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Reactive oxygen photobioreactor for microalgae culture

A technology for photobioreactor and microalgae cultivation, applied in the field of active oxygen photobioreactor, can solve problems such as toxicity, environmental animal hazards, and difficulty in application

Inactive Publication Date: 2017-05-31
BIOALGO CY CO LTD
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, methylene blue is toxic and will cause harm to the environment, animals, and human body, and it is difficult to apply it in large-scale production

Method used

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  • Reactive oxygen photobioreactor for microalgae culture
  • Reactive oxygen photobioreactor for microalgae culture
  • Reactive oxygen photobioreactor for microalgae culture

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] as attached figure 1 As shown, a kind of reactive oxygen column type photobioreactor for microalgae culture provided by the present invention comprises column type photobioreactor 1, photocatalyst inner tube device 2, air inlet 3, air outlet 4, liquid inlet Port 5, liquid outlet 6, gas distributor 7, light source 8, photocatalyst coating 9, reactor top cover 10, power cord 11, light intensity controller 12.

[0025] Column photobioreactor 1 is a cylindrical structure made of plexiglass, with a height of 150 cm and an inner diameter of 30 cm. A gas distributor 7 is installed at the bottom. The gas distributor 7 is connected to the air inlet 3, and the liquid outlet 6 is located at the reaction bottom of the device. The photocatalyst inner tube device 2 is made of a transparent quartz glass tube with a closed bottom and an open top. The light intensity controller 12 is connected, and the outside of the quartz glass tube is evenly coated with a transparent photocatalyst ...

Embodiment 2

[0028] as attached figure 2 As shown, a kind of active oxygen plate-type photobioreactor for microalgae culture provided by the present invention has an overall structural design as in Example 1, the difference is that the reactor 1 is made of transparent glass, and its length, width and height are respectively 150 cm, 20 cm, 120 cm, 3 groups of photocatalyst inner tube devices 2 with a length of 70 cm and an inner diameter of 10 cm are glued to the bottom of the glass top cover 10 of the photobioreactor with glass glue.

[0029] The inoculation operation of Haematococcus pluvialis was the same as in Example 1, the inoculation concentration was 0.22 g / L, and the light intensity of the built-in light source 5 was controlled by the light intensity controller 12 at 50-100 µmol photon∙m-2∙s-1. After reactor 1 was placed outdoors for 10 days, the astaxanthin content (w / w) of the algae cells reached 3.02.%, and the cell biomass reached 0.86 g / L. Reactor, astaxanthin production inc...

Embodiment 3

[0031] as attached image 3 As shown, the present invention provides a reactive oxygen pipeline type photobioreactor for microalgae cultivation, the overall structure design is as in Example 1, the difference is that the reactor 1 is made of stainless steel, with a height of 100 cm and an inner diameter of 40 cm. Two sets of photocatalyst inner tube devices 2 with a length of 50 cm and an inner diameter of 10 cm are fixed under the stainless steel top cover 10 of the photobioreactor with flanges. Reactor 1 was connected to a glass pipe with an inner diameter of 5 cm, and a diaphragm pump was used for algal fluid circulation.

[0032] The inoculation operation of Haematococcus pluvialis is the same as that in Example 1, the inoculation concentration is 0.56 g / L, and the built-in light source 5 is controlled by the light intensity controller 12 at 80-200 μmol photon∙m -2 ∙s -1. After reactor 1 was cultured outdoors for 10 days, the astaxanthin content (w / w) of the algae cells...

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Abstract

The invention relates to a reactive oxygen photobioreactor for microalgae culture and belongs to the technical field of microalgae biology. The reactive oxygen photobioreactor comprises an inner cylinder for generation of reactive oxygen, a light source and a photocatalyst coating, the light source is arranged on the inner side of the inner cylinder, and the photocatalyst coating is coated with transparent water on the outer side. Water and oxygen in an algae solution can be catalytically generated into active oxygen through the photocatalyst, generation control on the active oxygen is regulated through light, synthesis of target metabolites of algae cells is facilitated, and growth of other invasive species is restrained; as a catalyst, the photocatalyst cannot be consumed during culture, adherent growth of algae can be avoided due to hydrophobic nature of the photocatalyst, disinfection treatment on the photobioreactor and culture medium can be performed before culture through the active oxygen, and compared with a traditional photobioreactor, the reactive oxygen photobioreactor has remarkable advantages.

Description

technical field [0001] The invention relates to the field of microalgae biotechnology, and in particular provides an active oxygen photobioreactor for cultivating microalgae. Background technique [0002] Haematococcus pluvialis is a freshwater single-celled green alga belonging to Chlorophyta, Chlorophyceae, Volvox, Haematococcus, and Haematococcus. Haematococcus pluvialis is called Haematococcus pluvialis or Haematococcus pluvialis because it can accumulate a large amount of astaxanthin and make the algae appear red. It is considered to be the best biological platform for producing astaxanthin in nature. The natural astaxanthin produced by Haematococcus pluvialis has good quality and strong antioxidant activity. Haematococcus pluvialis usually exists in the form of green vegetative cells in an environment with weak light and rich nitrogen and phosphorus, which rapidly divides and reproduces, and the content of astaxanthin in the cells is low; under stress conditions such ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12M1/36C12M1/12C12M1/04C12M1/02C12M1/00
CPCY02A90/40C12M21/02C12M23/38C12M29/06C12M31/10C12M37/00C12M41/06
Inventor 代云法李倩郑玉瑞王华郑玉彬
Owner BIOALGO CY CO LTD
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