ANS salt composite releaser and kit for detecting cortisol in blood

A cortisol and reagent kit technology, applied in the field of immunoassay, can solve the problems of high cleaning requirements, weakened luminescent signal, interference, etc., and achieve the effects of high anti-interference, less time for luminescence, and accurate content

Active Publication Date: 2017-03-15
DIRUI MEDICAL TECH CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0010] Disadvantages: The luminescence of isoluminol and its derivatives is flash type, and its luminescence intensity mainly depends on the concentration of enzyme in the immune reaction. If no catalyst is used or the amount of catalyst is insufficient, the luminescent signal will become weak, and the amount of catalyst is difficult to control ;Secondly, isoluminol is interfered by hemolysis in the sample, which affects the determination of free cortisol content in blood
[0013] Insufficient: too sensitive to environmental factors and other non-specific reactions; too sensitive to metal chelates of metal ions, EDTA or other anticoagulants, preservatives; deposition of reaction by-products is also a potential source of interference; A sample of -7 is very sensitive ;O 2 Under the condition of adding voltage, it emits light under the condition of 620nm, and the electrochemiluminescence is interfered by the dissolved oxygen in the reaction system; the

Method used

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  • ANS salt composite releaser and kit for detecting cortisol in blood
  • ANS salt composite releaser and kit for detecting cortisol in blood
  • ANS salt composite releaser and kit for detecting cortisol in blood

Examples

Experimental program
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Embodiment

[0052] 1. Preparation of magnetic bead solid-phase reagent coated with cortisol antibody

[0053] 1) Resuspend 5 mg of magnetic beads with a particle size of 2.0 μm in 50 mM MES buffer with a pH of 6.0±0.1, use a vortex shaker to mix, shake the centrifuge tube fully (note: there should be no magnetic beads on the cap of the centrifuge tube) residue) placed on the magnetic separation rack until the supernatant was completely thorough, then carefully discard the supernatant with a micropipette, and repeat the operation twice.

[0054] 2) Resuspend the magnetic beads to the target volume with 50mM MES buffer solution with a pH of 6.0±0.1, mix them with a vortex shaker, add an appropriate amount of cortisol antibody according to the coating ratio of 1.5% after shaking fully, and use a vortex shaker to mix quickly and fully Then add 50mM MES buffer solution with a pH of 6.0±0.1 and 5M ammonium sulfate buffer solution, use a vortex shaker to quickly and fully mix, and use a mixer to...

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Abstract

The invention discloses an ANS salt composite releaser and a kit for detecting cortisol in blood. The kit comprises cortisol-antibody-coated magnetic beads, an acridinium-ester-labeled cortisol BSA (bovine serum albumin) composite, an ANS composite releaser and a cortisol calibration product. The ANS salt composite releaser converts the combined cortisol in the human blood sample into free cortisol, and the free cortisol content is detected by acridinium ester chemiluminescence. The ANS salt composite releaser can be combined with different glucocorticosteroid proteins, and can sufficiently release the combined cortisol in the blood. The acridinium ester chemiluminescence is flash luminescence, requires short time for luminescence, and can completely capture the photons generated by reaction. The acridinium ester is a synthetic substance, and has higher interference resistance to sample interfering materials. The acridinium ester label for detecting the free cortisol content is very sensitive, so that the total cortisol content detection in the blood sample is more accurate.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to an ANS salt compound releasing agent and a kit for detecting cortisol in blood. Background technique [0002] Cortisol, also known as "hydrocortisone", is a glucocorticoid produced by the adrenal gland in response to stress. When a person ingests cholesterol from food, under the action of the body's stress response, cholesterol will be converted into cortisol through the "glucocorticoid system" in the adrenal gland, and secreted into the blood in a free form. Cortisol has the function of regulating carbohydrate metabolism; immunosuppressive and anti-inflammatory effects; negative feedback regulation of corticotropin-releasing hormone secreted by hypothalamus and corticotropin secreted by pituitary. Therefore, by detecting the content of cortisol, the functional state of the adrenal gland can be diagnosed, and the functional state of the pituitary gland can be indirectly ...

Claims

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Application Information

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IPC IPC(8): G01N33/74G01N33/543
CPCG01N33/54326G01N33/743
Inventor 平璐孙成艳何浩会王香琪阚洪晶李娟
Owner DIRUI MEDICAL TECH CO LTD
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