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Living single cell Raman spectrum detection chip

A technology of Raman spectroscopy detection and Raman spectroscopy, which is applied in the field of cell detection chips, can solve problems such as difficulty in use, high cost of use, and cumbersome processing of microfluidic chips, and achieve the advantages of convenient operation, simple tools, and guaranteed stability Effect

Inactive Publication Date: 2017-02-22
CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the microfluidic chip is not only cumbersome to process, but also requires complex software and hardware systems such as microfluidic pumps, sample injection, feedback and recovery control during use, which is costly and difficult to use in general laboratories.

Method used

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  • Living single cell Raman spectrum detection chip
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  • Living single cell Raman spectrum detection chip

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A live single-cell Raman spectrum detection chip 21, its structural features are as follows: the chip 21 is composed of a quartz substrate without Raman spectrum characteristics, one side of the substrate has a micro-pits structure arranged in a quadrilateral array, and the micro-pits The surface diameter is 15.4um and the depth is 4.4um, which is used to separate and fix neuroblastoma single cells, and the diameter of neuroblastoma single cells is 11um;

[0018] The shape of the micro-pit in the chip 21 is a spherical crown, and it is prepared by a micro-lithography processing method. The specific process steps include: first spin-coating a photoresist on the surface of the cleaned quartz substrate, and then using the same micro-pit distribution. The photolithographic mask plate is exposed, and after development, it is placed in hydrofluoric acid etching solution to etch the required micro-pit array structure, such as figure 1 shown.

[0019] Using the invented chip 2...

Embodiment 2

[0021] A live single-cell Raman spectrum detection chip has the following structural features: the chip is composed of a magnesium fluoride substrate without Raman spectrum characteristics, and one side of the substrate has a micro-pit structure arranged in a hexagonal array. The surface diameter of the pit is 13.2um, and the depth is 7.2um, which is used to separate and fix ovarian cancer cells, and the diameter of ovarian cancer cells is 12um;

[0022] The shape of the micro-pits in the chip is cylindrical, and it is prepared by micro-lithography processing method. The specific process steps include: first, spin-coat photoresist on the surface of the cleaned quartz substrate, and then use the same light distribution as the micro-pits to The mask plate is etched for exposure, and after development, a reactive ion etching process is used to etch out the required micro-pit array structure.

[0023] Using the invented chip, the Raman spectrum detection method of living single ce...

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Abstract

The invention provides a living single cell Raman spectrum detection chip. The living single cell Raman spectrum detection chip is formed by a substrate with micro-pit arrays and without Raman scattering characteristic peaks. Living single cells are dripped on the surface of the chip, after moving and falling into micro-pits, the cells are allowed to remain motionless, then Raman spectrum measurement is conducted by a microscope, and thus Raman spectrum of the living single cells is obtained. The chip is used for living single cell Raman spectrum measurement, and during the measurement, a complex control system required by microfluidic control is not needed. The living single cells can be added by dripping, the operation is simple, and the chip can be widely used in the field of biomedical detection.

Description

technical field [0001] The invention relates to a cell detection chip, which belongs to the field of microstructure-based chips for Raman spectrum detection of living single cells. Background technique [0002] Cells are the basic unit of life. The detection of living cells, especially single-cell detection, is very important for the precise diagnosis of human diseases, the development of precise drugs, and the study of physiological microscopic processes. However, a single cell is a complex system that contains many biochemical substances. At the same time, the size of a single cell is small, usually on the order of several microns to tens of microns. Therefore, how to accurately detect single cells while ensuring normal physiological activity of cells The composition of substances inside cells and the analysis of their physiological functions are a big problem. [0003] Raman spectroscopy is an inelastic scattering spectrum of a substance, usually excited by a laser, and ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/65
CPCG01N21/65
Inventor 夏良平汤明杰张为国魏东山崔洪亮杜春雷
Owner CHONGQING INST OF GREEN & INTELLIGENT TECH CHINESE ACADEMY OF SCI
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