Method for reducing macrolide resistance gene and intI1 abundance in chicken manure compost

Abstract

The invention provides a method for reducing macrolide resistance gene (ermB, ermF, ermX, ermQ) and intI1 abundance in chicken manure compost. The method comprises the steps of adding wheat straws in chicken manure as amendment, adding two different types of surface active agent (rhamnolipid and tween 80), mixing fresh chicken manure and crushed wheat straws in uniformity, regulating the initial C / N ratio to be roughly 30:1, adjusting the initial rate of water content of an aqueous solution using 0.15% rhamnolipid (RL) to 50-60%, adjusting the rate of water content of a solution using 0.15% tween 80 (Tw) to 55-60%, adjusting the rate of water content of a mixture solution using both 0.15% rhamnolipid and tween 80 (R+T) to 55-60%, stirring the piled materials fully and evenly, conducting the composting, using manual turning to ensure the exposure to oxygen, composting for 30 days, at the 26 day the compost being thoroughly decomposed, after the completion of the composting, the macrolide resistance gene being reduced by more than 85%, the intI1 abundance being reduced by more than 90%. The method reduces macrolide resistance gene and intI1 abundance in chicken manure compost, and has the advantages of being simple in equipment, and convenient in operation. The compost fertilizer prepared by the method has the advantages of being stable in quality, and being high in fertilizing effect.

Description

technical field [0001] The invention belongs to the technical field of farm solid waste treatment, in particular to a method for reducing the abundance of macrolide resistance genes (ermB, ermF, ermX, ermQ) and intI1 in chicken manure compost. Background technique [0002] With the development of large-scale and intensive livestock and poultry farming, antibiotics have been widely used in livestock and poultry farming as growth promoters and disease prevention drugs. However, these antibiotics are not easy to be absorbed by livestock and poultry, and only a small amount can participate in the metabolism of animals and be effectively used. Most of them will be excreted from the animal body in the form of drug prototype or metabolites with livestock and poultry feces and urine, resulting in antibiotic residues in livestock and poultry feces volume gradually increased. These untreated livestock and poultry manures with a large amount of antibiotic residues are directly applied...

AI Technical Summary

Problems solved by technology

Studies have shown that surfactants can increase the number of microorganisms and enzyme activities when applied to compost, but the effect on ARGs has not been reported yet.

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