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Chinese toon Alternaria mali 56-50 and its secondary metabolite, preparation method and use

A technology for secondary metabolites and endophytic fungi, applied in the field of microorganisms, can solve problems such as difficult treatment, and achieve the effect of inhibiting bacterial activity, low cost, and good inhibitory effect

Active Publication Date: 2017-03-22
HENAN ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to overcome the existing difficulties in the treatment of Pseudomonas aeruginosa and / or Proteus infection, the object of the present invention is to provide a Toona sinensis endophytic fungus 56-50 and its secondary metabolites, preparation method and application

Method used

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  • Chinese toon Alternaria mali 56-50 and its secondary metabolite, preparation method and use
  • Chinese toon Alternaria mali 56-50 and its secondary metabolite, preparation method and use
  • Chinese toon Alternaria mali 56-50 and its secondary metabolite, preparation method and use

Examples

Experimental program
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Effect test

Embodiment 1

[0043] 1. Isolation and Screening of Toona sinensis Endophytic Fungi

[0044] Under aseptic operating conditions, the Chinese toon stems were soaked in 75v% ethanol, 1wt% sodium hypochlorite of available chlorine, and 75v% ethanol for 1 min each, then rinsed with sterile water for 3 times, and dried with sterile absorbent paper; Cut it into 8mm pieces, put it on the PDA plate medium, 4 roots per plate, culture at 28°C until the end of the stem grows hyphae, transfer the mycelia to a new PDA plate medium for streaking culture, 5d After isolation and purification, a single colony was obtained, and then transferred to a PDA slant medium test tube. After 5 days of constant temperature cultivation at 28°C, the endophytic fungus 56-50 of Toona sinensis was obtained, stored in a refrigerator at 4°C, and used for later use. Preserved in China General Microorganism Culture Collection Management Center, preservation number: CGMCC No.12980.

[0045] 2. Identification of Toona sinensis e...

Embodiment 2

[0079] It is basically the same as Example 1, except that this example completes the fermentation, preparation and antibacterial activity determination of the secondary metabolites of the strain according to the following main steps.

[0080] 1-2 steps are the same as in Example 1.

[0081] 3. Small-scale fermentation of the strain:

[0082] Inoculate the Toona sinensis endophytic fungus 56-50 stored in the refrigerator at 4°C on the PDA plate medium, activate and culture in the incubator at 28°C for 4 days, then inoculate into the Erlenmeyer flask containing 200mL PDA liquid fermentation medium, and let it stand at room temperature Fermentation culture 7d.

[0083] 4. Preparation of strain secondary metabolites:

[0084] After the fermentation of the strains is completed, the fermentation culture liquid of the strain 56-50 is first crushed with a homogenizer mixer, and then filtered through a Buchner funnel to obtain the fermentation liquid and mycelia; the fermentation liq...

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Abstract

The invention belongs to the technical field of microorganisms and discloses Chinese toon Alternaria mali 56-50. The Chinese toon Alternaria mali 56-50 is Alternaria mali in Alternaria of dematiaceous hyphomycetes of Hyphomycetales of Hyphomycetes of Fungi imperfecti, is preserved in the China general microbiological culture collection center in the institute of microbiology on No.3, Beichen West Road, Chaoyang District, Beijing, on September 30, 2016 and has an accession number of CGMCC No. 12980. The Chinese toon Alternaria mali is isolated from the stem of Chinese toon at first, and the secondary metabolites of the Chinese toon Alternaria mali have a good inhibitory effect on Pseudomonas aeruginosa and proteus. A method for preparing the secondary metabolite of the Chinese toon Alternaria mali 56-50 is simple and realizes a low cost.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Chinese toon endophytic fungus 56-50 and its secondary metabolites, preparation method and application. Background technique [0002] Pseudomonas aeruginosa is an opportunistic pathogen and one of the main pathogens of nosocomial infection. Patients suffering from metabolic diseases, blood diseases and malignant tumors, as well as patients after surgery or certain treatments are susceptible to infection with this bacteria. Infection lesions caused by this bacteria can lead to hematogenous dissemination, resulting in bacteremia and sepsis. Infection with Pseudomonas aeruginosa after burns can cause death. [0003] Proteus is also an opportunistic pathogen, which is widely distributed in nature, such as soil, water, garbage, spoilage organic matter, and human or animal intestines, and can cause various infections. Common infections include respiratory tract ...

Claims

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Application Information

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IPC IPC(8): C12N1/14C12P1/02A61K36/06A61P31/04C12R1/645
CPCA61K36/06C12P1/02C12N1/145C12R2001/645
Inventor 王赵改王晓敏赵洪源史冠莹赵守涣杨慧张乐
Owner HENAN ACAD OF AGRI SCI
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