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Pretreatment method of samples for detection of pyrethroid pesticide residues in wine

A technology for the detection of pyrethroids and pesticide residues, which is applied in the field of pretreatment of samples for the detection of pyrethroids pesticide residues in wine, can solve the problem that the extract has not been concentrated, and achieve high accuracy, fast and accurate measurement, precision good effect

Active Publication Date: 2019-06-07
鹿泽启 +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, the QuEChERS method still needs to add acetonitrile to the extract, the extract is not concentrated, and further purification of the extract is still required

Method used

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  • Pretreatment method of samples for detection of pyrethroid pesticide residues in wine
  • Pretreatment method of samples for detection of pyrethroid pesticide residues in wine
  • Pretreatment method of samples for detection of pyrethroid pesticide residues in wine

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1 The detection test of the recovery rate of the organic dry red wine sample by using the pretreatment method of the detection sample of pyrethroid pesticide residues in wine

[0030] Take 1 bottle of organic dry red wine without pesticide residues, with an alcohol content of 12.5%, shake well, accurately measure 10.00 mL, put it in a 20 mL plastic centrifuge tube, add 2 g of disodium hydrogen phosphate, 3 g of anhydrous magnesium sulfate, N -Propylethylenediamine 150mg, vortexed for 2min, centrifuged (4000r·min -1 ), and then directly take the supernatant for measurement, and the measurement result is a blank sample.

[0031] Same as the above operation, take 3 parts of 10.00mL organic dry red wine, add 10μL of pesticide mixed standard solution respectively, measure the recovery rate, and calculate the measurement results by the standard curve method. The pesticide mixed standard solution is a mixture of 6 kinds of pesticides, including tetramethrin, fenpropat...

Embodiment 2

[0040] Example 2 The detection test of the dry red wine sample by the pretreatment method of the pyrethroid pesticide residue detection sample in wine

[0041] Take 1 bottle of dry red wine with an alcohol content of 12.0%, shake it well, accurately measure 10.00mL, put it in a 20mL plastic centrifuge tube, add 3g of ammonium sulfate, 2g of anhydrous sodium sulfate, and 200mg of N-propylethylenediamine, Vortex for 2 min, centrifuge (4000r·min -1 ), and then directly take the supernatant for measurement, and the measurement result is a blank sample.

[0042] Same as the above operation, take 3 parts of 10.00mL dry red wine, add 10μL of pesticide mixed standard solution respectively, measure the recovery rate, and calculate the measurement results by the standard curve method. The pesticide mixed standard solution is a mixture of 6 kinds of pesticides, including tetramethrin, fenpropathrin, cyhalothrin, cypermethrin, fenvalerate and deltamethrin, and the concentration is 10 μg·mL...

Embodiment 3

[0045] Example 3 The detection test of dry white wine samples by the pretreatment method of pyrethroid pesticide residue detection samples in wine

[0046] Take 1 bottle of dry white wine with an alcohol content of 12.0%, shake it well, accurately measure 10.00 mL, put it in a 20 mL plastic centrifuge tube, add 3 g of dipotassium hydrogen phosphate, 4 g of anhydrous sodium sulfate, N-propyl ethylene di Amine 100mg, vortexed for 2min, centrifuged (4000r·min -1 ), and then directly take the supernatant for measurement, and the measurement result is a blank sample.

[0047] Same as the above operation, take 3 parts of 10.00mL dry white wine, add 10μL of pesticide mixed standard solution respectively, measure the recovery rate, and calculate the measurement results by the standard curve method. The pesticide mixed standard solution is a mixture of 6 kinds of pesticides, including tetramethrin, fenpropathrin, cyhalothrin, cypermethrin, fenvalerate and deltamethrin, and the concent...

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Abstract

The invention discloses a pretreatment method of a pyrethriod pesticide residue detection sample in wine. Ethyl alcohol contained in the wine is used as an extracting agent, a sufficient quantity of a dehydrating agent (anhydrous magnesium sulfate and anhydrous sodium sulfate), split-phase salt (ammonium sulfate and dipotassium phosphate) and a purifying agent (N-primary secondary amine) is directly added into the wine at a time, and supernatant is centrifugally taken directly for chromatography and mass spectrometry analysis. Pesticide residue extraction, concentration and purification are achieved in one-step operation conveniently, and the method is simple and reliable.

Description

technical field [0001] The invention relates to the field of pesticide residue detection, in particular to a pretreatment method for detection samples of pyrethroid pesticide residues in wine. Background technique [0002] Pyrethroids are commonly used broad-spectrum insecticides, and the residues of these pesticides in food and beverages will cause harm to the human body. Studies have shown that pyrethroids can disrupt the normal physiology of animal nerves, thereby causing harm to animals. At present, many countries have strictly controlled the residues of pyrethroids in food and beverages. There have been many reports on the detection of pyrethroids in fruits, vegetables, cereals and other crops and processed agricultural products such as vegetable oil and wine. [0003] The current methods for the quantitative determination of pyrethroid pesticide residues in wine are mainly gas chromatography and gas chromatography-mass spectrometry. These assays are sensitive, preci...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N30/02G01N30/06
CPCG01N30/02G01N30/06
Inventor 鹿泽启
Owner 鹿泽启