Novel composite protective agent for freeze-dried preparation of staphylococcus aureus
A staphylococcus and aureus technology, applied in the field of microorganisms, can solve the problem of unsatisfactory protective effect of protective agents, achieve the effect of inhibiting oxidation and free radical formation, stable structure, stable structure and function
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
Example Embodiment
[0019] Example 1
[0020] The composition of the novel composite protective agent for freeze-drying preparation of Staphylococcus aureus in this embodiment is: 2 g of trehalose, 12 g of sodium glutamate, 0.5 g of L-cysteine hydrochloride, 0.25 g of bovine serum albumin, and distilled water 100ml.
[0021] Its preparation method:
[0022] 1) Add 2g of trehalose and 12g of sodium glutamate to distilled water according to the amount, sterilize at 113°C for 15min, and cool to room temperature;
[0023] 2) Dissolve 0.5g of L-cysteine hydrochloride and 0.25g of bovine serum albumin in distilled water, and then filter and sterilize with a 0.22μm filter membrane;
[0024] 3) Mix the solutions prepared in steps 1) and 2), and adjust the total mass volume percentage of water to be 100% to obtain a Staphylococcus aureus composite freeze-drying protective agent.
[0025] After activating, culturing, centrifuging and washing Staphylococcus aureus, collecting the bacterial slurry, mi...
Example Embodiment
[0027] Example 2
[0028] The composition of the novel composite protective agent for freeze-drying preparation of Staphylococcus aureus in this embodiment is: trehalose 4g, sodium glutamate 12g, L-cysteine hydrochloride 1g, bovine serum albumin 0.75g, distilled water 100ml .
[0029] Its preparation method:
[0030] 1) Add 4g of trehalose and 12g of sodium glutamate to distilled water according to the amount, sterilize at 113°C for 15min, and cool to room temperature;
[0031] 2) Dissolve 1 g of L-cysteine hydrochloride and 0.75 g of bovine serum albumin in distilled water according to the amount, and then filter and sterilize with a 0.22 μm filter membrane;
[0032] 3) Mix the solutions prepared in steps 1) and 2), and adjust the total mass volume percentage of water to be 100% to obtain Staphylococcus aureus freeze-drying protection agent.
[0033] After activating, culturing, centrifuging and washing Staphylococcus aureus, collecting the bacterial slurry, mixing wit...
Example Embodiment
[0035] Example 3
[0036] The composition of the novel composite protective agent for freeze-drying preparation of Staphylococcus aureus in this embodiment is: trehalose 6g, sodium glutamate 18g, L-cysteine hydrochloride 1g, bovine serum albumin 0.25g, distilled water 100ml .
[0037] Its preparation method:
[0038] 1) Add 6g of trehalose and 18g of sodium glutamate to distilled water according to the amount, sterilize at 113°C for 15min, and cool to room temperature;
[0039] 2) Dissolve 1 g of L-cysteine hydrochloride and 0.25 g of bovine serum albumin in distilled water according to the amount, and then filter and sterilize with a 0.22 μm filter;
[0040] 3) Mix the solutions prepared in steps 1) and 2), and adjust the total mass volume percentage of water to be 100% to obtain Staphylococcus aureus freeze-drying protection agent.
[0041] After activating, culturing, centrifuging and washing Staphylococcus aureus, collecting the bacterial slurry, mixing with a prote...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap