Lactobacillus paracasei as well as strain domestication method and culture method thereof
A culture method, Lactobacillus technology, applied in biochemical equipment and methods, methods using microorganisms, methods based on microorganisms, etc., can solve the problem of lack of Lactobacillus paracasei products rich in organic selenium, etc., to reduce the toxicity of selenium , Improve physiological activity and absorption rate, enhance the effect of resistance
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Embodiment 1
[0051] (1) Preparation of Lactobacillus paracasei seed medium: 10.0 g of casein, 8.0 g of beef powder, 4.0 g of yeast powder, 20.0 g of glucose, 0.2 g of magnesium sulfate, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, and phosphoric acid Dipotassium hydrogen dipotassium 2.0g, manganese sulfate 0.05g, Tween 80 1.0g and distilled water 1000mL were mixed, the pH value was adjusted to 6.2, and sterilized in an autoclave at a high temperature of 121°C for 20min, and the by volume ratio was 3%. Lactobacillus casei strains were inoculated on Lactobacillus paracasei seed medium for cultivation.
[0052] (2) Adding selenium: Detect the OD value of the fermentation broth every two hours, when it reaches 1.2-1.6, record the total fermentation time h1, and add sodium selenite solution to make the selenium content of the fermentation broth 0.4 μg / g.
[0053] (3) Centrifugation: After adding selenium, ferment for another hour, and then centrifuge the fermentation broth.
[0054] ...
Embodiment 2
[0059] (1) Preparation of Lactobacillus paracasei seed medium: 10.0 g of casein, 8.0 g of beef powder, 4.0 g of yeast powder, 20.0 g of glucose, 0.2 g of magnesium sulfate, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, and phosphoric acid Dipotassium hydrogen dipotassium 2.0g, manganese sulfate 0.05g, Tween 80 1.0g and distilled water 1000mL were mixed, the pH value was adjusted to 6.2, and sterilized in an autoclave at a high temperature of 121°C for 20min, and the by volume ratio was 3%. Lactobacillus casei strains were inoculated on Lactobacillus paracasei seed medium for cultivation.
[0060] (2) Adding selenium: Detect the OD value of the fermentation broth every two hours, when it reaches 1.2-1.6, record the total fermentation time h1, and add sodium selenite solution to make the selenium content of the fermentation broth 0.4 μg / g.
[0061] (3) Centrifugation: After adding selenium, ferment for another hour, and then centrifuge the fermentation broth.
[0062] ...
Embodiment 3
[0067] (1) Preparation of Lactobacillus paracasei seed medium: 10.0 g of casein, 8.0 g of beef powder, 4.0 g of yeast powder, 20.0 g of glucose, 0.2 g of magnesium sulfate, 5.0 g of sodium acetate, 2.0 g of triammonium citrate, and phosphoric acid Dipotassium hydrogen dipotassium 2.0g, manganese sulfate 0.05g, Tween 80 1.0g and distilled water 1000mL were mixed, the pH value was adjusted to 6.2, and sterilized in an autoclave at a high temperature of 121°C for 20min, and the by volume ratio was 3%. Lactobacillus casei strains were inoculated on Lactobacillus paracasei seed medium for cultivation.
[0068] (2) Adding selenium: Detect the OD value of the fermentation broth every two hours, when it reaches 1.2-1.6, record the total fermentation time h1, and add sodium selenite solution to make the selenium content of the fermentation broth 0.4 μg / g.
[0069] (3) Centrifugation: After adding selenium, ferment for another hour, and then centrifuge the fermentation broth.
[0070] ...
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