Microbial fungicide and preparation method and application thereof

A bactericide and microbial technology, applied in the field of pesticides, to achieve the effects of improving extraction efficiency, high-efficiency preparation methods, and low biological hazards

Inactive Publication Date: 2017-03-08
WUHAN INSTITUTE OF TECHNOLOGY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

There are also reports on the antibacterial activity of the mushroom fermentation liquid, b

Method used

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  • Microbial fungicide and preparation method and application thereof
  • Microbial fungicide and preparation method and application thereof

Examples

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Embodiment 1

[0033] The preparation of mushroom fermented liquid extract bactericide, concrete steps are as follows:

[0034] 1) Prepare PDA medium (Potato Dextrose Agar Medium (PDA): 200g of potatoes, 20g of glucose, 18g of agar, 1000ml of distilled water, natural pH), put into test tubes, at 121°C, 0.1MPa, Sterilize for 20 minutes at high temperature and damp heat, and set aside the inclined surface for later use. Inoculate shiitake mushroom strains (purchased from the Strain Experimental Center of Huazhong Agricultural University, Wuhan, Hubei Province) on a PDA slant, activate and culture at 28°C for 7 days, and then transfer the activated strains to the poured PDA medium culture dish , activated and cultured at 28°C for 7 days, and used as activated mushroom strains for future use.

[0035] 2) Prepare liquid complete medium (medium formula: glucose 20g, peptone 2g, yeast extract 2g, KH 2 PO 4 0.46g, K 2 HPO 4 1g, MgSO 4 ·7H 2 O 0.5g. Distilled water 1000mL, pH natural), 250mL ...

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Abstract

The invention relates to a microbial fungicide and a preparation method and an application thereof. The method comprises the following steps: extracting a mushroom broth by an extractant, concentrating the material to a paste, and the paste and an auxiliary agent are used according to the mass volume ratio of 1g: 5mL to prepare the product. The mushroom broth extract fungicide contains natural antibacterial active metabolite, has wide-spectrum antibacterial and antifungal activities, can be used for inhibiting escherichia coli, pseudomonas solanacearum, tritirachium album, tobacco alternaria alternate and tobacco phytophthora, and can be used for preparing a food antiseptic or a biological pesticide. The preparation method is efficient, the obtained fungicide is the microbial source fungicide, and has the advantages of low toxicity and low residue.

Description

technical field [0001] The invention specifically relates to a microbial bactericide and its preparation method and application, belonging to the technical field of pesticides. Background technique [0002] Higher fungi contain a variety of antibacterially active metabolites and are considered to be naturally nontoxic. In recent years, with the increasing demand for organic food and the emphasis on environmental issues, people have become more and more aware of the importance of biopesticides and biofungicides, and have also become enthusiastic about the development and development of natural and non-toxic biofungicides. application. [0003] There are many studies on the extraction of antibacterial active substances from the fruiting bodies of edible fungi, but less research on the isolation of antibacterial active substances from the fermentation broth of edible fungi. Studies have reported that the fermented liquids of Pteris cerevisiae, Ganoderma lucidum, Flammulina ve...

Claims

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Application Information

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IPC IPC(8): A01N63/04A01N25/04A01P1/00A01P3/00A23L3/3571
CPCA01N25/04A01N63/30A23L3/3571A23V2002/00A23V2200/10A23V2250/208A01N2300/00
Inventor 胡国元李伟伟龚吉红
Owner WUHAN INSTITUTE OF TECHNOLOGY
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