Method for extracting chondroitin sulfate in pig nasal bones with complex enzymes

A technology of chondroitin sulfate and pig nose bone, which is applied in the biological field, can solve the problems of high cost, low yield and purity, and achieve the effects of increasing yield and purity, reducing production cost, and increasing the purification process

Inactive Publication Date: 2017-05-24
QINGDAO JIULONG BIO PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, the methods mainly used by chondroitin sulfate manufacturers are high in cost, low in yield and purity

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0021] 1. Complex enzymatic hydrolysis

[0022] Take by weighing 100 kilograms of dry pork nose bone and pulverize to powder, add its 400L water. Heat to 80°C, keep it warm for 5.5 hours, and then cool down. When the temperature was lowered to 59°C, 6% compound enzyme was added (the mass ratio of protease, amylase, and pectinase was 47:31:22), and the enzymatic hydrolysis was carried out with stirring for 14 hours.

[0023] 2. Salt solution

[0024] Use hydrochloric acid to adjust the pH of the compound enzyme hydrolyzate to 7.3, add 2% sodium chloride solution according to 13% of the volume of the compound enzyme hydrolyzate, stir and dissolve, heat up to 85°C, keep warm for 90min, then cool down to room temperature, Filter with double gauze; leave the filtrate and measure the quality for later use.

[0025] 3. Secondary enzymatic hydrolysis

[0026] Use 1% sodium hydroxide solution to adjust the pH of the filtrate to 8.9, add trypsin according to the mass ratio of filtra...

Embodiment 2

[0034] 1. Complex enzymatic hydrolysis

[0035] Take by weighing 100 kilograms of dry pork nose bone and pulverize to powder, add its 400L water. Heat to 80°C, keep the temperature for 5 hours, and then cool down. When the temperature was lowered to 53°C, 2% compound enzyme was added (the mass ratio of protease, amylase, and pectinase was 47:31:22), and the enzymolysis was carried out for 13 hours while incubating and stirring.

[0036] 2. Salt solution

[0037] Use hydrochloric acid to adjust the pH of the compound enzyme hydrolyzate to 6.3, add 2% sodium chloride solution according to 14% of the volume of the compound enzyme hydrolyzate, stir and dissolve, heat up to 85°C, keep it warm for 2 hours, and then cool down to room temperature , and filter with double gauze; leave the filtrate and measure the quality for later use.

[0038] 3. Secondary enzymatic hydrolysis

[0039] Use 1% sodium hydroxide solution to adjust the pH of the filtrate to 8.1, add trypsin according ...

Embodiment 3

[0047] 1. Complex enzymatic hydrolysis

[0048] Take by weighing 100 kilograms of dry pork nose bone and pulverize to powder, add its 400L water. Heat to 80°C, keep the temperature for 5 hours, and then cool down. When the temperature was lowered to 56°C, 4% compound enzyme was added (the mass ratio of protease, amylase, and pectinase was 47:31:22), and the enzymatic hydrolysis was carried out with stirring for 12 hours.

[0049] 2. Salt solution

[0050] Use hydrochloric acid to adjust the pH of the compound enzyme hydrolyzate to 6.8, add 2% sodium chloride solution according to 12% of the volume of the compound enzyme hydrolyzate, stir and dissolve, heat up to 85°C, keep it warm for 2 hours, and then cool down to room temperature , filter with double gauze; leave the filtrate and measure the quality for later use.

[0051] 3. Secondary enzymatic hydrolysis

[0052]Use 1% sodium hydroxide solution to adjust the pH of the filtrate to 8.4, add trypsin according to the mass ...

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PUM

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Abstract

The invention provides a method for extracting chondroitin sulfate in pig nasal bones with complex enzymes. Firstly, the complex enzymes are used for extracting chondroitin sulfate in the pig nasal bones, an ultrafiltration membrane is used for further purifying chondroitin sulfate, and chondroitin sulfate is extracted from the pig nasal bones through the steps of complex enzyme enzymolysis, salt decomposition, secondary enzymolysis, ultrafiltration membrane purification and dehydration. In conclusion, compared with a traditional extraction method, no ethyl alcohol is needed, and the purity and yield are obviously improved.

Description

technical field [0001] The invention belongs to the field of biotechnology, and belongs to a method for extracting chondroitin sulfate from pig nasal bones. Background technique [0002] Chondroitin sulfate is an acidic mucopolysaccharide widely present in cartilage, laryngeal bone, nasal bone and trachea of ​​mammals. Chondroitin sulfate is a disaccharide polymer formed by alternating links of glucuronic acid and hexosamine. It contains 50-70 disaccharide basic structures and a molecular weight of 10,000-50,000. According to its chemical composition and structure, it can be divided into CSA, C, D, E, F, H and other isomers. The medicinal chondroitin sulfate is extracted from animal cartilage, mainly A, C and various isomers. A mixture of chondroitin sulfates. Both CSA and CSC are composed of D2 glucosamine aldehyde and N2 acetyl 2D2 galactosamine, but the position of the sulfate group is different. [0003] Chondroitin sulfate usually exists in the form of glycoprotein c...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C08B37/08
CPCC08B37/0069
Inventor 刘乃山姜德志迟培升
Owner QINGDAO JIULONG BIO PHARMA
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