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Preparation method of earthworm polypeptide extract

An extract and earthworm technology, which is applied in the field of polypeptide preparation, can solve the problems of low polypeptide extraction rate, poor color, fishy smell and bitter taste, etc., and achieves the effects of improving extraction rate, quality and good antioxidant activity.

Inactive Publication Date: 2017-05-31
WEIFANG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] At present, the preparation of earthworm active polypeptides mainly consists of the following methods: (1) preparing earthworm active small molecular polypeptides by enzymatic hydrolysis of the autolytic enzymes contained in earthworms themselves; (2) by adding exogenous enzymes, and then combining endogenous Enzyme hydrolysis to obtain earthworm polypeptide extract; however, the above preparation method has strict requirements on pH value and temperature, and the obtained polypeptide has low extraction rate, poor color, fishy smell and bitter taste

Method used

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Embodiment 1

[0025] A preparation method of earthworm polypeptide extract, said preparation method comprising the following steps:

[0026] (1) Pretreatment: Take 1000g of fresh earthworms and clean them, then soak them in 0.5mg / L sucrose solution for 20 minutes, then remove them, then soak them in normal saline for 15 minutes, until there is no sediment in the normal saline Remove and drain the earthworms, then add 0.15 mol / L potassium chloride solution according to the ratio of solid to liquid 1:10, homogenate, and ultrasonically extract (ultrasonic power 80w, ultrasonic time 10min, ultrasonic temperature 30°C) to obtain solid-liquid mixture;

[0027] (2) Fermentation treatment: get the solid-liquid mixture in step (1), add 0.05 times the mixed strain of solid-liquid mixture weight (the mass ratio of lactic acid bacteria, yeast and aspergillus is 0.1:0.15:0.12), adjust pH 3.5, temperature controlled at 30°C for 12 hours of fermentation to obtain a fermentation mixture;

[0028] (3) One...

Embodiment 2

[0033] A preparation method of earthworm polypeptide extract, said preparation method comprising the following steps:

[0034] (1) Pretreatment: Take 1000g of fresh earthworms and clean them, then soak them in 0.8 / L sucrose solution for 25 minutes, then take them out, then place the earthworms in normal saline and soak them for 20 minutes. The earthworms were fished out and drained, then 0.2mol / L potassium chloride solution was added according to the ratio of solid to liquid 1:15, homogenized, and ultrasonically extracted (ultrasonic power 120w, ultrasonic time 15min, ultrasonic temperature 35°C) to obtain solid liquid mixture;

[0035] (2) Fermentation treatment: get the solid-liquid mixture in step (1), add the mixed strain (the mass ratio of lactic acid bacteria, saccharomycete and aspergillus is 0.2:0.27:0.2) of 0.1 times of the solid-liquid mixture weight, adjust pH 4.5, temperature controlled at 32°C for 18 hours of fermentation to obtain a fermentation mixture;

[003...

Embodiment 3

[0041] A preparation method of earthworm polypeptide extract, said preparation method comprising the following steps:

[0042](1) Pretreatment: Get 1000g of fresh earthworms and clean them up, then soak them in 1mg / L sucrose solution for 35 minutes, then take them out, then put the earthworms in normal saline and soak them for 25 minutes. The earthworms were fished out and drained, and then 0.25mol / L potassium chloride solution was added according to the ratio of solid to liquid 1:18, homogenized, and ultrasonically extracted (ultrasonic power 160w, ultrasonic time 15min, ultrasonic temperature 40°C) to obtain solid liquid mixture;

[0043] (2) Fermentation treatment: get the solid-liquid mixture in step (1), add 0.1 times the mixed strain of solid-liquid mixture weight (the mass ratio of lactic acid bacteria, yeast and aspergillus is 0.43:0.3:0.25), adjust pH 5.5, temperature controlled at 35°C for 20 hours of fermentation to obtain a fermentation mixture;

[0044] (3) Prim...

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Abstract

The invention relates to a polypeptide preparation method, and particularly relates to a preparation method of earthworm polypeptide extract, the preparation method of the earthworm polypeptide extract includes (1) pretreatment, to be more specific, taking fresh earthworms, washing clean, homogenizing, and performing ultrasonic extraction; (2) fermentation treatment, to be more specific, adding mixed strains into a solid-liquid mixture, adjusting pH, and fermenting; (3) first enzymolysis, to be more specific, adding bromelain into the fermentation mixture, adjusting pH, and inactivating enzymes to obtain an enzymolysis substance; (4) second enzymolysis, to be more specific, adding pepsin into the enzymolysis substance for enzymolysis treatment, centrifuging the enzymolysis substance for removal of slag, and collecting supernatant; and (5) ultrafiltration treatment, to be more specific, taking the supernatant collated by the step (4) for ultrafiltration with an ultrafiltration membrane, and using a nanofiltration membrane for interception of the ultrafiltered filtrate, and lyophilizing the obtained interception liquid to obtain earthworm peptides. The earthworm polypeptide extract prepared by combination of the fermentation and the enzymolysis has good color, no fishy odor and bitter taste, and has better antioxidant activity, and the method also greatly improves the extraction rate of the polypeptides.

Description

technical field [0001] The invention relates to a method for preparing a polypeptide, in particular to a method for preparing an earthworm polypeptide extract. Background technique [0002] Active peptides widely exist in nature, and many active substances in organisms exist in the form of peptides. Without peptides, there will be no activity and no life. Scientists have discovered functional peptides with immune regulation, hormone regulation, enzyme regulation, antiviral, blood pressure lowering and blood fat lowering. Due to the special functions of functional peptides, people's demand for them has increased. Now a variety of functional peptides have been extracted and used for medical and health care. For example, functional soybean protein peptides have been used to reduce blood lipids. [0003] Earthworms are oligochaetes of the annelid phylum, which can improve soil, promote agricultural production, and play special roles in material circulation, biodiversity, and ec...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P39/00C12P21/06C07K1/34
CPCC07K1/34C12P21/06C12P39/00
Inventor 庄宝祥
Owner WEIFANG MEDICAL UNIV
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