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Externally used gel preparation containing human umbilical cord mesenchymal stem cell extract as well as preparation method and application of externally used gel preparation

A technology of mesenchymal stem cells and gel preparations, which is applied in the field of external gel preparations containing human umbilical cord mesenchymal stem cell extracts and its preparation, can solve the problems of harsh survival conditions for active cells, easy-to-death living cells, discounts, etc. Achieve the effects of shortening healing time, eliminating inflammation, and promoting ulcer healing

Active Publication Date: 2017-03-08
天津捷渲生物医药科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to the harsh survival conditions of active cells, it is easy to die, so the role played by living cells will be greatly reduced.

Method used

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  • Externally used gel preparation containing human umbilical cord mesenchymal stem cell extract as well as preparation method and application of externally used gel preparation
  • Externally used gel preparation containing human umbilical cord mesenchymal stem cell extract as well as preparation method and application of externally used gel preparation
  • Externally used gel preparation containing human umbilical cord mesenchymal stem cell extract as well as preparation method and application of externally used gel preparation

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preparation example Construction

[0037] The preparation method of the above-mentioned external gel preparation containing human umbilical cord mesenchymal stem cell extract comprises the following steps:

[0038] (1) Preparation of human umbilical cord mesenchymal stem cell extract: select cells from the cell bank for recovery, use phenol red-free DMEM / DF12 and 10% fetal calf serum as a complete medium, and T75 cell culture bottle at 37 °C, 5% CO 2 Carry out culture, when the growth degree of the cells reaches 70%-80%, wash the cells twice with normal saline, perform starvation culture with compound electrolyte injection for 12-24 hours, and collect the cells for culture after the growth degree of the cells reaches 90%-95% Centrifuge the supernatant at 3000-4500g for 5-8min, filter with a 0.22um filter membrane, add human serum albumin according to the mass volume ratio of 2%-4%, mix well and set aside;

[0039] (2) Gel solution preparation: according to the ratio, the medicinal polymer stabilizer, moisturiz...

Embodiment 1

[0044] (1) Human umbilical cord mesenchymal stem cell extract: select cells from the cell bank for resuscitation, use phenol red-free DMEM / DF12 and 10% fetal bovine serum as a complete medium, and place a T75 cell culture bottle at 37°C , 5% CO 2 Culture until the cell growth reaches 70%-80% (see figure 1 ), washed the cells twice with normal saline, starved and cultured with compound electrolyte injection for 12 hours, and after the cell growth reached 90%--95%, collected the cell culture supernatant and centrifuged at 3000g for 8min, filtered through a 0.22um filter membrane Filter, add human serum albumin according to the mass volume ratio of 2%, mix well and set aside;

[0045] (2) Preparation of gel solution: according to the proportion, dissolve 2.5g of medicinal polymer stabilizer sodium alginate, 5ml of sterile moisturizing agent propylene glycol, and 5ml of transdermal absorption promoter dimethyl sulfoxide into 90ml of compound electrolyte solution , prepared into ...

Embodiment 2

[0048] (1) Human umbilical cord mesenchymal stem cell extract: select cells from the cell bank for resuscitation, use phenol red-free DMEM / DF12 and 10% fetal bovine serum as a complete medium, and place a T75 cell culture bottle at 37°C , 5% CO 2 Carry out culture, when the growth degree of the cells reaches 70%-80%, wash the cells twice with normal saline, perform starvation culture with compound electrolyte injection for 12 hours, when the growth degree of the cells reaches 90%-95%, collect the cells on the culture medium Centrifuge at 4000g for 6 minutes, filter with a 0.22um filter membrane, add human serum albumin according to the mass volume ratio of 3%, mix well and set aside;

[0049](2) Preparation of gel solution: According to the ratio, 5g of medicinal polymer stabilizer sodium hyaluronate, 7.5ml of sterile moisturizing agent propylene glycol, and 7.5ml of transdermal absorption accelerator dimethyl sulfoxide were dissolved in 85ml of compound In the electrolyte so...

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Abstract

The invention discloses an externally used gel preparation containing human umbilical cord mesenchymal stem cell extract as well as a preparation method and an application of the externally used gel preparation. The externally used gel preparation contains the human umbilical cord mesenchymal stem cell extract and a gel solution, wherein the human umbilical cord mesenchymal stem cell extract is the extract obtained after starvation culture; the gel solution contains sodium alginate, a compound electrolyte injection, a humectant and a penetration enhancer, and a stable and homogeneous gelatinous substance is formed. The externally used gel preparation can be used for preparing drugs for treating skin ulcer, burns, scalds, eczema, trauma and other diseases.

Description

technical field [0001] The invention relates to a stem cell extract preparation, especially an external gel preparation containing human umbilical cord mesenchymal stem cell extract and its preparation method and application. Background technique [0002] Mesenchymal stem cells (MSCs) are a group of heterogeneous cell populations derived from stroma, which can be obtained from most tissues of the human body. They have the ability of self-renewal, tissue repair, and immune regulation, and can differentiate to the mesoderm lineage, such as adipocytes, bone cells, chondrocytes, etc., and can also differentiate to other germ layer lineage cells, such as epidermal cells \ vascular endothelium Cell Differentiation. [0003] The mesenchymal stem cell culture system contains a variety of active ingredients, such as: stem cell growth factor (SCF), fibroblast growth factor (FGF), endothelial cell growth factor (VEGF), epidermal growth factor (EGF), cell Colony-stimulating factor, in...

Claims

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Application Information

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IPC IPC(8): A61K35/28A61K9/06A61K47/36A61P17/02A61P17/00C12N5/0775
CPCA61K9/0014A61K9/06A61K35/28A61K47/36C12N5/0665C12N2501/998
Inventor 韩洪起鲁振宇张冰晶秦臻熊亮刘俊江徐悦黄文敬
Owner 天津捷渲生物医药科技有限公司
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