Cell preservation solution as well as preparation method and use method thereof

A technology of preservation solution and cells, applied in the field of cell preservation solution, can solve problems such as efficient cell preservation, and achieve the effect of blocking cell apoptosis, good effect and enhancing protective effect.

Inactive Publication Date: 2017-05-10
MIRACLEAN TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Aiming at the problem that 4% paraformaldehyde cell preservation solution cannot efficiently preserve cells for a long time, the present invention provides a new cell preservation so

Method used

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  • Cell preservation solution as well as preparation method and use method thereof
  • Cell preservation solution as well as preparation method and use method thereof
  • Cell preservation solution as well as preparation method and use method thereof

Examples

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Embodiment Construction

[0030] The content of the present invention is further described below by way of examples.

[0031] 1. Preparation of paraformaldehyde preservation solution

[0032] Dissolve the weighed paraformaldehyde in 5wt% sodium hydroxide aqueous solution at 38°C, then use ultrapure water to make up 8wt% paraformaldehyde solution, and then stir and drop 0.23% v / v coke dicarbonate Ethyl ester, stirring continuously until the solution is clear, and then adding PB buffer solution until the mass percentage of paraformaldehyde is 4wt%. When dissolving, the amount of sodium hydroxide solution used should not be too much, and the paraformaldehyde can be submerged to make it dissolve quickly. Utilize the preparation method of the preservation solution of paraformaldehyde in this embodiment, 2 hours faster than the method directly dissolving with PB buffer solution.

[0033] In this embodiment, the PB buffer used is 0.2mol / L NaH 2 PO 4 and 0.2mol / L Na 2 HPO 4 It is prepared by mixing at a ...

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Abstract

The invention discloses a cell preservation solution. The cell preservation solution is a solution prepared from ultra pure water. The solution is a paraform preservation solution. The solution also comprises 0.4-0.8wt% of glutaraldehyde, 0.05-0.1mol/L potassium citrate, 0.035-0.15mol/L sodium diacetate, 0.1-0.3mol/L magnesium chloride hexahydrate, 0.08-0.4mol/L sodium chloride, 0.003-0.005mol/L alpha-lipoic acid, 0.08-0.12mol/L trehalose and 3-5mg/L matrine. The pH value of the cell preservation solution is 7.1-7.4, and the osmotic pressure of the cell preservation solution is 290-315mmol/L. According to the preservation solution disclosed by the invention, the cell preservation time is greatly prolonged, besides, the preparation method is simple, and the application range is wide.

Description

technical field [0001] The invention belongs to the technical field of biological preparations, and in particular relates to a cell preservation solution, a preparation method and a use method of the preservation solution. Background technique [0002] Gene extraction is the most basic application technology in molecular biology testing. Commonly used clinical methods include sodium iodide method, phenol-chloroform method, Chelex-100 method and kit method. Most of the above-mentioned methods use human whole blood as the sample for gene extraction, and there are certain difficulties in the sampling process, such as safety hazards in the blood collection process, blood samples are easily contaminated, and the patient’s rejection rate is high. It is cumbersome and requires personnel with professional skills to operate reliably. Therefore, it is of great significance to explore gene collection methods that are easier to obtain materials, more convenient to operate, and easier t...

Claims

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Application Information

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IPC IPC(8): A01N1/02
CPCA01N1/0205A01N1/0226
Inventor 董占龙
Owner MIRACLEAN TECH
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