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A kind of Citrobacter y3 with the function of degrading brominated flame retardants and its application

A Citrobacter, functional technology, applied in the direction of bacteria, microbial-based methods, biological water/sewage treatment, etc.

Active Publication Date: 2019-10-01
SUN YAT SEN UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

So far, there is no literature report that Citrobacter sp. has the ability to degrade HBCD

Method used

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  • A kind of Citrobacter y3 with the function of degrading brominated flame retardants and its application
  • A kind of Citrobacter y3 with the function of degrading brominated flame retardants and its application
  • A kind of Citrobacter y3 with the function of degrading brominated flame retardants and its application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Example 1 Isolation and Identification of Citrobacter Y3

[0037] 1. Enrichment and isolation of strains

[0038] Continuous-flow anaerobic reaction with hexabromocyclododecane as a contaminant grown for 2 years from the laboratory

[0039] Take out 5ml of the mud-water mixture from the instrument and culture it in a standard serum bottle, and at the same time add an inorganic salt culture solution containing 5mg / L hexabromocyclododecane (see Table 1 for the composition of the culture solution), anaerobic at 30°C, and a shaker with a rotation speed of 120rap After one week of enrichment and acclimatization in medium, transfer the inorganic salt medium containing 8mg / L hexabromocyclododecane with 10% inoculation amount and then acclimatize for a week, and gradually increase the concentration of hexabromocyclododecane for acclimatization by analogy, hexabromocyclododecane The use concentrations of cyclododecane were 10, 15, and 20 mg / L, respectively. After acclimatizati...

Embodiment 2

[0091] Example 2 Degradation test of Citrobacter Y3 to hexabromocyclododecane under anaerobic co-metabolism conditions

[0092] 1. For the isolated and purified Citrobacter Y3 under anaerobic conditions and the serum bottle experiment for the degradation of HBCD without adding co-metabolism substrates, samples were taken every 2 days until the eighth day to detect hexabromocyclododecane The degradation rate of cyclododecane and the growth of strain OD 600nm .

[0093] 2. After experiments, the inventors found that under the condition of using hexabromocyclododecane as a single carbon source, the growth of the bacteria was very slow within eight days, and the degradation efficiency of hexabromocyclododecane was only about 40%. This shows that HBCD is a refractory persistent organic compound, and the strain may lack the corresponding enzyme in the body, so it is difficult to use HBCD as an energy source to support its growth. Therefore, the method of adding co-metabolism subst...

Embodiment 3

[0096] Example 3 Effects of Different Concentrations of Co-Metabolite Substrates on the Degradation of Hexabromocyclododecane by Citrobacter Y3

[0097]The inventors also explored the effects of different concentrations and different types of co-metabolite substrates on the degradation of hexabromocyclododecane by strain Y3, respectively setting up gradient experiments with different concentrations of glucose, maltose, sucrose, starch and sodium citrate, and comparing their effects on the hexabromocyclododecane. The impact of bromocyclododecane degradation efficiency, determine the optimum range or optimum ratio under optimum degradation conditions.

[0098] Firstly, five different concentrations (10, 100, 500, 1000, 2000mg / L) were set according to the different ratios of the co-metabolism substrate and the pollutant HBCD to explore the optimum between the co-metabolism substrate and the pollutant substrate. relation. As we all know, the ratio of growth substrate and non-grow...

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Abstract

The present invention relates to a Citrobacter Y3 having the function of degrading hexabromocyclododecane and its application. The Citrobacter Y3 was preserved in the China Center for Type Culture Collection on April 8, 2016, and the preservation number is CCTCC NO : M 2016182; the Citrobacter Y3 is a Gram-negative bacterium, and the colonies on the MSM plate are round, smooth, moist and easy to stir up, slightly conglutinated, and the color is light yellow to milky white, opaque. The Citrobacter Y3 provided by the invention has strong degradation ability to brominated flame retardants under the condition of anaerobic co-metabolism, and the bacterial strain is suitable for controlling the pollution of brominated flame retardants in the environment, and can achieve the purpose of environmental restoration.

Description

technical field [0001] The invention relates to the field of Citrobacter, in particular to a Citrobacter capable of degrading brominated flame retardants and an application thereof. Background technique [0002] Hexabromocyclododecane is one of the three largest brominated flame retardants in the world. It is an additive brominated flame retardant, which makes it easy to release the substance to the environment during the use of the product. So far, HBCD has accumulated to a certain concentration in the human living environment, and has been widely detected in natural environments such as air, water, sediment, soil, and organisms. In addition, as a refractory persistent organic compound, hexabromocyclododecane (HBCD) has three characteristics: bioaccumulation, long-distance migration, and time persistence, which have been confirmed by scholars' research. In addition, HBCD may also cause liver toxicity, neurotoxicity and endocrine toxicity to organisms. There is no doubt th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C02F3/34C02F3/28C12R1/01C02F101/36
CPCC02F3/28C02F3/34C02F2101/36C12N1/205C12R2001/01
Inventor 彭星星余蓝魏东洋贾晓珊
Owner SUN YAT SEN UNIV
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