Method for improving yield of hyaluronic acid with high molecular weight

Abstract

The invention relates to a method for improving yield of hyaluronic acid with a high molecular weight. The method comprises the following steps: (1) performing primary treatment on fermentation liquor; (2) purifying the fermentation liquor; and (3) drying hyaluronic acid. The purification method provided by the invention is excellent in purification effect, particularly high in protein removal rate, the yield of hyaluronic acid products is high and can reach 8-10g / L, the recovery rate can be 95%, the service performance is excellent, the solubility is high, and the light transmittance reaches 99%.

Description

technical field [0001] The invention belongs to the field of microbial fermentation, in particular to a method for producing high-molecular-weight hyaluronic acid. Background technique [0002] At present, most domestic enterprises that produce hyaluronic acid by microbial fermentation adopt a long fermentation cycle, generally around 36-40, the product has low molecular weight, poor light transmittance (generally around 90%), and high protein content. The content of glucuronic acid is generally 35-38%. The form of hyaluronic acid produced by some manufacturers is fibrous. This kind of hyaluronic acid is difficult to dissolve, generally takes about 2 days, and the yield is less than 75%. Demanding market, the production process of hyaluronic acid needs to be improved urgently. [0003] At present, most of them use fermentation method to produce HA. As early as 1983, Shiseido in Japan used Streptococcus fermentation to produce hyaluronic acid. Subsequent studies on the prod...

AI Technical Summary

Problems solved by technology

Since the fermentation broth contains substances such as bacteria, protein, and nucleic acid, the methods for extracting HA from the fermentation broth mainly include the following: A. A large number of dilution membrane filtration methods, because there are many impurities such as bacteria in the fermentation broth, it is easy to cause Membrane pores are clogged, and a large amount of dilution is easy to cause excessive operating volume; B. High-speed centrifugation method requires high equipment requirements when removing bacteria; C. Chloroform method, more HA is entrained in the bacteria precipitation, and the recovery rate of HA low, and chloroform pollutes the environment; D. ethanol precipitation method is a relatively commonly used method, but because the equipment and workshops used in the operation of this method must be explosion-proof, this will inevitably increase the risk of operation and production costs

[0004] In the existing methods, there is generally no way to remove the trace content of hyaluronic acid protein, and the trace protein content is likely to cause allergic (different) reactions, especially in the field of cosmetics, the trace protein content still exists greater harm