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Optimization method for separating target in complex mixture by simulated moving bed chromatography

A technology of simulated moving bed and chromatographic separation, applied in the field of optimization of targets in complex mixtures separated by simulated moving bed chromatography

Active Publication Date: 2017-04-26
UNIV OF SCI & TECH LIAONING
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0005] The purpose of the present invention is to provide a method for optimizing the method for optimizing target substances in complex mixtures by using simulated moving bed chromatography for the separation of target substances in complex mixtures for existing SMB chromatography.

Method used

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  • Optimization method for separating target in complex mixture by simulated moving bed chromatography
  • Optimization method for separating target in complex mixture by simulated moving bed chromatography
  • Optimization method for separating target in complex mixture by simulated moving bed chromatography

Examples

Experimental program
Comparison scheme
Effect test

Embodiment

[0088] An optimized method for the separation of rebaudioside A (RA) from stevia rebaudiana extract by SMB chromatography.

[0089] SMB chromatographic system used: Working mode: 1-1-2, I band is independent, see figure 2 ; 4 chromatographic columns, the chromatographic column specifications are D × L = i0mm × 100mm; stationary phase: C18, 40 ~ 60 μ m; mobile phase: ethanol-water, proportioning 1: 1; chromatographic separation temperature: room temperature;

[0090] HPLC detection conditions: analytical column: 4.6mm * 250mm (C18, 5 μm, Agilent, U.S.A.), mobile phase: the ratio of chromatographic acetonitrile and sodium dihydrogen phosphate buffer solution of pH=2.6 is 32 / 68 (v / v), flow velocity 1.0ml / min, measurement wavelength 213nm, measurement temperature 30°C.

[0091] The optimization method includes the following steps:

[0092] (1) prepare the sample liquid of stevia extract by the mobile phase ethanol of SMB chromatogram and the proportioning of water, measure the ...

Embodiment 2

[0139] Adopt the orthogonal design optimization method to optimize the SMB chromatographic system operating parameters with the SMB chromatographic model, and other steps and conditions are all with embodiment 1; L 9 (3 3 ) orthogonal table and SMB model simulation calculation results are shown in Table 2, and the experimental point with higher production efficiency (g product / ml mobile phase) optimized in Table 2 is, That is, the flow rate of pump D is 0.6 ml / min, the flow rate of pump F is 0.2 ml / min, and the injection concentration is 10 mg / ml.

[0140] Table 2, L 9 (3 3 ) orthogonal table and SMB chromatographic model simulation calculation results

[0141]

[0142] The separation experiment was carried out under the conditions optimized by the SMB chromatographic model, and the results are shown in Table 3.

[0143] Table 3, SMB chromatographic model calculation simulation results and SMB separation experiment results

[0144]

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Abstract

The invention discloses an optimization method for separating a target in a complex mixture by simulated moving bed chromatography. The optimization method comprises the following steps: firstly, determining interfering components of the target in an SMB separation system and imparting concentrations of the interfering components; then, establishing a single-column chromatography model; then establishing an SMB chromatography model in combination with a node model; finally, calculating with the SMB chromatography model, optimizing operation conditions for separating the target from the complex mixture. The optimization method provided by the invention only establishes the single-column chromatography model with an ideal chromatographic model of single-component adsorption isotherm for the target and the interfering components to solve a problem that numerous parameters cannot be measured, adopts difference scheme iteration to respectively solve the single-column chromatography model and the SMB chromatography model to realize advantages of simple dissolving method, good stability and relatively high accuracy, and only needs 2-5 experimental points to establish the SMB chromatography model which can guide the practice of SMB chromatographic separation of complex mixture.

Description

technical field [0001] The invention relates to the technical field of chromatography, in particular to an optimized method for separating target substances in complex mixtures by using simulated moving bed chromatography. Background technique [0002] Simulated moving bed (SMB) chromatographic technology has the characteristics of continuous, automatic and efficient, and has become a high-end separation technology in the fields of petrochemical, fine chemical and pharmaceutical (especially chiral drug resolution and biological product separation). [0003] The SMB system is very complex, and it has many design parameters, including: the total number of chromatographic columns, the number of chromatographic columns configured with each belt, column length, column diameter, bed porosity, dead volume, filler particle size and particle porosity, etc.; it It has many thermodynamic and kinetic parameters, including adsorption isotherm, mass transfer coefficient, diffusion coeffic...

Claims

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Application Information

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IPC IPC(8): G01N30/86
CPCG01N30/8658G01N30/8693
Inventor 王绍艳李守江魏伯峰石爽张伟范嘉园
Owner UNIV OF SCI & TECH LIAONING
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