Application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii

A technology for Phytophthora downy mildew and Botrytis cinerea of ​​litchi is applied in the biological field and can solve the problems of drug resistance of pathogenic bacteria, restriction of post-harvest storage and transportation of fruits, browning of peels, etc. Human health and the effect of reducing toxic and side effects

Active Publication Date: 2017-05-10
CHINA AGRI UNIV +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Downy blight caused by Peronophythora litchi Chen ex Ko et al. is the most important disease in postharvest storage and preservation of litchi. Under the condition of suitable temperature and humidity, the pathogen rapidly infects and expands, causing peel Browning, pulp rot and exudation of wine sour juice severely limit the post-harvest storage and transportation of the fruit
[0004] At present, the control of postharvest diseases of fruits is mainly based on chemical agents, but there is a risk of pesticide residues endangering human health and causing pathogenic bacteria to develop drug resistance due to improper use.

Method used

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  • Application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii
  • Application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii
  • Application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0040] Embodiment 1, pterostilbene to the bacteriostatic activity of botrytis cinerea and litchi downy mildew

[0041] Detection by mycelium growth rate method:

[0042] Pathogen tested: Botrytis cinerea isolated from diseased grape fruit , Litchi downy mildew Peronophythora litchii (Cai Xueqing, Lin Na, Chen Wei, et al. Biological characteristics of litchi downy mildew [J]. Tropical Crops, 2009,30(9):1226-1231) isolated from diseased litchi fruit.

[0043] Main reagents: Pterostilbene was purchased from Bailingwei Technology Co., Ltd., with a purity of >98%.

[0044] Potato dextrose agar medium PDA was used to cultivate botrytis cinerea, and carrot juice agar medium CA was used to cultivate litchi downy mildew.

[0045] The inhibitory effect of different concentrations of pterostilbene on the growth of pathogenic fungi was determined by the method of mycelial growth rate. The specific operation: (1) use phosphate buffered saline (PBS; 50.0mmol / L, pH7.4, formula: if prepari...

Embodiment 2

[0047] Embodiment 2, pterostilbene to the bacteriostatic activity of botrytis cinerea and litchi downy mildew

[0048] Spore germination method:

[0049] Preparation of pathogenic spore / sorangia suspension: Cultivate botrytis cinerea and litchi downy mildew on PDA or CA medium for 5 days, then add 5 mL of sterile water to the plate for washing and shaking to prepare spores / spores capsule suspension mother liquor. Add potato dextrose liquid medium (PDB) to the botrytis cinerea spore suspension mother liquor to obtain the diluted botrytis cinerea spore suspension; add sterile water to the lychee downy mildew sporangia suspension to obtain the diluted litchi Sporangia suspension of Phytophthora downy mildew; dilute the spore / sorangia suspension of the pathogen to a concentration of 10 5 individual / mL.

[0050] The specific operation of the spore germination method: (1) use phosphate buffered saline (PBS; 50.0mmol / L, PH7.4):NaOH aqueous solution (1.0mol / L, PH13) (volume ratio i...

Embodiment 3

[0055] Example 3, Pterostilbene control effect experiment on postharvest grape gray mold (B.cinerea)

[0056] Test materials: the test grape variety "mare milk", purchased from the market. Artificially separate the single-grain grape fruit, leaving the fruit stalk, select healthy fruit particles with the same size, shape and maturity, and sterilize them in 2% sodium hypochlorite aqueous solution for 2 minutes, then rinse them twice with sterile water, and dry them in the air Post-group processing.

[0057] Specific operation: (1) use a sterile inoculation needle to pierce a wound with a depth of 2 mm and a diameter of 1.5 mm at the equator of the fruit; (2) insert 10 μL of 100, 250, 500, 1000, 10000 μg / mL pterostilbene solution (phosphate buffered saline (PBS; 50.0mmol / L, PH7.4): NaOH aqueous solution (1.0mol / L, PH13) (volume ratio 9:1) as solvent), each Three repetitions were set up for each concentration treatment, and a total of 60 fruits were inoculated, and the same vol...

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Abstract

The invention discloses application of pterostilbene to prevention and treatment of botrytis cinerea and peronophythora litchii, provides application of pterostilbene or a solution thereof to inhibition of peronophythora litchii and also provides application of pterostilbene or a solution thereof to prevention and treatment of peronophythora litchii. Experiments show that pterostilbene can be used for preventing and treating botrytis cinerea and peronophythora litchii of harvested grapes and litchis; and pterostilbene can be separately used, can also be combined with other antibacterial agents for improving the antibacterial effect or reducing the toxic and side effects, is green and safe, is beneficial to human health, meets the food safety requirements and achieves the effects of preventing and treating botrytis cinerea and peronophythora litchi and retaining freshness of fruits.

Description

technical field [0001] The invention relates to the field of biotechnology, in particular to the application of pterostilbene in preventing and treating botrytis cinerea and litchi downy mildew. Background technique [0002] Both grapes and lychees are important economic crops and have become the pillars of many rural economic developments. However, the two fruits are not easy to store and keep fresh. Inventing new preservatives or developing new fresh-keeping techniques to control their post-harvest diseases is important in the post-harvest of the two fruits. Adding value and extending the supply period will play a crucial role. [0003] Botrytis cinerea is one of the most important diseases of grapes after harvest. The pathogen is Botrytis cinerea, which can lead to fruit rot and threshing of grapes during sales and low-temperature storage. Downy blight caused by Peronophythora litchi Chen ex Ko et al. is the most important disease in postharvest storage and preservation ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A23B7/154
CPCA23B7/154
Inventor 高灵旺徐丹丹习平根姜子德李敏慧江立群韩亭宇香梅
Owner CHINA AGRI UNIV
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