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Paenibacillus polymyxa, as well as culture method and application thereof

A polymyxa spore-like and bacillus technology, applied in the field of microorganisms, can solve the problem of lack of efficient bacteriostatic substances such as polymyxa spore-like bacillus, and achieve high bacteriostatic effect and obvious inhibitory effect.

Active Publication Date: 2017-05-24
BRIGHT DAIRY & FOOD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] The technical problem to be solved by the present invention is to overcome the lack of Paenibacillus polymyxa capable of producing highly effective bacteriostatic substances in the prior art, and provide a Paenibacillus polymyxa and its cultivation method and application

Method used

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  • Paenibacillus polymyxa, as well as culture method and application thereof
  • Paenibacillus polymyxa, as well as culture method and application thereof
  • Paenibacillus polymyxa, as well as culture method and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0046] Example 1 Isolation of BD3736 strain

[0047] Pick 1 g of pickled cabbage from farmers in Shanghai, take 1 g aseptically, put it in 10 mL of sterile normal saline and mash it, serially dilute it with sterile normal saline, and spread the dilution evenly on the solid TYC medium by coating. , 30 ℃ cultured for 24-48 hours. Multiple single colonies with snot-like shape and good stringiness were selected and transferred to new solid TYC medium to obtain purified colonies, one of which was strain BD3736.

Embodiment 2

[0048] Example 2 Identification of BD3736 strain

[0049] 1. Morphological identification

[0050] The BD3736 strain isolated in Example 1 was inoculated on LB solid medium, LB solid medium containing 0.001% lysozyme, LB solid medium containing 3% NaCl and PDA solid medium, and the percentage was The mass percentage of LB solid medium or the mass of the PDA solid medium. When cultured in an incubator at 30°C for 18h under atmospheric conditions to the logarithmic growth phase and the colony size is stable, the colony morphology is observed, mainly including oxygen utilization, colony size, color, transparency, wetness, colony surface state (whether flat, protruding or not) , folds, depressions, etc.), colony edge status (whether neat, irregular, radial, etc.).

[0051] The results showed that the BD3736 strain was facultative aerobic, did not grow in 0.001% lysozyme or 3% NaCl, and showed a thin layer on the LB solid medium, like the movement and diffusion of amoeba; on the ...

Embodiment 3

[0069] Example 3 Extraction of antibacterial substances derived from Paenibacillus polymyxa BD3736

[0070] 1. Culture of BD3736 strain

[0071] Take the BD3736 strain preserved in 20% glycerol, inoculate it on LB solid medium, and incubate it in an incubator at 30°C for 48h under atmospheric conditions. Then, scrape a ring of single colony into 15 mL of sterilized skim milk with a solid content of 10%, and cultivate at 30° C. and 180 r / min constant temperature shaking for 24 hours to obtain the BD3736 strain seed fermentation broth.

[0072] A 500mL conical flask was filled with 130mL of sterilized skim milk with a solid content of 10%, and the above-mentioned BD3736 strain seed fermentation broth was inoculated according to the inoculum amount of 4% (v / v), and 30 ° C, 180r / min constant temperature vibration for 5 days, The BD3736 strain fermentation broth was obtained.

[0073] 2. Preparation of BD3736 strain fermentation broth extract

[0074] The above-mentioned BD3736 ...

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Abstract

The invention discloses paenibacillus polymyxa, as well as a culture method and an application thereof. The preservation number of the paenibacillus polymyxa is CGMCC No.10062, and the name of a culture is BD3736. The paenibacillus polymyxa disclosed by the invention can generate an antibacterial substance with a broad-spectrum and a high antibacterial effect, and has an obvious inhibitory effect on multiple gram positive bacteria and gram negative bacteria.

Description

technical field [0001] The invention belongs to the technical field of microorganisms, and in particular relates to a Paenibacillus polymyxa and a culture method and application thereof. Background technique [0002] Many microorganisms of the genus Paenibacillus have disease prevention and growth promotion effects on plants, so they have good application potential in agricultural production. Paenibacillus can produce a variety of biologically active substances, such as enzymes, antibacterial substances, plant hormones and flocculants, etc. Most of these active substances are proteins, polysaccharides, polypeptides, etc. [0003] Paenibacillus polymyxa is valued for its advantages of plant disease prevention and plant growth promotion, safety to humans and animals, and no pollution. The US Environmental Protection Agency (EPA) lists it as one of the commercially available microorganisms. The metabolites of Paenibacillus polymyxa contain a variety of available bioactive subs...

Claims

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Application Information

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IPC IPC(8): C12N1/20C12P1/04C12R1/01
Inventor 吴正钧高彩霞刘振民韩瑨鄢明辉冯华峰
Owner BRIGHT DAIRY & FOOD
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