Production method of high-content mannan yeast culture

A technology of yeast culture and mannan, applied in the field of bioengineering, to achieve high fermentation efficiency, good process stability, and no mold pollution

Active Publication Date: 2019-10-18
河北斐默特生物科技有限公司 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0006] Although mannan is a key functional factor of yeast culture, there are few reports in the literature related to yeast culture production technology. The current technology research and development of yeast culture mainly focuses on the selection of cheap substrates. In the final analysis, it is mainly to reduce costs. , turning waste into wealth, such as Chinese patent (application number: 201410076270.0) has reported the preparation of yeast culture using rice distiller's grains as a substrate; Mobile fermentation of yeast culture; in addition, Chinese patent (application number: 201510578518.8) reported the fermentation production of yeast culture with mixed dross as raw material

Method used

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preparation example Construction

[0032] Step a, preparation of Saccharomyces cerevisiae seed liquid: pick 1 to 2 rings of Saccharomyces cerevisiae strains preserved on the inclined plane, inoculate them into the seed medium, and vibrate and culture them on a shaking table to obtain Saccharomyces cerevisiae seed liquid;

[0033] Step b, liquid high-density culture of Saccharomyces cerevisiae: inoculate the Saccharomyces cerevisiae seed liquid into a reaction tank equipped with a liquid fermentation medium, and cultivate it in a medium temperature and weak acid environment;

[0034] Step c, culture of Saccharomyces cerevisiae with high content of mannan at low temperature and hydrogen peroxide stress: reduce the reaction temperature of the reaction tank, and at the same time, add 15% to 25% mass concentration of hydrogen peroxide into the reaction tank at a constant speed until the final concentration of hydrogen peroxide in the reaction tank reach 6-10mmol / L, and then continue to cultivate for several hours to ...

Embodiment 1

[0049] A production method of high-content mannan yeast culture, comprising the following steps:

[0050] Step a, preparation of yeast seed liquid: Pick 2 rings of Saccharomyces cerevisiae (CICC1355) strains preserved on a slant, inoculate them into the seed culture medium, and culture on a shaker at 30° C. and 200 rpm for 18 hours.

[0051] Step b. Liquid high-density culture of Saccharomyces cerevisiae: inoculate the seed culture solution into a 1000-liter reaction tank equipped with a liquid fermentation medium, the temperature in the tank is 30°C, the pH is controlled within the range of 5.0 to 6.0, the stirring speed is 200 rpm, and the ventilation rate is 1.0vvm culture 20h.

[0052] Step c, culture of Saccharomyces cerevisiae with high content of mannan at low temperature and hydrogen peroxide stress: lower the temperature of the fermentation system in step b to 24°C, and at the same time, add 20% mass concentration of hydrogen peroxide into the reaction tank at a const...

Embodiment 2

[0061] Step a, preparation of Saccharomyces cerevisiae seed liquid: pick 2 rings of Saccharomyces cerevisiae strains preserved on the slant, inoculate them into the seed medium, and shake and culture at 32°C and 250rpm for 16 hours;

[0062] Step b. Liquid high-density culture of Saccharomyces cerevisiae: Inoculate the seed culture solution into a reaction tank equipped with a liquid fermentation medium, the temperature in the tank is 32°C, the pH is controlled within the range of 5.0 to 6.0, the stirring speed is 150rpm, and the ventilation rate is 0.75vvm Cultivate for 18h.

[0063] Step c, culture of Saccharomyces cerevisiae with high content of mannan at low temperature and hydrogen peroxide stress: lower the temperature of the fermentation system in step b to 26°C, and at the same time, add 15% hydrogen peroxide into the reaction tank at a constant speed through a peristaltic pump until the final concentration reaches 8mmol / L, continue to culture for 4 hours.

[0064] St...

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Abstract

The invention discloses a production method for a high-content mannan yeast culture and belongs to the technical field of bioengineering. The production method comprises the steps that a wine making yeast seed solution is inoculated into a liquid fermentation culture medium at first, and aerobic culturing is conducted for 18-20 h at the temperature of 30 DEG C-32 DEG C, so that a thallus reaches the high-density level; then the culturing temperature is lowered to 24 DEG C-26 DEG C, hydrogen peroxide is added into a fermentation system at the same time till the final concentration is 6-10 mmol / L, and culturing is continuously conducted; and a fermentation culture solution and a solid culture medium are mixed at the weight ratio of 3:6-3:8 and are transferred into a solid fermentation box to be subjected to anaerobic culturing for 24-28 hours, then low-temperature drying is conducted, and the high-content mannan yeast culture can be obtained through smashing. The mannan content of the high-content mannan yeast culture is 4.0%-4.5%, and the water-soluble protein is 7%-9%. According to the high-content mannan yeast culture, the cow milk yield can be significantly increased by 1-2 kg / (head.day), the number of cow somatic cells is effectively decreased, the cow health condition is significantly improved, and the high-content mannan yeast culture is good for the drinker health.

Description

technical field [0001] The invention relates to a production method of a high-content mannan yeast culture, belonging to the technical field of bioengineering. Background technique [0002] Yeast culture usually refers to the compound culture obtained by full fermentation of Saccharomyces cerevisiae under specific technological and nutritional conditions. The culture mainly consists of three parts: yeast cells, yeast metabolites, and denatured nutritional substrates. Among these components Rich in mannan, dextran, amino acids, B vitamins, nucleic acids and other active ingredients and some unknown functional factors. As a functional feed material, yeast culture has significant effects of disease resistance and production increase, and can provide nutrients to microorganisms in the digestive tract of animals, thereby stimulating their metabolic activity and stabilizing the micro-ecological environment in the body. Compared with antibiotic additives, it has Green and natural,...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/18A23K10/16A23L31/10C12R1/865
Inventor 刘金龙王卫正刘志勤郭忠海张晓腾王增立冯志强褚健
Owner 河北斐默特生物科技有限公司
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