Composite type synbiotic microecological preparation for rabbits and preparation method of composite type synbiotic microecological preparation

A kind of micro-ecological preparation, compound technology, applied in application, animal husbandry, additional food elements, etc., can solve the problems of low absorption and utilization rate, poor application effect, etc., and achieve the effect of promoting animal growth, promoting colonization, and improving production performance

Inactive Publication Date: 2017-03-22
SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The object of the present invention is to provide a compound synbiotic microecological preparation for rabbits and its preparation method, aiming to solve the problems of low absorption and utilization rate and poor application effect of the current synbiotic probiotic preparation for animals

Method used

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  • Composite type synbiotic microecological preparation for rabbits and preparation method of composite type synbiotic microecological preparation

Examples

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preparation example Construction

[0039] Another object of the present invention is to provide a preparation method of the compound synbiotic probiotics for rabbits, the preparation method comprising the following steps:

[0040] Firstly, the intestinal probiotics are isolated and screened out in a targeted manner. By comparing the changes in the number of viable probiotics in the simulated intestinal environment, the best strains are screened out, and the content of various oligosaccharides is determined to select the best probiotics. The amount of addition is to mix probiotics and oligosaccharides suitable for the stomach and intestines of rabbits to make a compound synbiotic microecological preparation.

[0041] Further, the preparation method of the composite synbiotic probiotics for rabbits specifically includes:

[0042] Step 1. Isolate and screen Enterococcus faecalis (Streptococcus faecalis), Bacillus subtilis and Bifidobacterium from the soil in the rabbit farm, feed the feed tank, and the gastrointes...

Embodiment 1

[0061] Example 1: Preliminary screening of Bacillus probiotics from the soil near the farm.

[0062] The first step: collection of soil samples containing Bacillus. Select the soil near the large farm as the collection destination, randomly select 3-5 plots, take 20-50g of original soil from each plot, put it into a sterilized ziplock bag, bring it back to the laboratory and place it in a 4°C refrigerator Save for later use;

[0063] The second step: the isolation of bacillus. Under sterile conditions, the soil sample was added to the enriched culture solution to form colonies, and then the bacteria were inoculated into sterile saline to make a bacterial suspension, and then diluted into different concentration gradients by a 10-serial decreasing method. Count the colonies on the plates of different solubility gradient samples, and make 3 parallels for each dilution, and put the resulting plates into an incubator for 24-72 hours;

[0064] The third step: separation and puri...

Embodiment 2

[0066] Example 2: Preliminary screening of bifidobacteria probiotics from animal intestines.

[0067] The first step: collection of samples containing probiotics. Select each large-scale slaughterhouse as the sample collection destination, randomly cut the intestinal tissues of different healthy freshly killed animals, put them into sterilized ziplock bags, and quickly bring them back to the laboratory and store them in a 4°C refrigerator for later use;

[0068] The second step: enrichment and separation of probiotics. Under sterile conditions, the inner surface substances of the intestinal tissue samples were added to the enriched culture medium for culture to form colonies, and then the bacteria were inoculated into sterile physiological saline to make a bacterial suspension, and then diluted by 10 serial decremental method Make different concentration gradients, count different solubility gradient samples on plate colonies, do 3 parallels for each dilution, and put the res...

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Abstract

The invention discloses a composite type synbiotic microecological preparation for rabbits and a preparation method of the composite type synbiotic microecological preparation. The preparation method comprises the following steps of firstly screening out intestinal tract probiotics under different environments, comparing the functions and the biological characteristics of the screened-out intestinal tract probiotics, comparing the viable count changes of probiotics under a simulated intestinal tract environment, screening out optimal bacterial strains, determining the content of various oligosaccharides, selecting optimal addition quantity, and mixing the intestinal tract probiotics with the oligosaccharides so as to obtain the composite type synbiotic microecological preparation. According to the composite type synbiotic microecological preparation and the preparation method thereof disclosed by the invention, the field planting of exogenous probiotics in the intestinal tracts of the rabbits is promoted, the growth and the propagation of various probiotics are selectively stimulated, the influence of edible animal antibiotics on rabbit meat quality is reduced, the conversion rate of a feed can also be increased, the meat quality can also be improved, the safety quality of foods can also be improved, and the food safety and quality problems that medicine residues exist, medicine resistance is produced, the microecology balance of the intestinal tracts of animals is destroyed and the quality of meat products is reduced caused by conventional antibiotics are solved.

Description

technical field [0001] The invention belongs to the field of biological preparations, in particular to a composite synbiotic probiotic preparation for rabbits and a preparation method thereof. Background technique [0002] At present, there are few studies on the application of synbiotics microecological preparations in animal production in my country, and the use of microecological preparations in animal food additives in animal husbandry is still relatively small. People are vague about the application of probiotics, that is, confusion is targeted Sexually added objects, resulting in animal growth that is often less than expected. The emergence of probiotics is due to the improvement of people's living standards, and people gradually realize that antibiotics added to feed for a long time can cause harm to human health through the food chain. The research and application of microecological preparations overcomes the various disadvantages caused by the long-term use of antib...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23K50/50A23K10/18A23K20/163A23K20/105
Inventor 刘君刘华康王晗陈静罗雁刘永明张薇
Owner SICHUAN UNIVERSITY OF SCIENCE AND ENGINEERING
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