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Pharmaceutical composition for treating or preventing inflammatory diseases

A technology of inflammatory bowel disease and composition, which is applied in the field of pharmaceutical composition for the treatment or prevention of inflammatory diseases, and can solve problems such as the unexplained anti-inflammatory mechanism of breviscapella

Active Publication Date: 2017-05-31
SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Although it is used by folks for the treatment of the above diseases, the specific anti-inflammatory mechanism of Brachycarpa brevifolia has not been elucidated

Method used

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  • Pharmaceutical composition for treating or preventing inflammatory diseases
  • Pharmaceutical composition for treating or preventing inflammatory diseases
  • Pharmaceutical composition for treating or preventing inflammatory diseases

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0089] Example 1: Preparation of Sopubia trifida Buch.-Ham.ex D.Don Extract

[0090] 1-1. Preparation of Grass brevis extract

[0091] Grass brevis is collected from the medicinal botanical garden of Shandong University of Traditional Chinese Medicine. Take dried and ground 10kg leaves of breves brevis, extract them with 95% ethanol for 3 times, each time for 5 hours, then filter and take the supernatant . The obtained supernatant was concentrated in vacuo to obtain 0.5 kg of ethanol extract of Brachyphyllum brevifolia.

[0092] 1-2. Preparation of Grass brevis fraction

[0093] The ethanol extract of Grass brevifolia prepared in Example 1-1 was suspended in 1 L of water, further mixed with 1 volume of 100% petroleum ether, and left to stand while stirring. This process was performed twice while removing the aqueous layer. As a result, 1.8 g of petroleum ether extract was obtained. After removing the petroleum ether fraction, an equal volume of chloroform was added and st...

Embodiment 2

[0094] Example 2: Inhibitory Activity Test of Grass brevis Extracts and Fractions on Cell Growth and NO Generation

[0095] 2-1. Cell viability test

[0096] Mouse macrophage J774.1 was selected as the test cell, and the effect of different concentrations of Brachycarpa extract on cell viability was detected. More specifically, mouse macrophage J774.1 was treated with 1x10 6 Cells / ml were suspended in phenol red-free DMEM supplemented with 5% fetal bovine serum, and then the suspension was seeded in a 96-well cell culture plate at 80 μL / well. Four hours later, the adherent cells were incubated with each fraction for 24 hours and then incubated with 10 μL per well of 5 mg / ml MTT solution for an additional 4 hours. After removing the medium, add 100 μL of DMSO to each well and read the absorbance at 550 nm. Cell viability was expressed as a percentage of the absorbance at 550 nm relative to the negative control treated with 0.1% DMSO, calculated according to a mathematical for...

Embodiment 3

[0110] Example 3: Anti-inflammatory activity of Grass brevis extracts in J774.1 cells

[0111] 1-1. Inhibitory activity against NF-κB

[0112] The translocation of NF-κB into the nucleus of J774.1 cells under LPS-induced inflammatory conditions was detected by immunofluorescence. For this, J774.1 cells were grown at 1x10 6 Cells / ml were suspended in DMEM containing phenol red and 10% fetal bovine serum, seeded onto plastic chamber slides, and allowed to attach for 8 hours to the slide. Subsequently, the cells were incubated with 50 μg / mL of each Brachycarpa extract for 2 hours, and then further incubated with 1 μg / mL lipopolysaccharide (LPS, Sigma) for 1.5 hours. After removing the medium, the cells were fixed with ethanol for 30 min at 4°C, washed with phosphate-buffered saline, and blocked with 5% bovine serum albumin for 40 min at room temperature. Then, cells were reacted with primary antibody [anti-NF-κB antibody (1:100)] for 5 hours at room temperature, washed thoroug...

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Abstract

The invention discloses a pharmaceutical composition for treating or preventing inflammatory diseases. The pharmaceutical composition takes extract from sopubia stricta as an active ingredient. The extract can further inhibit expression f iNOS and COX-2 by inhibiting generation of NO and PGE2 in inflammatory reaction and inhibiting NF-kB transposition to nuclear so as to play a role of preventing inflammation (in particular allergic rhinitis). The pharmaceutical composition has a good effect in both in-vivo and in-vitro tests.

Description

technical field [0001] The present invention relates to a pharmaceutical composition for preventing and treating inflammatory diseases and its application for treating inflammatory diseases. The specific pharmaceutical composition contains Grass brevis extract as an active ingredient. Background technique [0002] Inflammation is the body's defense response to stimuli, manifested as redness, swelling, heat, pain, and dysfunction. It can be infectious inflammation due to infection or non-infectious inflammation not due to infection. Inflammatory signals are produced through cyclooxygenase (COX) pathway and lipoxygenase (LOX) pathway, and prostaglandins, leukotrienes, thromboxanes, etc. are produced through these pathways. In the inflammatory process, on the one hand, the damage factors directly or indirectly cause tissue and cell destruction, and on the other hand, the damage factors are diluted, killed and surrounded by inflammatory hyperemia and exudative reactions. At th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K36/80A61P29/00A61P11/00A61P11/02A61P37/08A61P19/08A61P19/02A61P1/00
CPCA61K36/80A61K2236/33A61K2236/331
Inventor 韩秀丽谭智敏宁云红
Owner SHANDONG UNIV OF TRADITIONAL CHINESE MEDICINE
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