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Method for preventing and controlling clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7

A technology of Bacillus atrophaeus and Brassicaceae, applied in the direction of botany equipment and methods, applications, horticulture, etc., can solve the problems of single, few strains, etc., achieve the reduction of incidence, good decomposing effect, and promote the control effect Effect

Active Publication Date: 2017-02-22
INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] However, in the prior art, there are too few and too single bacterial strains with good control effects on cruciferous crops, mainly focusing on Bacillus subtilis (such as Bacillus subtilis XF-1 and Bacillus subtilis M3) and Bacillus amyloliquefaciens (such as Bacillus amyloliquefaciens Bam22)

Method used

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  • Method for preventing and controlling clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7
  • Method for preventing and controlling clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] (1) Cultivation of strains

[0024] After culturing Bacillus atrophaeus BA-7 on a solid medium, pick a single colony, place it in a liquid medium, and cultivate it with shaking at 30-32°C for 12 hours at a speed of 220rpm, as a seed solution; the liquid medium The ingredients include by mass percentage: 0.3% of yeast extract, 0.2% of beef extract, 0.8% of peptone, 0.3-0.6% of NaCl, the balance is water, pH=7.0-7.2;

[0025] Put the resultant of step 1) into a fermenter for 24 hours of fermentation, the tank temperature is 30° C., and the stirring speed of the fermenter is 250 rpm; the composition of the culture medium in the fermenter includes by mass percentage: soybean powder 4.5%, peptone 0.12% , glucose 0.6%, starch 2.5%, manganese sulfate 0.1%, ferrous sulfate 0.06%, dipotassium hydrogen phosphate 0.2%, calcium carbonate 0.1%, defoamer 0.01%, the balance is water, pH=7.0-7.4.

[0026] (2) Biocontrol effect experiment

[0027] Dilute the Bacillus atrophaeus BA-7 b...

Embodiment 2

[0040] The control method is to spray the Bacillus atrophaeus BA-7 bacterial liquid on the cruciferous crops at an amount of 150-200ml per square meter, and treat the cruciferous crops at least three times. The treatment time is the first day, the seventh day, and the first day after sowing. 14 days; All the other are consistent with embodiment 1. Gained control effect, compared with embodiment 1 gained, maximum deviation is no more than 3%.

experiment example 1

[0048] Farmland: Rapeseed field;

[0049] A row of fungal agent spraying devices are installed on the straw harvester for spraying the fungal agent to the farmland while harvesting the straw.

[0050] Harvest and crush the straw in the farmland, and apply the Bacillus atrophaeus BA-7 bacterial agent to the farmland, and the application rate is 2 trillion bacteria / mu. The bacterial agent is a fermented liquid of Bacillus atrophaeus BA-7, and the concentration of the bacterial liquid is 6-9×10 9 cfu / ml.

[0051] After 45 days of treatment, the straw weight loss rate (full name straw biomass weight loss rate) was detected, and the weight loss rate was 61.3%.

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Abstract

The invention provides a method for preventing and controlling a clubroot disease of cruciferous crops by means of bacillus atrophaeus BA-7. The method comprises any one of the following methods that 1, root irrigation treatment is conducted on the cruciferous crops at least three times with bacillus atrophaeus BA-7 liquid on the first day after sowing, the seventh day after sowing and the fifteenth day after sowing, wherein 80-100 ml of the bacillus atrophaeus BA-7 liquid is applied to each plant every time; 2, spraying treatment is conducted on the cruciferous crops at least three times with bacillus atrophaeus BA-7 liquid on the first day after sowing, the seventh day after sowing and the fourteenth day after sowing, wherein 150-200 ml of the bacillus atrophaeus BA-7 liquid is applied to every square meter of the crops. The cruciferous crops comprise oilseed rapes, Chinese cabbages and broccoli; the classification name of the bacillus atrophaeus is bacillus atrophaeus BA-7, the bacillus atrophaeus BA-7 is preserved in the China Center for Type Culture Collection on December 21, 2015, and the preservation number is CCTCC NO:M 2015765.

Description

technical field [0001] The invention belongs to the technical field of biopesticides, and in particular relates to a method for preventing and treating cruciferous clubroot by using bacillus atrophaeus BA-7. Background technique [0002] In recent years, the harm of clubroot to cruciferous crops has been paid more and more attention by people, and the control methods have been actively researched and explored. Among them, the research and development of biocontrol bacteria is one of the research hotspots. [0003] However, in the prior art, there are too few and too single bacterial strains with good control effects on cruciferous crops, mainly focusing on Bacillus subtilis (such as Bacillus subtilis XF-1 and Bacillus subtilis M3) and Bacillus amyloliquefaciens (such as Bacillus amyloliquefaciens Bam22). [0004] Therefore, it is an urgent technical problem to be solved in this field to seek other biocontrol bacteria that can efficiently prevent and treat clubroot of cruci...

Claims

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Application Information

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IPC IPC(8): A01G13/00
CPCA01G13/00
Inventor 刘勇伍文宪黄小琴张蕾尹全刘红雨周西全
Owner INST OF PLANT PROTECTION SICHUAN ACAD OF AGRI SCI
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