Method for extracting Nostoc sphaeroids Kutz protein
An extraction method, the technology of Gexianmi, applied in the preparation method of peptides, chemical instruments and methods, organic chemistry, etc., can solve the problems of not taking into account the protein of Gexianmi, consuming a lot of manpower, material and financial resources, and difficult to guarantee the purity of the product. , to achieve the effect of easy realization and storage, simplified operation difficulty, and avoid easy deterioration
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Image
Examples
experiment example 1
[0092] Experimental example 1 small laboratory test to extract phycobiliprotein
[0093] Grind the dried or dried kudzu rice (water content 14.6%) with a universal grinder to more than 10-200 mesh, pass through a sieve, and obtain grains with a number of 10-24 mesh and 24-65 mesh , 65-80 mesh, 80-120 mesh, 120-200 mesh and more than 200 mesh, take 0.5g respectively, add 25mL purified water, extract at 30°C for 6h, centrifuge at 4000r / min for 10min, filter to obtain crude extract. The results are shown in Table 1 below and figure 1 ,Depend on figure 1 It can be seen that the number of grains of the best kudzu rice noodles is 120 mesh to 200 mesh.
[0094] Table 1
[0095]
experiment example 2
[0096] Experimental example 2 small laboratory test to extract phycobiliprotein
[0097] Use a universal grinder to pulverize the dried or dried Gexian rice (water content 14.6%) to 120-200 mesh, take 0.5g respectively, add 25mL of purified water, add water to the Gexian rice powder and dissolve it by ultrasonic for 30 minutes, After 1 hour of extraction, ultrasonication was performed for 10 min, 20 min, 30 min, 40 min, 50 min, and 60 min. After ultrasonication, it was centrifuged at 4000 r / min for 10 min and filtered to obtain a crude extract. The results are shown in Table 2 below and figure 2 ,Depend on figure 2 It can be seen that with the prolongation of ultrasonic time, the extraction rate of phycobiliprotein of Gexianmi is basically the same. Considering comprehensively, the optimal ultrasonic time is 20 minutes.
[0098] Table 2
[0099]
experiment example 3
[0100] Experimental example 3 small laboratory test to extract phycobiliprotein
[0101]Use a universal grinder to pulverize the dried or dried kudzu rice (water content 14.6%) to 120-200 mesh, take 0.5g respectively, add 25mL of purified water, extract for 1h, and then ultrasonicate for 20min at a power of 400W , 500W, 600W, 700W, 800W and 900W, centrifuge at 4000r / min for 10min after ultrasonication, and filter to obtain crude extract. The results are shown in Table 3 below and image 3 ,Depend on image 3 It can be seen that the best ultrasonic power is 700W.
[0102] table 3
[0103]
PUM
| Property | Measurement | Unit |
|---|---|---|
| particle size (mesh) | aaaaa | aaaaa |
Abstract
Description
Claims
Application Information
Login to View More