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Novel composite protective agent for freeze-dried preparation of staphylococcus aureus

A staphylococcus and aureus technology, applied in the field of microorganisms, can solve the problem of unsatisfactory protective effect of protective agents, achieve the effect of inhibiting oxidation and free radical formation, stable structure, stable structure and function

Active Publication Date: 2017-03-15
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The protective effects of these protective agents are not ideal, but in fact, the effect of the freeze-dried protective agent will show certain differences with the composition and ratio of the protective agent. Therefore, the development of a unique and highly effective Staphylococcus aureus frozen Dry protectant is of great significance

Method used

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  • Novel composite protective agent for freeze-dried preparation of staphylococcus aureus
  • Novel composite protective agent for freeze-dried preparation of staphylococcus aureus
  • Novel composite protective agent for freeze-dried preparation of staphylococcus aureus

Examples

Experimental program
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Effect test

Embodiment 1

[0020] The composition of the novel compound protective agent for freeze-drying preparation of Staphylococcus aureus of this embodiment is: trehalose 2g, sodium glutamate 12g, L-cysteine ​​hydrochloride 0.5g, bovine serum albumin 0.25g, distilled water 100ml.

[0021] Its preparation method:

[0022] 1) Add 2g of trehalose and 12g of sodium glutamate in distilled water according to the amount, sterilize at 113°C for 15min, and cool to room temperature;

[0023] 2) Dissolve 0.5g of L-cysteine ​​hydrochloride and 0.25g of bovine serum albumin in distilled water according to the amount, and then filter and sterilize with a 0.22μm filter membrane;

[0024] 3) Mix the solutions prepared in steps 1) and 2), and adjust the total mass volume percentage of water to 100%, to obtain the Staphylococcus aureus composite lyoprotectant.

[0025] After activated culture, centrifugation and washing of Staphylococcus aureus, the sludge was collected, mixed with a protective agent before freez...

Embodiment 2

[0028] The composition of the novel compound protective agent for freeze-drying preparation of Staphylococcus aureus in this example is: trehalose 4g, sodium glutamate 12g, L-cysteine ​​hydrochloride 1g, bovine serum albumin 0.75g, distilled water 100ml .

[0029] Its preparation method:

[0030] 1) Add 4g of trehalose and 12g of sodium glutamate in distilled water according to the amount, sterilize at 113°C for 15min, and cool to room temperature;

[0031] 2) Dissolve 1g of L-cysteine ​​hydrochloride and 0.75g of bovine serum albumin in distilled water according to the amount, and then filter and sterilize with a 0.22μm filter membrane;

[0032] 3) The solutions prepared in steps 1) and 2) were mixed, and the total mass volume percentage of water was adjusted to 100% to obtain a Staphylococcus aureus lyoprotectant.

[0033] Staphylococcus aureus was activated, cultured, centrifuged, and cleaned, and the sludge was collected, mixed with a protective agent before freeze-dryin...

Embodiment 3

[0036] The composition of the novel composite protective agent for freeze-drying preparation of Staphylococcus aureus in this example is: trehalose 6g, sodium glutamate 18g, L-cysteine ​​hydrochloride 1g, bovine serum albumin 0.25g, distilled water 100ml .

[0037] Its preparation method:

[0038] 1) Add 6g of trehalose and 18g of sodium glutamate in distilled water according to the amount, sterilize at 113°C for 15min, and cool to room temperature;

[0039] 2) Dissolve 1g of L-cysteine ​​hydrochloride and 0.25g of bovine serum albumin in distilled water according to the amount, and then filter and sterilize with a 0.22μm filter membrane;

[0040] 3) The solutions prepared in steps 1) and 2) were mixed, and the total mass volume percentage of water was adjusted to 100% to obtain a Staphylococcus aureus lyoprotectant.

[0041] Staphylococcus aureus was activated, cultured, centrifuged, and cleaned, and the sludge was collected, mixed with a protective agent before freeze-dryi...

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Abstract

The invention provides a novel composite protective agent for freeze-dried preparation of staphylococcus aureus. The protective agent mainly contains the ingredients by content: 2% to 6% of trehalose, 12% to 18% of monosodium glutamate, 0.5% to 1.5% of L-cysteine hydrochloride and 0.25% to 0.75% of bovine serum albumin. The freeze-drying protective agent is mainly applied to the preparation of freeze-dried bacterium powder of the staphylococcus aureus. The bacterium powder of the staphylococcus aureus is obtained through subjecting staphylococcus aureus thalli to culture, centrifugation and washing, then, carrying out collecting, carrying out mixing with the protective agent before carrying out freeze-drying, carrying out subpackaging, carrying out pre-freezing for 2 to 3 hours at the temperature of -80 DEG C, and then, carrying out freeze drying for 16 to 18 hours. Through using the protective agent during the preparation of the bacterium powder, the freezing resistance of the thalli can be improved, so that the survival rate of the bacterium powder in a freeze-drying treatment process is increased greatly. Through treating the freeze-drying process for the staphylococcus aureus with the freeze-drying protective agent provided by the invention, the survival rate of the obtained freeze-dried bacterium powder of the staphylococcus aureus can reach 99.82%.

Description

technical field [0001] The invention relates to a novel composite protective agent for freeze-drying preparation of Staphylococcus aureus in the technical field of microorganisms. Background technique [0002] Staphylococcus aureus is one of the important pathogenic bacteria that cause bacterial food poisoning. It is widely distributed in nature. At present, the inspection of Staphylococcus aureus has been taken as a routine inspection item in the hygienic standards of food and cosmetics. [0003] Staphylococcus aureus is mostly supplied to the market in the form of freeze-dried powder. The freeze-dried protective agents for Staphylococcus aureus widely used at home and abroad include soluble starch, skimmed milk powder, trehalose, lactose, maltodextrin, sodium glutamate, and sodium ascorbate , vitamin C, etc. According to literature reports, with skim milk powder as a single protective agent, the survival rate of Staphylococcus aureus after freeze-drying is less than 50%,...

Claims

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Application Information

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IPC IPC(8): C12N1/04C12N1/20C12R1/445
CPCC12N1/04C12N1/20
Inventor 李金霞杨海丽都海渤刘波刘毅胡海蓉李赞程池
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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