml of fluorescent latex microsphere solution containing 1% solids on the amino surface to 9.6 with 0.5M carbonic acid buffer solution of pH 9.6, then add it to step 1), and continue to stir and react for 3 hours. Acquired activated latex microspheres.

[0050] 3) Use a high-speed centrifuge to centrifuge the fluorescent latex microsphere reaction solution at high speed, remove the supernatant of the reaction solution, and ...

Embodiment 2

[0053] The operation method of this example is similar to Example 1, except that the surfactant in step 1) is: 5 mg sodium lauroyl glutamate, 1 mg polyethylene glycol monolaurate, 3 mg sodium stearate .

Embodiment 3

[0055] The operation method of this example is similar to Example 1, except that the surfactant in step 1) is: 6 mg sodium N-acyl glutamate, 4 mg polyethylene glycol monolaurate, 0.5 mg stearin Potassium acid.

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Abstract

The invention discloses an activated fluorescent latex microsphere for a procalcitonin fluorescent immunochromatographic test card, and an application thereof. The preparation method of the activated fluorescent latex microsphere for the procalcitonin fluorescent immunochromatographic test card comprises the steps of 1) adding a surfactant into a buffer solution that is 8-10 in pH value, and then adding dimethylformamide, adding N, N'-dicyclohexylcarbodiimide and N-hydroxysuccinimide; 2) adjusting the dispersion liquid of a fluorescent latex microsphere on the amino surface to be 8-10 in pH value, adding the dispersion liquid into a mixture obtained in the step 1), stirring for reaction, centrifuging to remove a supernatant after the completion of the reaction, and obtaining a surface-activated fluorescent latex microsphere. The surfactant is a mixture of polyethylene glycol fatty acid ester and stearate, wherein the weight ratio of polyethylene glycol fatty acid ester to stearate is 0.5-5:1.

Description

technical field [0001] The invention belongs to the technical field of fluorescence immunochromatography, and in particular relates to an activated fluorescent latex microsphere for a procalcitonin fluorescence immunochromatography detection card and its preparation and application. Background technique [0002] Fluorescence immunochromatography detection card (reagent card) is widely used in the field of rapid detection because of its fast and convenient characteristics. The analyte antibody is labeled with fluorescent latex microspheres, and the latex microspheres have surface electron repulsion and van der Waals forces in the liquid phase to keep the latex particles in the liquid phase evenly distributed. However, after spraying on the glass cellulose film and drying, as the solution is evaporated to dryness, the surface tension pushes the latex particles infinitely closer together, resulting in the disappearance of the intermolecular repulsion, and the increase of the va...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N33/533G01N33/543G01N33/544G01N33/558G01N33/577G01N33/68
CPCG01N33/533G01N33/54313G01N33/54366G01N33/544G01N33/558G01N33/577G01N33/6893G01N2333/585G01N2800/52
Inventor 林斯
Owner BEIJING ELCOTEQ BIO TECH
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