ANS salt composite releaser and kit for detecting cortisol in blood

Abstract

The invention discloses an ANS salt composite releaser and a kit for detecting cortisol in blood. The kit comprises cortisol-antibody-coated magnetic beads, an acridinium-ester-labeled cortisol BSA (bovine serum albumin) composite, an ANS composite releaser and a cortisol calibration product. The ANS salt composite releaser converts the combined cortisol in the human blood sample into free cortisol, and the free cortisol content is detected by acridinium ester chemiluminescence. The ANS salt composite releaser can be combined with different glucocorticosteroid proteins, and can sufficiently release the combined cortisol in the blood. The acridinium ester chemiluminescence is flash luminescence, requires short time for luminescence, and can completely capture the photons generated by reaction. The acridinium ester is a synthetic substance, and has higher interference resistance to sample interfering materials. The acridinium ester label for detecting the free cortisol content is very sensitive, so that the total cortisol content detection in the blood sample is more accurate.

Description

technical field [0001] The invention relates to the technical field of immune analysis, in particular to an ANS salt compound releasing agent and a kit for detecting cortisol in blood. Background technique [0002] Cortisol, also known as "hydrocortisone", is a glucocorticoid produced by the adrenal gland in response to stress. When a person ingests cholesterol from food, under the action of the body's stress response, cholesterol will be converted into cortisol through the "glucocorticoid system" in the adrenal gland, and secreted into the blood in a free form. Cortisol has the function of regulating carbohydrate metabolism; immunosuppressive and anti-inflammatory effects; negative feedback regulation of corticotropin-releasing hormone secreted by hypothalamus and corticotropin secreted by pituitary. Therefore, by detecting the content of cortisol, the functional state of the adrenal gland can be diagnosed, and the functional state of the pituitary gland can be indirectly ...

AI Technical Summary

Problems solved by technology

[0010] Disadvantages: The luminescence of isoluminol and its derivatives is flash type, and its luminescence intensity mainly depends on the concentration of enzyme in the immune reaction. If no catalyst is used or the amount of catalyst is insufficient, the luminescent signal will become weak, and the amount of catalyst is difficult to control ;Secondly, isoluminol is interfered by hemolysis in the sample, which affects the determination of free cortisol content in blood

[0013] Insufficient: too sensitive to environmental factors and other non-specific reactions; too sensitive to metal chelates of metal ions, EDTA or other anticoagulants, preservatives; deposition of reaction by-products is also a potential source of interference; A sample of -7 is very sensitive ;O 2 Under the condition of adding voltage, it emits light under the condition of 620nm, and the electrochemiluminescence is interfered by the dissolved oxygen in the reaction system; the cleaning mechanism cleans the flow cell, and the flow cell is reused repeatedly, which has high requirements for cleaning

[0016] Insufficient: HRP is affected by heavy metal ions in the sample, temperature, NaN 3 Interference with other factors; the stability of the reagent is restricted by the stability of the reagent in the sample and the stability of HRP; the marker is a protein molecule, and the addition of the marker increases the steric hindrance of the antigen-antibody reaction

[0019] Insufficient: ALP is interfered by factors such as heavy metal ions and temperature in the sample; the stability of the reagent is restricted by the stability of the reagent in the sample and the stability of ALP; the marker is a protein molecule, and the addition of the marker increases the steric hindrance of the antigen-antibody reaction

[0020] As can be seen from the above, the existing in vitro diagnostic reagents have drawbacks in their clinical application.

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