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Preparation method of fungal inoculant for improving soil heavy metal pollution

A fungal inoculum and a technology for improving soil are applied in the field of preparation of fungal inoculants to achieve the effects of saving fermentation time and cost, cheap materials and high utility.

Active Publication Date: 2017-03-22
周宇
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] In order to make up for the deficiencies of the prior art, the present invention provides a method for preparing a fungal agent that is scientifically prepared, has low cost, and improves soil heavy metal pollution. The agent prepared by the preparation method is applied to high-chromium and high-lead soils, The fertilizer effect is remarkable, which can effectively avoid the heavy metal contamination of plant roots in the above soil, degrade heavy metals, improve soil quality, promote plant growth, and solve the problems existing in the prior art

Method used

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  • Preparation method of fungal inoculant for improving soil heavy metal pollution
  • Preparation method of fungal inoculant for improving soil heavy metal pollution
  • Preparation method of fungal inoculant for improving soil heavy metal pollution

Examples

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Effect test

Embodiment 1

[0029] The preparation method of the fungal agent for improving soil heavy metal pollution adopts the following steps:

[0030] (1) Pick the edge hyphae of the slanted strains of Boletus cerevisiae, Boletus deliciosa and Boletus red versicolor, respectively, and inoculate them in PDA solid medium, culture in dark at 28°C for 48 hours to obtain activated bacteria kind;

[0031] (2) get step (1) through the mycelium culture medium of each strain of activated culture, be cut into 1cm mycelium fritter respectively, for subsequent use;

[0032] (3) Under aseptic conditions, transfer the mycelium fragments of Lactobacillus brown ring obtained in step (2) to the liquid culture medium of Lactobacillus brown ring, and shake at 120r / min on a shaking table under the condition of 28°C in the dark Cultivate for 5 days to obtain the seed liquid of Boletus cerevisiae;

[0033] Under sterile conditions, transfer the boletus velvet cap boletus mycelium fragment obtained in step (2) to the bo...

Embodiment 2

[0051] The preparation method of the fungal agent for improving soil heavy metal pollution adopts the following steps:

[0052] (1) Pick the edge hyphae of the slanted strains of Boletus cerevisiae, Boletus deliciosa and Boletus red versicolor, respectively, and inoculate them in PDA solid medium, culture in dark at 28°C for 48 hours to obtain activated bacteria kind;

[0053] (2) get step (1) through the mycelium culture medium of each strain of activated culture, be cut into 2cm mycelium fritters respectively, for subsequent use;

[0054] (3) Under aseptic conditions, transfer the mycelium fragments of Lactobacillus brown ring obtained in step (2) to the liquid culture medium of Lactobacillus brown ring, and shake at 120r / min on a shaking table at 26°C in the dark. Cultivate for 6 days to obtain the seed liquid of Boletus cerevisiae;

[0055] Under sterile conditions, transfer the small pieces of boletus erythrosum mycelia obtained in step (2) to the liquid culture medium ...

Embodiment 3

[0073] The preparation method of the fungal agent for improving soil heavy metal pollution adopts the following steps:

[0074] (1) Pick the edge hyphae of the slanted strains of Boletus cerevisiae, Boletus deliciosa and Boletus red versicolor, respectively, and inoculate them in PDA solid medium, culture in dark at 28°C for 48 hours to obtain activated bacteria kind;

[0075] (2) get step (1) through the mycelium culture medium of each strain of activated culture, be cut into 1cm mycelium fritter respectively, for subsequent use;

[0076] (3) Under aseptic conditions, transfer the mycelium fragments of Lactobacillus brown ring obtained in step (2) to the liquid culture medium of Lactobacillus brown ring, and shake at 120r / min on a shaking table at 30°C in the dark. Cultivate for 5 days to obtain the seed liquid of Boletus cerevisiae;

[0077] Under sterile conditions, transfer the small pieces of boletus rhododendron mycelia obtained in step (2) to the liquid culture medium...

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Abstract

The invention belongs to the technical field of microbial inoculants, and especially relates to a preparation method of a fungal inoculant for improving soil heavy metal pollution. The preparation method of the fungal inoculant for improving soil heavy metal pollution comprises the following steps: 1, activating bacterial fungal seeds; 2, cutting mycelia to form small blocks; 3, transferring the small mycelia blocks into a corresponding liquid medium, and carrying out shaking table oscillation dark culture to obtain a seed liquid of all the fungi; 4, uniformly mixing inoculant carriers according to a certain weight ratio, crushing and sieving the mixed inoculant carriers to obtain powder, and carry out high temperature sterilization to obtain an inoculant carrier mixture; 5, adding the inoculant carrier mixture and sterile water into a fermentation tank in order to obtain a mixed medium; , inoculating the mixed medium with the seed liquid of all the fungi, and carrying out dark culture to obtain a fermentation fungus liquid; and 7, filtering the fermentation fungus liquid, drying the filtered liquid, crushing the dried liquid, and packaging the crushed dried liquid to obtain the fungal inoculant. The preparation method of the fungal inoculant for improving soil heavy metal pollution has the advantages of scientific property and low cost, and the prepared fungal inoculant has a substantial fertilizer effect, can effectively avoid heavy metal invasion of plant roots in soil, and improves the quality of the soil.

Description

[0001] (1) Technical field [0002] The invention belongs to the technical field of microbial inoculum, and in particular relates to a preparation method of a fungal inoculum for improving soil heavy metal pollution. [0003] (2) Background technology [0004] The accumulation of heavy metals in the soil leads to changes in soil properties, directly affecting the supply of soil nutrients and fertility characteristics. The mineralization of nitrogen in heavy metal-polluted soils is significantly reduced, and the soil nitrogen supply capacity is reduced; the absorption and fixation of phosphorus in heavy metal-polluted soils is enhanced, and the availability of soil phosphorus is reduced; Decrease, the water-soluble potassium content rises, accelerates the loss of potassium, and ultimately affects the maintenance and supply of nitrogen, phosphorus, and potassium in the soil. [0005] The hyphae of ectomycorrhizal fungi can form a tightly intertwined mantle on the surface of plan...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C09K17/14C12R1/645
CPCC09K17/14C12N1/14
Inventor 周宇
Owner 周宇
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