Selenium-rich isaria feline culture substance and culture method thereof
A culture method and technology of I. clavulae, applied in the field of culture of I. catalis and its cultivation, to achieve the effects of reducing toxicity, increasing supplementation, improving physiological activity and absorption rate
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Embodiment 1
[0049] (1) Medium
[0050] Recipe: 840 parts of millet, 42 parts of glucose, 0.10 part of sodium selenite. Add 100ml of water to sodium selenite to make an aqueous solution.
[0051] Add 2000ml of water and glucose to the casserole, heat to boil, add millet and sodium selenite aqueous solution, stir well, cover the pot, stir with a shovel after 3 minutes, continue heating and boiling for 20 minutes, and keep stirring until the millet becomes solid Turn off the fire when it is in the posture, and let it stand at room temperature. Divide the culture medium into small pieces with a shovel, divide them into bottles, seal them with kraft paper blocks, and wrap them tightly with cotton rope. Put the culture medium in the pressure cooker, a total of 2 layers; place the inoculation needle in a large test tube and seal it, put kraft paper in an envelope and seal it, and wrap them with cotton rope; keep the mouth of the test tube upward, and place it in the gap of the culture medium; ...
Embodiment 2
[0059] (1) Medium
[0060] Recipe: 800 parts of millet, 35 parts of glucose, 0.07 part of sodium selenite. Add 100ml of water to sodium selenite to make an aqueous solution.
[0061] Dissolve glucose in 2000ml water and heat to boiling, then add millet and sodium selenite, continue heating and boiling until the millet becomes solid, stop heating, let it cool to room temperature, and sterilize for later use.
[0062] (2) Inoculation
[0063] The mycelium of I. catalis was cultured in a ventilated environment with a relative humidity of 45% at 22°C as a strain, and was taken out for use after the culture was complete.
[0064] (3) Dried
[0065] Put the cultured mycelium and culture medium together in a drying container, and dry them twice to form mycelium blocks. On the first day, bake at 110°C for 5 hours; on the second day, bake at 80°C for 6 hours.
Embodiment 3
[0067] (1) Medium
[0068] Recipe: 900 parts of millet, 50 parts of glucose, 0.14 parts of sodium selenite. Add 100ml of water to sodium selenite to make an aqueous solution.
[0069] Dissolve glucose in 2000ml water and heat to boiling, then add millet and sodium selenite, continue heating and boiling until the millet becomes solid, stop heating, let it cool to room temperature, and sterilize for later use.
[0070] (2) Inoculation
[0071] The mycelium of I. catalis was cultured in a ventilated environment with a relative humidity of 50% at 18°C as a strain, and was taken out for use after the culture was complete.
[0072] (3) Dried
[0073] Put the fully cultivated mycelium and medium together in a drying container, and dry them twice to form mycelium blocks. On the first day, bake at 95°C for 5 hours; on the second day, bake at 90°C for 6 hours.
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