Preparation method of high-solubility aflatoxins-removed rice bran protein

Abstract

The invention discloses a preparation method of high-solubility aflatoxins-removed rice bran protein. The preparation method comprises the following steps of degreasing aflatoxins polluted rice bran as a raw material, crushing the degreased aflatoxins polluted rice bran, screening the crushed aflatoxins polluted rice bran, mixing the screened aflatoxins polluted rice bran with a weak alkali solution, regulating pH to be 8.0-9.0, and performing stirring and mixing for 30-60 minutes so as to obtain a mixed solution; performing jetting and cooking treatment on the obtained mixed solution, then regulating pH to be 6.00, adding excess alpha-amylase and cellulase, performing hydrolysis at 40-60 DEG C to remove impurities including starch, fibers and the like, and performing centrifugal residual removal so as to obtain supernatant; and performing acid precipitation on the supernatant to produce white protein precipitate, performing enzyme deactivation, performing centrifugation, collecting protein precipitate, performing washing with water, and performing drying so as to obtain the rice bran protein. Through the adoption of the method disclosed by the invention, aflatoxins B1 in rice bran can be efficiently removed, the denaturation effect of strong basicity to protein and the bringing of a great quantity of sodium salt ions can be effectively avoided, the high-solubility aflatoxins-removed rice bran protein is mild and free from irritants, the utilization rate of the rice bran protein is high, and the prepared rice bran protein can be applied to clinical nutrients, weight-reducing health-care products, beverage making products and food auxiliary materials.

Description

Technical field [0001] The invention relates to a method for preparing rice bran protein, in particular to a method for preparing high-solubility aflatoxin rice bran protein. Background technique [0002] Rice bran is a by-product of rice processing, accounting for about 8% of the whole rice grain. It is the skin layer that is milled during the processing of brown rice into polished rice and a mixture of a small amount of rice germ and broken rice. It is twice as high as ordinary polished rice; my country’s annual total rice bran exceeds 18 million tons, which accounts for about 1 / 3 of the world’s total. It is rich in sources and has a high soluble protein content, accounting for about 70%, which is close to soybean protein and its nutritional value It is comparable to milk and eggs. Except for part of it being used as animal feed, most of it has not been developed and wasted. Therefore, the preparation of rice bran protein from rice bran has important practical significance for ...

AI Technical Summary

Problems solved by technology

CN103222535A discloses a method for preparing aflatoxin-free rice protein with improved functional properties, which uses microwave combined with alkali treatment to remove aflatoxin. The removal rate of aflatoxin in rice bran is not high when the above-mentioned detoxification method is applied (Rice bran product Aspergillus flavus B 1 ≥10.0μg / kg), and the effect of improving the solubility and quality of rice bran protein is not ideal, so it is difficult to apply in actual industrial production

In addition, because the nutrients in rice bran are combined with its cell wall, the solubility is poor and it is difficult to be utilized. However, when using the traditional "alkali-soluble acid precipitation" protein preparation process or the above-mentioned microwave-assisted treatment technology, the rice bran protein product is prepared. Aflatoxin AFB 1 The degradation rate only reaches 40.65%~49.52%, far exceeding the national standard limit of 10μg / kg. The high pH used in "alkali dissolution" leads to protein denaturation and even toxic substances, and "acid precipitation" brings in too much inorganic salt and desalination It will cause protein loss. The rice bran protein prepared by this method, because the rice bran protein itself contains more disulfide bonds, affects the solubility in aqueous solution, resulting in low extraction of rice bran protein and poor nutritional quality, and the cost is high. It is difficult to Realize industrial level production

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