Composite type synbiotic microecological preparation for rabbits and preparation method of composite type synbiotic microecological preparation
A kind of micro-ecological preparation, compound technology, applied in application, animal husbandry, additional food elements, etc., can solve the problems of low absorption and utilization rate, poor application effect, etc., and achieve the effect of promoting animal growth, promoting colonization, and improving production performance
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[0039] Another object of the present invention is to provide a preparation method of the compound synbiotic probiotics for rabbits, the preparation method comprising the following steps:
[0040] Firstly, the intestinal probiotics are isolated and screened out in a targeted manner. By comparing the changes in the number of viable probiotics in the simulated intestinal environment, the best strains are screened out, and the content of various oligosaccharides is determined to select the best probiotics. The amount of addition is to mix probiotics and oligosaccharides suitable for the stomach and intestines of rabbits to make a compound synbiotic microecological preparation.
[0041] Further, the preparation method of the composite synbiotic probiotics for rabbits specifically includes:
[0042] Step 1. Isolate and screen Enterococcus faecalis (Streptococcus faecalis), Bacillus subtilis and Bifidobacterium from the soil in the rabbit farm, feed the feed tank, and the gastrointes...
Embodiment 1
[0061] Example 1: Preliminary screening of Bacillus probiotics from the soil near the farm.
[0062] The first step: collection of soil samples containing Bacillus. Select the soil near the large farm as the collection destination, randomly select 3-5 plots, take 20-50g of original soil from each plot, put it into a sterilized ziplock bag, bring it back to the laboratory and place it in a 4°C refrigerator Save for later use;
[0063] The second step: the isolation of bacillus. Under sterile conditions, the soil sample was added to the enriched culture solution to form colonies, and then the bacteria were inoculated into sterile saline to make a bacterial suspension, and then diluted into different concentration gradients by a 10-serial decreasing method. Count the colonies on the plates of different solubility gradient samples, and make 3 parallels for each dilution, and put the resulting plates into an incubator for 24-72 hours;
[0064] The third step: separation and puri...
Embodiment 2
[0066] Example 2: Preliminary screening of bifidobacteria probiotics from animal intestines.
[0067] The first step: collection of samples containing probiotics. Select each large-scale slaughterhouse as the sample collection destination, randomly cut the intestinal tissues of different healthy freshly killed animals, put them into sterilized ziplock bags, and quickly bring them back to the laboratory and store them in a 4°C refrigerator for later use;
[0068] The second step: enrichment and separation of probiotics. Under sterile conditions, the inner surface substances of the intestinal tissue samples were added to the enriched culture medium for culture to form colonies, and then the bacteria were inoculated into sterile physiological saline to make a bacterial suspension, and then diluted by 10 serial decremental method Make different concentration gradients, count different solubility gradient samples on plate colonies, do 3 parallels for each dilution, and put the res...
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