A dual-mode molecular imaging probe integrating optics and photoacoustics and its preparation method and application

A molecular imaging, dual-modality technology, applied in preparations for in vivo experiments, echo/ultrasound imaging agents, general/multifunctional contrast agents, etc., to achieve good optical imaging quality and achieve optical and photoacoustic imaging effects.

Active Publication Date: 2020-02-14
THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0008] Through the above analysis, the previous inventions all related to the use of nucleic acid nanostructures as drug carriers and small animal photothermal therapy, but there is no report on the use of nucleic acid nanomaterials as carriers to combine gold nanorods and quantum dots.

Method used

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  • A dual-mode molecular imaging probe integrating optics and photoacoustics and its preparation method and application
  • A dual-mode molecular imaging probe integrating optics and photoacoustics and its preparation method and application
  • A dual-mode molecular imaging probe integrating optics and photoacoustics and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0057] Example 1 Preparation of nucleic acid nanostructure-gold nanorod-quantum dot complex

[0058] (1) Synthesis of nucleic acid nanostructures

[0059] Mix the M13 phage genomic DNA at a final concentration of 10 nM, the staple strand and the captured staple strand at a final concentration of 100 nM at a final concentration of 1× TAE / Mg 2+ (pH=8.3) solution, the final volume of the mixed solution was 100 μL. Control the temperature of the above mixed solution from 95° C. to 20° C. and gradually anneal, and control the whole cooling process to more than 12 hours, so as to obtain a predetermined nucleic acid nanostructure.

[0060](2) Using the seed-induced growth method, the surfactant is cetyltrimethylammonium bromide (CTAB), and the reducing agent is ascorbic acid. By controlling AgNO 3 concentration to prepare gold nanorods (see Chem Mater, 2003, 15(10): 1957-62.). The specific method is as follows:

[0061] (a) Synthesis of seed crystals: take 7.5 mL of cetyltrimethy...

Embodiment 2

[0076] Example 2 In vivo optical imaging of nucleic acid nanostructure-gold nanorods-quantum dots

[0077] Take 100 μL each of the nucleic acid nanostructure-gold nanorod-quantum dot complex prepared in Example 1 and the nucleic acid nanostructure, gold nanorod and quantum dot mixture of the control group in female BALB / C nude mice at 5-6 weeks The drug was administered subcutaneously on the back, and after the injection, live optical imaging was performed on nude mice using a small animal optical imaging system.

[0078] Such as figure 2 Shown are optical imaging results of nude mice after sample injection. Depend on figure 2 It can be seen that the optical signal of the quantum dots assembled on the nucleic acid nanostructure loaded with gold nanorods is significant compared to the optical signal of the case where the nucleic acid nanostructures, gold nanorods and quantum dots are mixed without assembly, which shows that the After assembly, the nucleic acid nanostructur...

Embodiment 3

[0079] Example 3 Biomimetic Photoacoustic Imaging of Nucleic Acid Nanostructure-Gold Nanorod-Quantum Dot Composite

[0080] Figure 3 shows the photoacoustic imaging results of nucleic acid nanostructure-gold nanorod-quantum dot composite, nucleic acid nanostructure, gold nanorod and quantum dot mixture, and free gold nanorod samples, and compared experiments in three groups. The gold nanorod group (100 μL), the nucleic acid nanostructure-gold nanorod-quantum dot (100 μL) complex and the nucleic acid nanostructure, gold nanorod and quantum dot mixture (100 μL) were added to the phantom body for photoacoustic experiments. The photoacoustic signals were collected at concentrations of 6nM, 3nM, 1.5nM, 0.75nM, and 0.375nM, respectively. in Figure 3-A It is the phantom photoacoustic imaging result of the nucleic acid nanostructure-gold nanorod-quantum dot composite and the nucleic acid nanostructure, gold nanorod and quantum dot mixture sample, and the left circle of each imaging ...

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Abstract

The invention relates to an optical and opto-acoustic integration dual-mode molecular image probe as well as a preparation method and application thereof. The optical and opto-acoustic integration dual-mode molecular image probe is formed by a nucleic acid nanostructure-gold nanorod-quantum dot compound; the nucleic acid nanostructure is of a two-dimensional and / or three-dimensional nano structure constructed on the basis of a DNA origami technology; the gold nanorod is subjected to surface DNA modification. The nucleic acid nanostructure-gold nanorod-quantum dot compound serving as the optical and opto-acoustic image probe can ensure that the coupled opto-acoustic absorption and comparison effect is enhanced; furthermore, loaded quantum dots are extremely high in optical imaging quality, so that the detection sensitivity can be obviously improved, and the living imaging detection time is prolonged. The material preparation method is simple in process, low in cost, convenient and feasible.

Description

technical field [0001] The present invention relates to the field of optical photoacoustic imaging, specifically, the present invention relates to a self-assembled bimodal nanomolecular imaging probe based on nucleic acid nanostructures, which integrates optics and photoacoustics, and its preparation method and application . Background technique [0002] Malignant tumor is an important disease that seriously endangers human health and is the number one killer threatening human life. In the face of the increasing incidence and mortality of malignant tumors worldwide, as well as the limitations of current diagnosis and treatment methods, the development of an integrated treatment method with high efficiency and low toxicity has become an urgent and major issue. How to effectively use less invasive and highly sensitive molecular imaging methods for early screening and intraoperative navigation of malignant tumors, and how to further realize the integration of tumor diagnosis a...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A61K49/00A61K49/22
CPCA61K49/0002A61K49/0052A61K49/0067A61K49/0093A61K49/222
Inventor 丁宝全王婷蒋乔宋琳琳湛鹏飞李彤彤
Owner THE NAT CENT FOR NANOSCI & TECH NCNST OF CHINA
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