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Soft and transparent silk fibroin membrane and preparation method thereof

A technology of silk fibroin and transparent silk, which is applied in the field of transparent film materials and their preparation, can solve the problems of difficulty in being transparent and soft, the cytotoxicity of small molecular substances is high, and it is difficult to fully meet the requirements for the use of biological materials, so as to maintain biocompatibility. Effect

Active Publication Date: 2017-05-24
NANTONG TEXTILE & SILK IND TECH RES INST
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The Chinese invention patent with publication number CN 103861149 A prepared an insoluble silk fibroin film by blending silk fibroin with small molecular formamide, acetamide, etc., pouring and drying to form a film, but the small molecular substance used High cytotoxicity, it is difficult to fully meet the requirements for the use of biological materials
The Chinese invention patent with the publication number CN 101967282B prepared a transparent silk film by blending small molecular polyols with silk fibroin and pouring and drying to form a film. However, the aggregated structure of the formed silk film is mainly semi-stable. Silk Ⅰ type crystal, it is difficult to maintain the characteristics of transparency and softness for a long time under wet conditions

Method used

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  • Soft and transparent silk fibroin membrane and preparation method thereof
  • Soft and transparent silk fibroin membrane and preparation method thereof
  • Soft and transparent silk fibroin membrane and preparation method thereof

Examples

Experimental program
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Effect test

Embodiment 1

[0021] This embodiment provides a kind of silk fibroin / urea blend film, and preparation method comprises the following steps:

[0022] 1. Silkworm silk uses 0.5 M NaHCO 3 -Na 2 CO 3 Buffer solution (pH value = 9), boiled repeatedly at 98-100°C for 30 minutes each time, washed with deionized water, and repeated the above operation three times. Dissolve the degummed and dried silk fibers in 9.3M lithium bromide solution at a bath ratio of 1:10 at 60°C, add a double-layer dialysis bag with a molecular weight cut-off of 10,000 Daltons after cooling (the dialysis bag is covered with another Layer dialysis bag, forming a double-layer dialysis bag), dialyzed with deionized water for 3 days to obtain a purified silk fibroin solution.

[0023] 2. Configure 100 mg / mL urea solution, and mix urea and silk fibroin according to the mass ratio (m URE / m SF ): 1.7 / 10 blending, pouring the blended silk fibroin solution into a polystyrene mold, blowing and drying at 25°C and 75% humidity f...

Embodiment 2

[0030] 1. Silkworm silk uses 0.3 M NaHCO 3 -Na 2 CO 3 Buffer solution (pH value = 9), boiled repeatedly at 98-100°C for 30 minutes each time, washed with deionized water, and repeated the above operation three times. Dissolve the degummed and dried silk fiber in 9.3M lithium bromide solution at a bath ratio of 1:10 at 63°C, add a double-layer dialysis bag with a molecular weight cut-off of 10,000 Daltons after cooling (the dialysis bag is covered with another Layer dialysis bag, forming a double-layer dialysis bag), dialyzed with deionized water for 3 days to obtain a purified silk fibroin solution.

[0031] 2. Configure 80 mg / mL urea solution, mix urea and silk fibroin according to the mass ratio (m URE / m SF ): 2 / 10 blending, and then pour the blended silk fibroin solution into a polystyrene mold, blow dry and balance for 48 hours at 25°C and 65% humidity to obtain an insoluble soft transparent silk fibroin film.

[0032]The resulting silk fibroin / urea blend film was te...

Embodiment 3

[0038] 1. Silkworm silk uses 0.7 M NaHCO 3 -Na 2 CO 3 The buffer solution (pH value = 9) was repeatedly boiled at 98-100°C for 28 minutes each time, washed with deionized water, and the above operation was repeated three times. Dissolve the degummed and dried silk fibers in 9.3M lithium bromide solution at a bath ratio of 1:10 at 58°C, add a double-layer dialysis bag with a molecular weight cut-off of 10,000 Daltons after cooling (the dialysis bag is covered with another Layer dialysis bag, forming a double-layer dialysis bag), dialyzed with deionized water for 3 days to obtain a purified silk fibroin solution.

[0039] 2. Configure 300 mg / mL urea solution, and mix urea and silk fibroin according to the mass ratio (m URE / m SF ): 3 / 10 blending, and then pour the blended silk fibroin solution into a polystyrene mold, blow dry and balance at 22°C and 60% humidity for 48 hours to obtain an insoluble soft transparent silk fibroin film. Its dry light transmittance was measured...

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Abstract

The invention discloses a soft and transparent silk fibroin membrane and a preparation method thereof. The preparation method comprises the following steps: taking silkworm fibroin as a base material, blending non-bio-toxic small molecule urea and a silk fibroin aqueous solution, pouring at a room temperature, drying under a certain humidity and balancing into a membrane to prepare the silk fibroin membrane that is soft, is insoluble in water and is kept transparent for a long time in dry and wet states. The light transmittance of the silk fibroin membrane with a thickness of about 100 [mu]m within a visible light range of 400-760 nm in the dry and wet states is 96 percent or above; when the dry-state elongation at break is 75 percent or above, the breaking strength is 25 MPa or above; when the wet-state elongation at break is 120 percent or above, the breaking strength reaches 10 MPa or above. The dissolve-loss ratio in deionized water is 0.5 percent or below. Due to excellent biocompatibility of natural silk fibroin and better oxygen permeability and hydrophilicity of a fibroin membrane, the silk fibroin membrane is particularly suitable for the repair of corneal stroma and the application to contact lens, eye pupil materials and the like.

Description

technical field [0001] The invention relates to a transparent film material and a preparation method thereof, in particular to a soft transparent silk fibroin film and a preparation method thereof. Background technique [0002] Bombyx mori silk fibroin, as a natural protein secreted by Insecta-Lepidoptera during metamorphosis, has excellent biocompatibility and degradability while not carrying pathogens that are common to humans. Silkworm silk fiber has been used in surgical sutures for more than 100 years. In recent decades, with the processing of silk fibroin under mild aqueous solution, a variety of regenerated silk fibroin products have come out, such as silk membranes, micro-nano particles, hydrogels, porous scaffolds, sponges, etc. [0003] The silk fibroin film is made by pouring the regenerated silkworm silk fibroin protein solution on a flat mold and drying and balancing at a certain temperature and humidity, and depositing a solidified protein in film form. While...

Claims

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Application Information

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IPC IPC(8): C08L89/00C08K5/21C08J5/18
CPCC08J5/18C08J2389/00C08K5/21C08L89/00
Inventor 夏婷婷卢神州殷祝平
Owner NANTONG TEXTILE & SILK IND TECH RES INST
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