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Composite strain fermentation agent and preparation method of silage

A technology of compound bacteria and silage, applied in the field of feed biology, can solve the problems of lack of inhibition of miscellaneous bacteria, loss of nutrients, insufficient fermentation, etc.

Inactive Publication Date: 2017-05-10
中粮生物科技(北京)有限公司
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  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

The silage inoculant does not have enough fermentation strength in a short period of time, and it takes a long time for the silage speed to reach a pH value of 4, and the inhibition of miscellaneous bacteria is insufficient, which easily causes the loss of nutrients

Method used

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  • Composite strain fermentation agent and preparation method of silage
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  • Composite strain fermentation agent and preparation method of silage

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preparation example Construction

[0022] In the present invention, the preparation method of described composite strain starter preferably comprises the following steps:

[0023] respectively inoculating Lactobacillus plantarum strains, Enterococcus faecium strains and Lactobacillus Brucella strains into the MRS broth medium and culturing them to obtain Lactobacillus plantarum strains, Enterococcus faecium strains and Lactobacillus Brucella strains;

[0024] The bacterial liquid of Lactobacillus plantarum, the liquid of Enterococcus faecium and the liquid of Lactobacillus brinneri are mixed to prepare a composite strain starter.

[0025] In the present invention, the mixing volume ratio of the Lactobacillus plantarum liquid, the Enterococcus faecium liquid and the Lactobacillus Brucella liquid is preferably 1-2:1-2:1.

[0026] In the present invention, the composition of the MRS broth medium (g / L): peptone 10.0, beef powder 10.0, yeast powder 5.0, glucose 20.0, magnesium sulfate 0.1, sodium acetate 5.0, ammoni...

Embodiment 1

[0046] 1.1 Strains

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Abstract

The invention provides a composite strain fermentation agent and a preparation method of silage. The composite strain fermentation agent comprises the following components: lactobacillus plantarum, enterococcus faecium and lactobacillus buchneri. By adding the composite strain fermentation agent when whole-crop corns are silaged, nutritional ingredients in silaged corns can be well kept, a pH value of the silaged whole-crop corns is rapidly reduced, content of ammoniacal nitrogen is reduced, lactic acid content is improved, a lactic acid fungus quantity is increased, and quality of the silaged whole-crop corns is improved. An experimental result shows that the silaged whole-crop corns obtained by adding the composite strain fermentation agent provided by the invention has the pH value of smaller than or equal to 4.0, mass content of lactic acid of greater than or equal to 7.5% and mass content of ammoniacal nitrogen of smaller than or equal to 2.5%.

Description

technical field [0001] The invention relates to the field of feed biotechnology, in particular to a preparation method of a composite strain starter and silage. Background technique [0002] Silage is a succulent feed prepared and preserved by the fermentation of microorganisms such as lactic acid bacteria under anaerobic conditions after proper processing of fresh green feed. It can not only maintain the green and juicy characteristics of raw materials, but also It has a special sour aroma, soft texture, rich nutrition and good palatability. In recent years, with the rapid development of the animal husbandry economy, the use of silage to provide a balanced supply of green and juicy feed throughout the year has become an important means for the healthy development of the dairy industry. In order to obtain ideal fermentation quality, the number of lactic acid bacteria in the silage raw material should reach 10 5 cfu / g or above. However, in general, the number of lactic aci...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K10/12A23K10/30C12R1/25C12R1/225C12R1/46
CPCC12N1/20A23V2400/11A23V2400/169
Inventor 雷恒
Owner 中粮生物科技(北京)有限公司
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