A kind of preparation method and application of drug-loaded porous phbv grafted dopamine microspheres

A technology of dopamine microspheres and porous microspheres, applied in medical science, prosthesis, etc., can solve the problems of poor biological activity of PHBV, and achieve the effect of good bacteriostatic effect, improved utilization rate, and mild reaction conditions.

Inactive Publication Date: 2018-04-10
乐土佑嘉(深圳)医药科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to provide a preparation method of drug-loaded porous PHBV-grafted dopamine microspheres, mainly to solve the poor biological activity of PHBV and to make up for some deficiencies in the preparation method of the current PHBV porous drug-loaded microspheres

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] The preparation method of the drug-loaded porous PHBV grafted dopamine microspheres of the present embodiment, the steps are as follows:

[0022] (1) Put 10g of PHBV powder into an oxygen plasma processor to evacuate to 7Pa, then pass ammonia gas to 20Pa, treat at 200W for 5min, then clean and dry the treated PHBV material to obtain carboxylated PHBV (PHBV-COOH);

[0023] (2) Dry the PHBV-COOH at 50°C for 8 hours in vacuum, then dissolve 1g (0.015mol) of anhydrous PHBV-COOH in 100mL of dichloromethane solution, and then add 4.75g (0.03mol) of N,N' - Carbonyldiimidazole, activate the carboxyl group for 1 hour, then add 1.64g of dopamine (0.015mol) into the PHBV-COOH solution, stir and react for 8 hours to obtain PHBV-DOPA, after the reaction, precipitate PHBV-DOPA, wash and dry;

[0024] (3) Dissolve 10g of PHBV-DOPA in 100mL of dichloromethane solution, then slowly inject it into liquid nitrogen at a rate of 30mL / h using a micro-syringe pump, and at the same time apply...

Embodiment 2

[0027] The preparation method of the drug-loaded porous PHBV grafted dopamine microspheres of the present embodiment, the steps are as follows:

[0028] (1) Put 10g of PHBV powder into an oxygen plasma processor to evacuate to 7Pa, then pass ammonia gas to 30Pa, treat at 300W for 40min, then clean and dry the treated PHBV material to obtain carboxylated PHBV (PHBV-COOH);

[0029] (2) Dissolve 1g (0.015mol) of anhydrous PHBV-COOH in 100mL of dichloromethane solution, then add 12.15g (0.075mol) of N,N'-carbonyldiimidazole to activate the carboxyl group for 1h, then add 3.28g (0.03mol) dopamine was added to the PHBV-COOH solution, stirred and reacted for 8 hours to obtain PHBV-DOPA, after the reaction was completed, PHBV-DOPA was precipitated, washed and dried;

[0030] (3) Dissolve 10g of PHBV-DOPA in 100mL of dichloromethane solution, then slowly inject it into liquid nitrogen at a rate of 60mL / h using a micro-syringe pump, and at the same time apply a voltage of 20kV to the n...

Embodiment 3

[0033] The preparation method of the drug-loaded porous PHBV grafted dopamine microspheres of the present embodiment, the steps are as follows:

[0034] (1) Put 10g of PHBV powder into an oxygen plasma processor to evacuate to 7Pa, then pass ammonia gas to 20Pa, treat at 200W for 25min, then clean and dry the treated PHBV material to obtain carboxylated PHBV;

[0035] (2) Dissolve 1g (0.015mol) of anhydrous PHBV-COOH in 100mL of dichloromethane solution, then add 9.50g (0.06mol) of N,N'-carbonyldiimidazole to activate the carboxyl group for 1h, and then add 1.64g (0.015mol) dopamine was added to the PHBV-COOH solution, stirred and reacted for 8 hours to obtain PHBV-DOPA, after the reaction was completed, the PHBV-DOPA was precipitated, washed and dried;

[0036] (3) Dissolve 10g of PHBV-DOPA in 100mL of dichloromethane solution, then slowly inject it into liquid nitrogen at a rate of 40mL / h using a micro-syringe pump, and at the same time apply a voltage of 10kV to the needle...

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Abstract

The invention discloses a preparation method and application of drug-loading porous PHBV grafted dopamine microparticles. The preparation method comprises the following steps that the surface of PHBV is subjected to carboxylation through the oxygen plasma technology, and PHBV-COOH is obtained; dopamine is coupled to the surface of the PHBV-COOH, and dopamine-decorated PHBV, namely PHBV-DOPA is obtained; PHBV-DOPA microparticles are prepared through the electrostatic drop condensation method, and then PHBV-DOPA porous microparticles are prepared through the freeze-drying method; and antibacterial drugs are adsorbed in pores of the PHBV-DOPA porous microparticles through the freeze-drying method, and the drug-loading porous PHBV-DOPA microparticles are obtained. By the adoption of the preparation method and application, the biological activity of the PHBV is effectively improved, and meanwhile, the antimicrobial property of the PHBV is improved; and in addition, the preparation technique is simple, reaction conditions are mild, and the application range of the PHBV is effectively broadened.

Description

technical field [0001] The invention relates to a preparation method and application of a drug carrier and a tissue engineering material, belonging to the field of medical polymers, in particular to a new preparation method and application of drug-loaded porous PHBV grafted dopamine microspheres. Background technique [0002] Polyhydroxybutyrate valeric acid copolyester (PHBV) is a water-insoluble polymer stored in the cells of bacteria under the condition of unbalanced growth. It is biodegradable, non-antigenic and non-toxic. It has no carcinogenicity and good biocompatibility, and has received extensive attention in recent years. [0003] At the same time, PHBV has broad application prospects in the field of medicine, for example, it can be used as a substrate for surgical sutures, a substitute for bones and blood vessels, and as a carrier for sustained release of drugs. In view of the fact that PHBV has no side effects on the human body, the use of PHBV as a sustained-re...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C08G63/91C08J9/40C08J9/28C08J3/12A61L27/18A61L27/50A61L27/54A61L27/56
CPCA61L27/18A61L27/50A61L27/54A61L27/56A61L2300/404A61L2300/412C08G63/912C08J3/12C08J9/28C08J9/40C08J2201/0482C08J2367/04C08L67/04
Inventor 王延伟范利丹迟长龙徐茜张浩于翔魏媛杨秀琴
Owner 乐土佑嘉(深圳)医药科技有限公司
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