A kind of preparation method and application of drug-loaded porous phbv grafted dopamine microspheres
A technology of dopamine microspheres and porous microspheres, applied in medical science, prosthesis, etc., can solve the problems of poor biological activity of PHBV, and achieve the effect of good bacteriostatic effect, improved utilization rate, and mild reaction conditions.
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0021] The preparation method of the drug-loaded porous PHBV grafted dopamine microspheres of the present embodiment, the steps are as follows:
[0022] (1) Put 10g of PHBV powder into an oxygen plasma processor to evacuate to 7Pa, then pass ammonia gas to 20Pa, treat at 200W for 5min, then clean and dry the treated PHBV material to obtain carboxylated PHBV (PHBV-COOH);
[0023] (2) Dry the PHBV-COOH at 50°C for 8 hours in vacuum, then dissolve 1g (0.015mol) of anhydrous PHBV-COOH in 100mL of dichloromethane solution, and then add 4.75g (0.03mol) of N,N' - Carbonyldiimidazole, activate the carboxyl group for 1 hour, then add 1.64g of dopamine (0.015mol) into the PHBV-COOH solution, stir and react for 8 hours to obtain PHBV-DOPA, after the reaction, precipitate PHBV-DOPA, wash and dry;
[0024] (3) Dissolve 10g of PHBV-DOPA in 100mL of dichloromethane solution, then slowly inject it into liquid nitrogen at a rate of 30mL / h using a micro-syringe pump, and at the same time apply...
Embodiment 2
[0027] The preparation method of the drug-loaded porous PHBV grafted dopamine microspheres of the present embodiment, the steps are as follows:
[0028] (1) Put 10g of PHBV powder into an oxygen plasma processor to evacuate to 7Pa, then pass ammonia gas to 30Pa, treat at 300W for 40min, then clean and dry the treated PHBV material to obtain carboxylated PHBV (PHBV-COOH);
[0029] (2) Dissolve 1g (0.015mol) of anhydrous PHBV-COOH in 100mL of dichloromethane solution, then add 12.15g (0.075mol) of N,N'-carbonyldiimidazole to activate the carboxyl group for 1h, then add 3.28g (0.03mol) dopamine was added to the PHBV-COOH solution, stirred and reacted for 8 hours to obtain PHBV-DOPA, after the reaction was completed, PHBV-DOPA was precipitated, washed and dried;
[0030] (3) Dissolve 10g of PHBV-DOPA in 100mL of dichloromethane solution, then slowly inject it into liquid nitrogen at a rate of 60mL / h using a micro-syringe pump, and at the same time apply a voltage of 20kV to the n...
Embodiment 3
[0033] The preparation method of the drug-loaded porous PHBV grafted dopamine microspheres of the present embodiment, the steps are as follows:
[0034] (1) Put 10g of PHBV powder into an oxygen plasma processor to evacuate to 7Pa, then pass ammonia gas to 20Pa, treat at 200W for 25min, then clean and dry the treated PHBV material to obtain carboxylated PHBV;
[0035] (2) Dissolve 1g (0.015mol) of anhydrous PHBV-COOH in 100mL of dichloromethane solution, then add 9.50g (0.06mol) of N,N'-carbonyldiimidazole to activate the carboxyl group for 1h, and then add 1.64g (0.015mol) dopamine was added to the PHBV-COOH solution, stirred and reacted for 8 hours to obtain PHBV-DOPA, after the reaction was completed, the PHBV-DOPA was precipitated, washed and dried;
[0036] (3) Dissolve 10g of PHBV-DOPA in 100mL of dichloromethane solution, then slowly inject it into liquid nitrogen at a rate of 40mL / h using a micro-syringe pump, and at the same time apply a voltage of 10kV to the needle...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com