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Metro engineering concrete containing microbial capsules and capable of realizing continuous self-repairing

A technology of microorganisms and concrete, applied in the direction of being fixed on or in an inorganic carrier, or being fixed on or in an organic carrier, etc., can solve the problems of poor adhesion, influence of strength, easy to be washed away, etc. The effect of strong performance, large adsorption capacity and good compressive strength

Inactive Publication Date: 2017-05-31
UNIV OF JINAN
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Its mechanism is that the crack penetrates the composite capsule, and the microorganisms are released to metabolize to generate carbon dioxide to react with calcium ions to form calcium carbonate to repair the crack. Second, the capsule can only be used once, and cannot continuously repair the cracks at the same position in the concrete; third, the outer surface of the capsule is a smooth structure, which has poor adhesion to the concrete, and it is easy to Washed away, unable to repair

Method used

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  • Metro engineering concrete containing microbial capsules and capable of realizing continuous self-repairing

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0027] Preparation of microbial capsule:

[0028] Step 1. Cultivate Bacillus lysine and get the sludge after centrifugation. The sludge concentration is 6×10 9 Pcs / ml;

[0029] Step 2. Prepare anhydrous Bacillus lysine, tryptone 510g, soy peptone 90g, glucose 75, NaCl150g; dipotassium hydrogen phosphate 25g;

[0030] Step 3: Add all the culture medium and 0.1ml of bacterial mud configured in step 2 to 1200ml of water and stir to obtain a mixed solution;

[0031] Step 4. After adding 100 g of macroporous adsorption resin to the mixture obtained in step 3, stir at 250r / min for 30 minutes, filter and collect the macroporous adsorption resin, and bake at 20°C until the water content is less than 5%;

[0032] Step 5. Put the dried macroporous adsorption resin in 90% wt alcohol for the first soak for 30 minutes, and spray the cement liquid with a mass fraction of 20% on the surface uniformly to make the thickness 0.3mm Dry at ℃, spray again a mixture of cement and calcium chloride in a mass ...

Embodiment 2

[0036] Preparation of microbial capsule:

[0037] Step 1. Cultivate Bacillus lysine or Bacillus cereus, and obtain bacterial sludge after centrifugation, with a concentration of 6×10 9 Pcs / ml;

[0038] Step 2. Prepare anhydrous aerobic alkalophilic Bacillus culture medium, tryptone 510g, soy peptone 90g, glucose 75, NaCl150g; dipotassium hydrogen phosphate 25g;

[0039] Step 3: Add all the medium and 0.1ml of bacteria mud configured in Step 2 to 1500ml of water and stir to obtain a mixed solution;

[0040] Step 4. After adding 100 g of macroporous adsorption resin to the mixture obtained in step 3, stir at 250r / min for 20 minutes, filter and collect the macroporous adsorption resin, and bake at 30°C until the water content is less than 5%;

[0041] Step 5. Put the dried macroporous adsorption resin in 100% wt alcohol and soak it for 50 minutes once, and spray 25% cement liquid evenly on the surface to make the thickness 0.8mm and dry at 25°C , Spray the mixture of cement and calcium ch...

Embodiment 3

[0044] Preparation of microbial capsule:

[0045] Step 1. Cultivate Bacillus cereus, and get the sludge after centrifugation. The sludge concentration is 4×10 9 Pcs / ml;

[0046] Step two, configure anhydrous M.S.P medium, NaNO 3 9g, K 2 HPO 4 3g, KCI1.5g, MgSO 4 1.5g, FeSO 4 , 0.03g, 90g of sucrose;

[0047] Step 3: Add all the culture medium and 0.1ml of bacterial mud configured in step 2 to 180ml of water and stir to obtain a mixed solution;

[0048] Step 4. After adding 15 g of macroporous adsorption resin to the mixture obtained in step 3, stir for 30 min at a speed of 250r / min, filter and collect the macroporous adsorption resin, and bake at 30°C until the water content is less than 5%;

[0049] Step 5. Put the dried macroporous adsorption resin into 100%wt alcohol and soak it for 40min once, and spray 25% cement liquid evenly on the surface to make the thickness 0.5mm, and dry it at 30℃ Dry in a box, spray again a mixture of cement and calcium chloride with a mass ratio of 2:1, t...

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Abstract

The invention discloses metro engineering concrete containing microbial capsules and capable of realizing continuous self-repairing and relates to the technical field of concrete repairing. The concrete is C30 concrete prepared from raw materials in parts by weight as follows: 266 parts of cement, 114 parts of fly ash, 718 parts of sand, 592 parts of finely broken stones, 484 parts of small broken stones, 157 parts of water, 33 parts of an HLC anti-crack and anti-seepage agent and 15-20 parts of microbial capsules. The concrete can replace workers to solve the problem of underground engineering seepage in a longer time and has better repairing power up to 99%. Therefore, the concrete has higher economic benefits and market potential.

Description

Technical field [0001] The invention relates to the technical field of concrete repairing, in particular to a subway engineering concrete containing microbial capsules capable of continuous self-repairing. Background technique [0002] The treatment of cracking of existing concrete structures is a common technical problem in the entire civil engineering field. Every country in the world has a limit on the allowable crack width. For example, the technical standards of the United States and New Zealand allow a crack width of 0.4mm (under dry conditions), and 0.2~0.3mm in my country. The appearance of cracks affects the safety and applicability of the structure. If there are cracks in underground works, water seepage will occur, which will seriously affect the use of underground works; and when the beams, columns, walls, etc. of office buildings and houses appear cracks, they will not only affect the appearance and life of the building, but also threaten people's lives and property...

Claims

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Application Information

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IPC IPC(8): C04B28/00C04B24/00C04B20/12C12N11/14C12N11/02
CPCC12N11/02C12N11/14C04B20/12C04B24/00C04B28/00C04B2201/50C04B18/08C04B14/06C04B14/02C04B2103/0068C04B2103/0001C04B20/1077C04B20/107
Inventor 唐禄博张华玮赵慷慨林凡通李壮壮王丽丽王鹏飞
Owner UNIV OF JINAN
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