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A biological seed coating agent for preventing and treating zucchini root rot and preparation method thereof

A technology for zucchini and root rot, which is applied in the field of biological seed coating for preventing and treating zucchini root rot and the field of preparation thereof, can solve the problems of harming the natural environment, poor control effect, affecting the quality of zucchini, etc., so as to ensure film-forming stability, Good film-forming properties, preventing pesticide application from exceeding the standard

Active Publication Date: 2019-08-09
VEGETABELE INST AGRI SCI ACAD SHANXI PROV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] In order to solve the problems that the existing prevention and treatment of zucchini root rot caused by Fusarium solani disease affects the quality of zucchini, endangers the natural environment and has poor control effect, the present invention provides a biological seed coating agent for preventing and treating zucchini root rot and its preparation method

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0017] A preparation method of a biological seed coating agent for preventing and treating zucchini root rot, adopting the following steps: a. Bacillus licheniformis is inoculated and cultivated in a PDA medium, the culture temperature is 28° C., and the culture time is 27 hours, and each culture medium is detected The number of live bacteria, the number of bacteria is greater than 10 9 After cfu / mL, carry out expanded culture; b. According to the three-stage culture of slope, seed and liquid fermentation, 1‰ zinc sulfate is added during the liquid fermentation process to form bacitracin zinc, the culture temperature is 27 ° C, and the culture time is 42 hours. Lichen The bacteria content of the bacillus liquid is greater than or equal to 5×10 9 CFU / g; c. After mixing sodium carboxymethylcellulose, polyethylene glycol, gum arabic, pearl powder, and dyeing agent in a ratio of 3:4:4.2:1:1.8, mix them with SW-1 Bacillus licheniformis solution Mix according to mass ratio 1:1.

Embodiment 2

[0019] A preparation method for a biological seed coating agent for preventing and treating zucchini root rot, adopting the following steps: a. Bacillus licheniformis is inoculated and cultivated in a PDA medium, the culture temperature is 27° C., and the culture time is 24 hours, and each culture medium is detected The number of live bacteria, the number of bacteria is greater than 10 9 After cfu / mL, carry out expanded cultivation; b. According to the three-stage cultivation of slope, seed and liquid fermentation, 1‰ zinc sulfate is added in the liquid fermentation process to form bacitracin zinc, the cultivation temperature is 26°C, and the cultivation time is 40 hours. The bacteria content of the bacillus liquid is greater than or equal to 5×10 9 CFU / g; c. After mixing sodium carboxymethylcellulose, polyethylene glycol, gum arabic, pearl powder, and dyeing agent in a ratio of 3:3.5:3:1:1.5, mix them with SW-1 Bacillus licheniformis solution Mix according to mass ratio 1:1....

Embodiment 3

[0021] A preparation method of a biological seed coating agent for preventing and treating zucchini root rot, adopting the following steps: a. Bacillus licheniformis is inoculated and cultivated in a PDA medium, the culture temperature is 35° C., and the culture time is 36 hours, and each culture medium is detected The number of live bacteria, the number of bacteria is greater than 10 9 After cfu / mL, expand the cultivation; b. According to the three-stage cultivation of slope, seed and liquid fermentation, 1‰ zinc sulfate is added in the liquid fermentation process to form bacitracin zinc, the cultivation temperature is 28°C, and the cultivation time is 45 hours. The bacteria content of the bacillus liquid is greater than or equal to 5×10 9 CFU / g; c. After mixing sodium carboxymethylcellulose, polyethylene glycol, gum arabic, pearl powder, and dyeing agent in a ratio of 3:4.5:5:1:2, mix them with SW-1 Bacillus licheniformis solution Mix according to mass ratio 1:1.

[0022] ...

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Abstract

The invention is specifically a biological seed coating agent for zucchini root rot and a preparation method thereof, which solves the problems that the existing prevention and control of zucchini root rot caused by Fusarium solani can affect the quality of zucchini, endanger the natural environment and have poor control effects . A biological seed coating agent for zucchini root rot, comprising the following components: sodium carboxymethylcellulose, polyethylene glycol, gum arabic, bacillus licheniformis liquid; sodium carboxymethylcellulose, polyethylene glycol and gum arabic The mass ratio is 3:3.5‑4.5:3‑5, and the bacterial content of Bacillus licheniformis liquid is greater than or equal to 5×10 9 CFU / g. The biological seed coating agent of the present invention has a strong antagonistic effect, can be used for early prevention and treatment of zucchini root rot, promotes the growth of zucchini plants, greatly improves the quality of zucchini, and has the advantages of good film-forming property and high stability , Put an end to the occurrence of excessive pesticide application and poor agricultural control effect in the current root rot prevention and control process.

Description

technical field [0001] The invention relates to the field of microbial seed coating agents, in particular to a biological seed coating agent for preventing and treating zucchini root rot caused by Fusarium solani and a preparation method thereof. Background technique [0002] Zucchini root rot is mainly caused by Fusarium solani and is a serious soil-borne disease. Because root rot starts at the root of the plant, causing the root to rot, and finally the whole plant dies. In addition, the disease spreads rapidly, and the incidence rate of severely damaged protected areas is 70-90%, which seriously reduces production and income, and hurts the enthusiasm of growers. The benign development of the zucchini industry has been guaranteed. In recent years, growers have mainly adopted measures such as chemical agents and high-temperature stuffy sheds to reduce losses caused by zucchini root rot, but the effect is limited. [0003] At present, zucchini root rot mainly relies on meas...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): A01N63/02A01N59/16A01N43/78A01P3/00A01P21/00A01C1/06C12N1/20C07K7/58C12R1/10
CPCA01C1/06A01N43/78A01N59/16A01N63/10C07K7/58C12N1/20C12N1/205C12R2001/10A01N2300/00
Inventor 申琼武峻新梁宏董晓飞黄静陈哲赵佳侯岗
Owner VEGETABELE INST AGRI SCI ACAD SHANXI PROV
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