A compound probiotic for regulating blood sugar and preparation method thereof
A compound probiotic and blood sugar technology, which is applied in the direction of medical preparations containing active ingredients, bacteria, drug combinations, etc., can solve the problems of no ideal method, etc., and achieve the effect of simple process, wide source of raw materials, and easy operation of the process
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Embodiment 1
[0040] A compound prebiotic (chewable tablet or capsule) for regulating blood sugar, which is made of the following raw materials in parts by weight:
[0041]
[0042]
[0043] The additive is composed of any one of milk essence, jujube essence, ethyl maltol, guar gum, and sucralose, or any mixture of two to five of them.
[0044] The Gordona terrae sq-5 (Gordona terrae sq-5) CCTCC NO: M2012390.
Embodiment 2
[0046] A preparation method of a compound prebiotic (chewable tablet or capsule) for regulating blood sugar, the steps are:
[0047] A, strain culture: slant seed culture medium Cha's medium, freeze-dried seeds Gordonia terrestris sq-5 (patent No.: ZL2013 1 0016059.5) was inoculated on the slant of eggplant bottle, 28 or 29 or 30 or 31 or Incubate at 32°C for 3 days. Check that there is no miscellaneous bacteria, the growth is robust and uniform, and transfer to the inoculation bottle with sterile water.
[0048] B. Seed tank cultivation: Transfer the seed from the inoculation bottle described in A to the seed tank, cultivate at 28 or 29 or 30 or 31 or 32°C, and ventilate at 1:0.6 or 0.7 or 0.8, add bubble enemy automatically, and cultivate for 30 Or 32 or 34 or 35h, take a sample for microscopic inspection, and reserve after passing the test.
[0049] C. Fermentation tank culture: inject the seed liquid of the seed tank in the above-mentioned B into the fermenter with an in...
Embodiment 3
[0055] The impact of Gordonia terrestris sq-5 whole cell powder on the immune function of mice, the steps are:
[0056] The experimental animals were 62 Kunming male mice with a body weight of 20±2g. They were randomly divided into four groups, 20 in the distilled water+feed group (CK), and 0.0001g, 0.001g, 0.01Gordonia terrestris powder were added to the feed respectively. g, 14 animals each, were fed continuously for 14 days. On the 15th day, spleen lymphocytes were transformed to induce the production of IL-2, TNF and thymus weight. The results are shown in Table 1 and Table 2.
[0057] Table 1 Effect of Gordonia terrestris sq-5 bacterial powder on transformation of splenic lymphocytes
[0058] group
Number of mice N
normal saline
conA-induced
CK
20
3800±437
11258±1713
0.0001
14
8343±402
18307±3143
0.001
14
11286±2317
24532±4315
0.01
14
12688±1302
29563±2811
[0059] The ratio of the thr...
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