Unlock instant, AI-driven research and patent intelligence for your innovation.

Method for determining flavone components in tea leaf by using HPLC

A technology of middle flavonoids and ketones, applied in the field of tea detection, can solve the problems of simultaneous determination of no ingredients, and achieve the effects of broadening the scope of use, being environmentally friendly, and reducing extraction time.

Inactive Publication Date: 2017-04-19
MENGHAI TEA IND
View PDF4 Cites 4 Cited by
  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0007] At present, most of the flavonoids in tea leaves detected by HPLC are kaempferin, quercetin, myricetin or quercitrin, but kaempferenin, quercetin, myricetin, and their glycosides have not yet been included. Simultaneous Determination of Six Constituents of Rutinoid, Quercitrin and Kaempferin

Method used

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
View more

Image

Smart Image Click on the blue labels to locate them in the text.
Viewing Examples
Smart Image
  • Method for determining flavone components in tea leaf by using HPLC
  • Method for determining flavone components in tea leaf by using HPLC
  • Method for determining flavone components in tea leaf by using HPLC

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0037] 1 Standard working fluid configuration

[0038] 1.1 Preparation of standard working solution

[0039] Accurately weigh each standard, add quantitative methanol to dissolve, and prepare the mother solution with the following concentrations: quercitrin (5.0mg / mL), kaempferin (4.2mg / mL), rutin (5.6mg / mL), quercetin Sodium (3.7mg / mL), kaempferol (4.3mg / mL), myricetin (5.2mg / mL).

[0040] 1.2 Drawing of standard curve

[0041] Table 1 Concentration gradient of standard working solution

[0042]

[0043] 1.3 Chromatographic conditions

[0044] Chromatographic column: Waters Sunfire C185μm 4.6×150mm Column; mobile phase: 0.4% acetic acid aqueous solution by volume as phase A, acetonitrile as phase B, gradient elution, gradient elution process see Table 2, Table 3 and Table 4; flow rate : 1mL / min; column temperature: 30°C; UV detector: wavelength 355nm; injection volume: 20μL.

[0045] Table 2 Gradient elution conditions 1

[0046]

[0047] Table 3 Gradient elution co...

Embodiment 2

[0079] Embodiment 2 repeatability, precision experiment

[0080] 2.1 Repeatability experiment

[0081] Select the same sample, use this method to do 5 tests in a short period of time, and get the spectrum as Figure 4 As shown, the relative standard deviation of retention time and peak area is shown in Table 8 below:

[0082] Table 8 Repeatability experiment results

[0083]

[0084] It can be seen from Table 8 that the relative deviation of the retention time of each component is within 0.1, the relative standard deviation of the peak area is less than 2.4, and the method has good repeatability.

[0085] 2.2 Precision experiment

[0086] The same sample was injected 5 times continuously, and the obtained spectrum is as follows Figure 5 As shown, the relative standard deviation of retention time and peak area is shown in Table 9 below:

[0087] Table 9 Results of precision experiments

[0088]

[0089] It can be seen from Table 9 that the relative deviation of the...

Embodiment 3

[0090] Example 3 Applicability of the method to tea samples (select black tea, green tea, and Pu'er tea for comparison)

[0091] Depend on Image 6 , Figure 7 , Figure 8 The comparison shows that in different tea samples, the method can effectively separate the components, the baseline is stable, the peak shape is independent, and the analysis effect is good. The detection results of the flavonoid components of the three teas are shown in Table 10:

[0092] Table 10 Selection of optimal ion extraction solution concentration

[0093]

[0094] It can be seen from Table 10 that the six flavonoids in the three teas can be detected by this method. Based on the similarity of the content of the tea samples, this method is suitable for the six flavonoids in the tea samples. detection.

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

PUM

PropertyMeasurementUnit
Concentrationaaaaaaaaaa
Login to View More

Abstract

The invention discloses a method for determining the flavone components in tea leaf by using HPLC, and applications thereof, and belongs to the field of tea leaf detection. The method specifically comprises: obtaining a sample solution by using a tea leaf sample as a raw material through a chemical extraction method; and carrying out gradient elution, wherein the mobile phase comprises a phase A and a phase B, the phase A uses an acetic acid aqueous solution with a volume ratio of 0.3-1.2%, and the phase B uses acetonitrile. According to the present invention, with the established HPLC method, the six flavone components comprising three flavonoid glycosides and three flavonols in the tea leaf can be simultaneously determined, such that the determination of the total content of the flavone components is improved, and the important significance is provided for the research and the control of the tea leaf quality.

Description

technical field [0001] The invention relates to a detection method for tea, in particular to a method and application for determining flavonoids in tea by HPLC. Background technique [0002] Flavonoids are a class of yellow pigments widely present in nature, also known as anthoxanthins. Its basic structural formula is 2-phenylchromone. Now generally refers to two benzene rings with phenolic hydroxyl groups linked by the middle three carbons 6 -C 3 -C 6 A large class of low molecular weight polyphenolic compounds with carbon frameworks. According to the different modifications of the central C ring of flavonoids, they can be divided into several major categories such as flavonoids, flavonols, flavanols, isoflavones, flavanones and anthocyanins, among which flavonols are the most, accounting for the most in flavonoids. About 1 / 3 of the total. [0003] Flavonoids and their glycosides have a variety of physiological functions, and studies have shown that they have antioxid...

Claims

the structure of the environmentally friendly knitted fabric provided by the present invention; figure 2 Flow chart of the yarn wrapping machine for environmentally friendly knitted fabrics and storage devices; image 3 Is the parameter map of the yarn covering machine
Login to View More

Application Information

Patent Timeline
no application Login to View More
IPC IPC(8): G01N30/02G01N30/06
Inventor 凌登猛陈孝权于星
Owner MENGHAI TEA IND