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Rhizoma Belamcandae decoction pieces rich in flavonoid aglycones and preparation technology of Rhizoma Belamcandae extract

A technology for extracting and flavonoid aglycone of Shegan, which is applied in the field of preparing high-efficiency decoction pieces of Shegan rich in flavonoid aglycone and its extract, can solve the problems of not separating flavonoid aglycone, and achieve the effects of simple method and low impurity content.

Active Publication Date: 2017-05-10
辽宁省中医药研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the extraction of total flavonoids from Shegan, there are also reports of adsorption resin separation, but so far, the reported methods are all for the preparation of total flavonoid extracts, and there is no research aimed at the separation of flavonoid aglycones

Method used

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  • Rhizoma Belamcandae decoction pieces rich in flavonoid aglycones and preparation technology of Rhizoma Belamcandae extract
  • Rhizoma Belamcandae decoction pieces rich in flavonoid aglycones and preparation technology of Rhizoma Belamcandae extract
  • Rhizoma Belamcandae decoction pieces rich in flavonoid aglycones and preparation technology of Rhizoma Belamcandae extract

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0023] Optimization of enzymatic conversion process of total aglycon in Shegan

[0024] Instruments: Agilent 1100 liquid chromatography system (Agilent Corporation, USA), Phenomenex chromatographic column (Phenomenex Synergi 4u polar-RP 80A 250×4.6mm 4micron), PHS-25CPH meter (Shanghai Tangyi Instrument Co., Ltd.), KQ-250DB numerical control ultrasonic Cleaner (Kunshan Ultrasonic Instrument Co., Ltd.), DZF-6050 vacuum drying oven (Gongyi Yuhua Instrument Co., Ltd.).

[0025] Test drugs: Shegan Decoction Pieces (Anhui Huqiao Traditional Chinese Medicine Decoction Pieces Factory, batch number: 20141120); citric acid (Sinopharm Chemical Reagent Co., Ltd., batch number: 20140415), sodium citrate (Shenyang Reagent No. 1 Factory, batch number: 880703); β-glucose Glucosidase (Hubei Dahua Weiye Pharmaceutical Chemical Co., Ltd., batch number: 20150103 potency: 100,000 U / mg).

[0026] Taking the content of total glycosides and total aglycones in Shegan as the investigation index, the ...

Embodiment 2

[0030] The technical method of enriching and purifying total aglycones with commercialized D101 macroporous adsorption resin, the steps are as follows:

[0031] (1) At 45°C, add 40 million U of β-glucosidase to 1Kg Shegan decoction pieces, soak in citric acid-sodium citrate buffer solution with pH=4.5 for 48 hours, take it out, and drain the water , dried, crushed, and then refluxed with 8 times and 6 times the volume of 70% ethanol to extract twice, each time for 2 hours, the combined filtrate was distilled under reduced pressure to recover ethanol, and the alcohol concentration was adjusted to 30%, standing for 12 hours, centrifuged to remove a small amount of insoluble material to obtain 2000ml of adsorption solution containing 0.5g of crude drug per 1ml.

[0032] (2) Pass 2BV of the above-mentioned adsorption solution through a resin column equipped with D101 macroporous adsorption resin (column inner diameter 10cm, column length 150cm, 6000mL wet resin is housed), and the...

Embodiment 3

[0038]The difference from Example 2 is that in step (1), the amount of β-glucosidase added is 50 million U, soaked in citric acid-disodium hydrogen phosphate buffer solution with pH=5 for 24 hours. Finally, 31.60 g of total aglycone extract was obtained, and determined by HPLC analysis, the total aglycon content in the extract was 15.5% (w%).

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Abstract

The invention aims to provide Rhizoma Belamcandae decoction pieces rich in flavonoid aglycones and a preparation technology of Rhizoma Belamcandae extract. The preparation technology specifically includes the steps of adding 20 million-60 million U / kg beta-glucosidase into Rhizoma Belamcandae at 25-55 DEG C, soaking in a buffer solution at a pH value of 4-5 for 24-48 hours, taking the soaked Rhizoma Belamcandae out, draining off the moisture, drying, and sieving to remove chippings to obtain the Rhizoma Belamcandae decoction pieces rich in flavonoid aglycones, wherein the Rhizoma Belamcandae refers to a Rhizoma Belamcandae crude medicinal material, decoction pieces obtained from the cut crude medicinal materials or crushed medicinal material powder. The preparation technology has the advantages that the preparation technology is capable of remarkably increasing the content of the flavonoid aglycones (the content of tectorigenin and irigenin of the flavonoid aglycones is more than twice as much as that of tectorigenin and irigenin of flavonoid aglycones of the Rhizoma Belamcandae crude medicinal materials), so that antibacterial anti-inflammatory effects of the decoction pieces are improved; a macroporous adsorbent resin technology can be used for further enriching and purifying total aglycones, so that further preparation development is guaranteed.

Description

technical field [0001] The invention belongs to the technical field of Chinese herbal medicines, and particularly provides a process for preparing flavonoid aglycon-rich Shegan high-efficiency decoction pieces and extracts thereof through an enzymatic reaction. Background technique [0002] Shegan is a processed product of the dried rhizome of Belamcanda chinensis (L.) DC., a plant of the family Iridaceae. It is mostly used for stagnation of heat, toxin, phlegm and fire, sore throat, excessive phlegm and salivation, cough and asthma. As an important medicine for treating upper respiratory diseases, Shegan contains flavonoids and their derivatives, ketones, phenols, benzenes, bicyclic triterpenes, steroids and volatile components. Among them, flavonoids are the main pharmacological active ingredients, which have anti-inflammatory, anti-oxidation, antibacterial and antiviral effects. [0003] There are more than 30 kinds of flavonoids (including glycosides and aglycones) rep...

Claims

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Application Information

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IPC IPC(8): A61K36/88
CPCA61K36/88A61K2236/19A61K2236/331A61K2236/333A61K2236/55
Inventor 李国信
Owner 辽宁省中医药研究院
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