Looking for breakthrough ideas for innovation challenges? Try Patsnap Eureka!

Collagen micro fiber and preparation method thereof

A technology of collagen and collagen protein, applied in the field of materials, can solve problems such as long hemostasis time, wound infection and suppuration, wound adhesion, etc., achieve the effect of qualified heavy metal content, simplified preparation process, and broad application prospects

Active Publication Date: 2017-03-08
YANTAI ZHENGHAI BIO TECH
View PDF5 Cites 3 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The tourniquet is a life-saving device that can control bleeding, but it cannot be used or is invalid for many parts of the human body, such as the chest, abdomen, head, neck, etc.
Commonly used clinical hemostatic materials such as hemostatic gauze and hemostatic bandage have limitations: such as longer hemostasis time, easy to adhere to the wound and difficult to change dressings, powerless to wound infection and suppuration, and irregular shape, deep, narrow, arterial The hemostatic effect of common wounds such as rupture is not ideal

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Collagen micro fiber and preparation method thereof
  • Collagen micro fiber and preparation method thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] 1. Removal of non-collagen components in raw materials

[0049] 1. The pigskins that have just been slaughtered are collected by special personnel from the slaughterhouses under standardized management, and the contact with pollutants should be avoided as far as possible, and the pigskins should be frozen and stored immediately after collection;

[0050] 2. After the frozen material is thawed, it is fully cleaned, and the part that is not suitable for processing is removed at the same time;

[0051] 3. Use 2% acetic acid by mass to soak the thawed raw materials for 10 hours. The temperature of the solution is controlled at about 10°C. The purpose is to destroy the cell structure, remove cells and non-collagen protein structures; use the processed raw materials The PBS buffer solution with a pH value of 7.4 was soaked and washed for 60 minutes, so that the final pH value was 7.4;

[0052] 4. After cleaning the raw materials, use 0.6g / 100ml of surfactant TrironX-100 aque...

Embodiment 2

[0062] 1. Removal of non-collagen components in raw materials

[0063] 1. The pigskins that have just been slaughtered are collected by special personnel from the slaughterhouses under standardized management, and the contact with pollutants should be avoided as far as possible, and the pigskins should be frozen and stored immediately after collection;

[0064] 2. After the frozen material is thawed, it is fully cleaned, and the part that is not suitable for processing is removed at the same time;

[0065] 3. Use 3% acetic acid by mass to soak the thawed raw materials for 5 hours. The temperature of the solution is controlled at about 10°C. The purpose is to destroy the cell structure, remove cells and non-collagen protein structures; use the processed raw materials The PBS buffer solution with a pH value of 7.4 was soaked and washed for 60 minutes, so that the final pH value was 7.4;

[0066] 4. After washing the raw materials, use 1g / 100ml TrironX-100 solution to soak for 4...

Embodiment 3

[0075] Embodiment 3: Physical and chemical property detection of collagen microfibrils

[0076] 1. Water absorption rate and water absorption speed test

[0077] Get the sample after freeze-drying prepared by embodiment 1, embodiment 2 respectively, about 100mg, weighing m 1 , accurate to 0.001g. Then add it to a flat plate with excess physiological saline, and record the time when the sample is completely absorbed with water, that is, the water absorption speed. Then use filter paper to absorb excess water on the surface of the sample, and weigh m 2 . Calculate the ratio of the absorbed water weight to the sample weight, repeat the measurement 5 times, and obtain the average value to obtain the water absorption rate of the collagen microfibrils relative to its own weight (Formula 1). The specific results are shown in Table 1.

[0078] Water absorption = (m 2 -m 1 ) / m 1 *100% (1)

[0079] It can be found that the water absorption speed and water absorption rate of the ...

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
water absorptionaaaaaaaaaa
Login to View More

Abstract

The invention discloses a collagen micro fiber and a preparation method thereof. Provided are the collagen micro fiber and the preparation method thereof. The method comprises the following steps: 1) taking an isolated mammalian dermis and / or subcutaneous tissue layer as a raw material, and removing non-collagen components in the raw material to obtain a crude product; and 2) removing non collagen in the crude product obtained in the step 1), to obtain the collagen micro fiber. The preparation process of the method is simplified, and introduction of complex production equipment is not needed, so that difficult control of microorganisms, heavy metals and other factors in the production process is avoided, and the requirement of industrialized production can be met; the collagen micro fiber is suitable for large-area diffuse hemorrhage, has he advantages of fast hemostatic speed, high hemostatic efficiency, qualified heavy metal content and the like, and has wide application prospects.

Description

technical field [0001] The invention belongs to the field of materials, and relates to a collagen microfiber and a preparation method thereof. Background technique [0002] In the first aid treatment of sudden accidents in daily life, the trauma hemostasis during the operation of patients in the hospital, especially the rescue of wounded soldiers in war, the local rapid and effective hemostasis of patients is very important. Because uncontrolled bleeding is the leading cause of accidents, intraoperative hemorrhage, or battlefield casualties. In the Iraq war, 50% of the casualties on the battlefield were caused by excessive bleeding. Early control of bleeding becomes the best strategy to reduce patient casualties. [0003] Trauma hemostatic materials are used in the treatment of traumatic bleeding and are commonly used items in rescue. The research on wound hemostatic materials is of great significance for trauma treatment. The tourniquet is a life-saving device that can c...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
Patent Type & Authority Applications(China)
IPC IPC(8): A61L26/00
Inventor 闫玉芳董佳桓孙先昌郭松王彬姜洪磊
Owner YANTAI ZHENGHAI BIO TECH
Who we serve
  • R&D Engineer
  • R&D Manager
  • IP Professional
Why Patsnap Eureka
  • Industry Leading Data Capabilities
  • Powerful AI technology
  • Patent DNA Extraction
Social media
Patsnap Eureka Blog
Learn More
PatSnap group products

Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.

© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com