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Fucoidan sulfate oligosaccharide and its preparation method and application

A technology of fucoidan sulfate and fucoidan sulfate, which is applied in the field of fucoidan sulfate oligosaccharides and its preparation and application, can solve the problem of small application range, limited number of fucose residues and Types and other issues, to achieve the effect of enriching types, expanding the scope of use, and high yield

Active Publication Date: 2019-09-17
BIOLOGY INST OF SHANDONG ACAD OF SCI +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, disaccharides with structures A and B are limited by the number and types of fucose residues in the molecule, and their application range is small

Method used

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  • Fucoidan sulfate oligosaccharide and its preparation method and application
  • Fucoidan sulfate oligosaccharide and its preparation method and application
  • Fucoidan sulfate oligosaccharide and its preparation method and application

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0036] Put the recombinant fucoidanase FFA1 (1mg) into the Tris-HCl buffer solution with a pH value of 7.2, mix it with 5% copper algae (Sargassum horneri) fucoidan solution, and put the mixture at a temperature of 37°C Artificially cultivated for 72 hours, and then heated to 100°C for 5 minutes. The resulting precipitate was separated by centrifugation and discarded. Add ethanol to the supernatant until its concentration reaches 75%. The formed precipitate (polymer component) was centrifuged at 9000 g for 20 min. The supernatant (low molecular components) was poured into a column containing Q-Sepharose adsorbent, and equilibrated with pure water. The oligosaccharides were then eluted with a linear gradient of ammonium bicarbonate at a rate of 1 mL / min. First operate to obtain sulfated fucosylated oligosaccharides with molecular formula II, then operate to obtain sulfated fucosylated oligosaccharides with molecular formula I, and then freeze-dry the obtained product to obta...

Embodiment 2

[0038] Put the recombinant fucoidanase FFA1 (1mg) into the Tris-HCl buffer solution with a pH value of 7.2, mix it with 10% copper algae (Sargassum horneri) fucoidan solution, and put the mixture at a temperature of 37°C Artificially cultivated for 75 hours, and then heated to 100°C for 10 minutes. The resulting precipitate was separated by centrifugation and discarded. Add acetone to the supernatant until its concentration reaches 75%. The formed precipitate (macromolecule component) was centrifuged at 10000 g for 30 min. The supernatant (low molecular components) was poured into a column containing Q-Sepharose adsorbent, and equilibrated with pure water. Then, the oligosaccharides were eluted with a linear gradient of ammonium bicarbonate at a speed of 1 mL / min. First operate to obtain the fucoidan sulfate oligosaccharide with molecular formula II, then operate to obtain the fucoidan sulfate oligosaccharide with molecular formula I, then freeze-dry the obtained product, a...

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Abstract

The invention discloses novel fucoidan oligosaccharides as well as a preparation method and application thereof. The novel fucoidan oligosaccharides comprises a chain formed by connecting four fucose residues in sequence, wherein the fucose residues are alternately chemically combined from 1 to 3 and 1 to 4; the sulfate radical is distributed on C2 and C3 of the second fucose residue and C2 of the third fucose residue. The quantity of the fucose residues (fixed structures) in the fucoidan oligosaccharides provided by the invention is equal to 4 or 6, so that the amount of the fucoidan oligosaccharides with the fixed structures is enlarged, and the use range of the fucoidan oligosaccharides is expanded; more component sources are supplied to medicines for preventing or treating atopic dermatitis and eczema and gastrointestinal infection.

Description

technical field [0001] The invention specifically relates to a fucoidan sulfate ester oligosaccharide, a preparation method and application thereof. Background technique [0002] Fucoidan is a sulfated heteropolysaccharide of fucoidan with broad-spectrum biological activity [Fitton, J.H., Stringer, D.N., and Karpiniec, S.S. (2015). Therapies from Fucoidan: An Update. Marin Drugs 13, 5920-5946. ]. However, since polysaccharide preparations are heterogeneous and polysaccharide preparations cannot be standardized, it is almost impossible to use polymer molecules in medicine. Therefore, how to extract oligosaccharides with fixed structures and standard properties from fucoidan has become a difficult problem. The use of enzymes to depolymerize fucoidan and extract bioactive oligosaccharides provides new possibilities for the use of such compounds in pharmacy and cosmetic surgery [Kusaykin, M.I., Silchenko, A.S., Zakharenko, A.M. , and Zvyagintseva, T.N. (2016). Fucoidanases. G...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07H11/00A61K31/7024A61K31/737A61P17/00A61P29/00A61P1/00C12P19/04C12P19/14
Inventor 米哈伊尔·库萨金刘昌衡张绵松袁文鹏贾爱荣赵佩佩张玉史亚萍夏雪奎阿提姆·西尔琴科安东·瑞森阿纳托利·卡利诺夫斯卡斯维特拉娜·厄玛克娃塔吉亚娜·兹维亚金特斯娃
Owner BIOLOGY INST OF SHANDONG ACAD OF SCI
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