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Chitosan oligosaccharide complex and preparation method thereof

A technology of chitosan oligosaccharides and complexes, which is applied in the field of chitosan oligosaccharides complexes and their preparation, can solve the problems that the application of preparation methods has not been reported, and achieve shortening of the incubation period and logarithmic growth phase, lower blood sugar, good effect effect

Inactive Publication Date: 2017-05-17
青岛麦迪尔生物工程有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There have been some studies at home and abroad on the simple application of chitosan oligosaccharides to lower blood sugar, but the preparation method of manganese chelated with chitosan oligosaccharides with specific molecular weight and its application in lowering blood sugar have not been reported yet.

Method used

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  • Chitosan oligosaccharide complex and preparation method thereof
  • Chitosan oligosaccharide complex and preparation method thereof

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Effect test

Embodiment 1

[0036] In this embodiment, a chitosan oligosaccharide complex, wherein the deacetylation degree of the chitosan oligosaccharide is ≧95%, the molecular weight range is 1000-3000Da, and the content of manganese is 5%-8%. Oligochitosan and Mn +4 Coordination forms chitooligosaccharides to chelate manganese. The preferred preparation process is as follows:

[0037] 1. Preparation of specific molecular weight chitosan oligosaccharides:

[0038] ① Weigh 100 grams of chitosan (deacetylation degree ≧ 95%) sample and dissolve it in 1 L of glacial acetic acid solution with a mass percentage of 2%.

[0039] ② Add 2 grams of chitosanase (enzyme activity unit 180U / g), and stir at 45°C for 3 hours.

[0040] ③ Add 42 grams of sodium hydroxide, let stand at room temperature for 30 minutes, centrifuge at 8000 rpm / min for 10 minutes, and discard the precipitate.

[0041] ④ After centrifugation, the supernatant was ultrafiltered with a hollow cellulose membrane with a molecular weight cut-of...

Embodiment 2

[0049] Experiment on the effect of chitosan oligosaccharide chelated manganese on pancreatic beta cell line NIT-1:

[0050] The islet β cell line NIT-1 was cultured in DMEM medium containing 100 mL / L fetal bovine serum, and after growing to a monolayer in the culture flask, it was digested with 2.5 g / L trypsin to make a single-cell suspension to The concentration of 5.0×107 / L was inoculated on a 96-well plate. In the experimental group, different concentrations of chitosan oligosaccharide chelated manganese were added, and cultured in a constant temperature incubator with 50 mL / L CO2, 37°C and saturated humidity. The culture solution of the sample was used as the control group, which was taken out at 24, 48, 72, 120, 144 and 168 hours after adding the sample, and the absorbance value (A value) was measured at 492 nm on a microplate reader by MTT method.

[0051] figure 1 The effects of different concentrations of chitosan oligosaccharide chelated manganese on the proliferatio...

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Abstract

The invention provides a chitosan oligosaccharide complex which is chitosan oligosaccharide chelated manganese prepared from chitosan oligosaccharide of which the molecular weight is 1000-3000 Da, and Mn<+4> in coordination. Mn<+4> is chelated with the 1000-3000 Da chitosan oligosaccharide, and tests show that the chitosan oligosaccharide chelated manganese is capable of remarkably promoting growth of pancreatic beta cells, the incubation period and the logarithmic phase time can be shortened, growth of islet cells can be remarkably promoted, and the chitosan oligosaccharide complex has a good effect on blood glucose reduction.

Description

technical field [0001] The invention belongs to the field of biotechnology, and in particular relates to a chitosan oligosaccharide complex and a preparation method thereof. Background technique [0002] Diabetes mellitus (DM) is a common endocrine and metabolic disease with a genetic predisposition. It is a systemic systemic disorder characterized by elevated blood sugar and urine sugar caused by absolute or relative insulin deficiency and leading to disorders of sugar, protein, and fat metabolism. Chronic metabolic diseases are often accompanied by various complications such as cardiovascular and cerebrovascular, kidney, eye and nerve, and are very harmful. According to the WHO report, diabetes has become the third chronic non-communicable disease that seriously threatens human health after malignant tumors and cardiovascular and cerebrovascular diseases in the world. [0003] The valences of manganese are +2, +3, +4, +6 and +7. where +2 (Mn 2+ compounds), +4 (manganese...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08A61P3/10
CPCC08B37/003
Inventor 刘晓艳公衍军
Owner 青岛麦迪尔生物工程有限公司