A method for detecting dopamine using 5-hydroxytryptamine-gold nanoparticles

A gold nanoparticle and serotonin technology, which is applied in the measurement of color/spectral properties, etc., can solve the problems of inability to detect dopamine, complicated operation of electrochemical methods, complicated pretreatment of probes, etc., and achieves saving action time, rapid detection, and easy operation. Effect

Active Publication Date: 2019-07-19
YANCHENG INST OF TECH
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, electrochemical methods are mostly used for the detection of dopamine, and the operation of electrochemical methods is relatively cumbersome, requiring electrolytic cells, cell bodies, working electrodes, counter electrodes, and reference electrodes, etc.
Or use a chemical sensor containing gold nanoparticles to detect dopamine, but this detection method requires the surface of the gold nanoparticles to be modified by two types of functional small molecules, and the pretreatment of the probe in this step is too complicated to be performed quickly. , simple detection of dopamine

Method used

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  • A method for detecting dopamine using 5-hydroxytryptamine-gold nanoparticles
  • A method for detecting dopamine using 5-hydroxytryptamine-gold nanoparticles
  • A method for detecting dopamine using 5-hydroxytryptamine-gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Slowly add 24.3 μL of 100 mM serotonin solution to 29.2 μL of 144.2 mM HAuCl 4 ·3H 2 O, stir and mix evenly to obtain the first mixed solution. It was then diluted with 7.95 mL of deionized water. Then, at a stirring speed of 55r / min, 0.2mL of NaBH with a mass concentration of 1% 4 Add dropwise to the first mixed solution to obtain a second mixed solution. After 10 minutes, the color of the second mixed solution turned into wine red, indicating that 5-HT-gold nanoparticles were generated. The second mixed solution was placed in the dark and continued to stir for 60mins. The second mixed solution was centrifuged at a centrifugal speed of 6500 rpm for 4 minutes, and the upper liquid was removed to obtain serotonin-gold nanoparticles. Transmission electron microscopy showed that the prepared 5-hydroxytryptamine-gold nanoparticles had a size of 7nm.

[0054] With a pipette gun, 100 μL of the prepared 15 mM 5-hydroxytryptamine-gold nanoparticle solution was extracted a...

Embodiment 2-6

[0061] Embodiment 2-6, the operation method is consistent with embodiment 1, and the operation conditions of incubation time, incubation temperature, 5-hydroxytryptamine-gold nanoparticle solution volume, hydrogen peroxide solution concentration, and TMB solution concentration are consistent with embodiment 1, Jinyuan The molar ratio of the solution to the serotonin solution is 1.3:1, and the pH value of the buffer is changed, and the pH values ​​adopted are 3.5, 5.5, 4.0, 4.5, 5.0. According to different pH values, there are different intensity absorbance at 652nm for analysis Figure 6 .

[0062] see Figure 6It can be seen that when the pH value of the buffer solution ranges from 3.5 to 5.5, when the pH increases, the absorption intensity of the system (a) without dopamine and the system (b) with dopamine decrease gradually, while the change of UV absorbance Rate (c) increases first and then decreases. The change rate of UV absorbance (c) is the largest when the pH is 4....

Embodiment 7-11

[0064] Compared with Example 1, Examples 7-11 only changed the incubation time, the molar ratio of gold source solution to 5-hydroxytryptamine solution was 1.2:1, and the rest of the operating conditions and operating methods remained unchanged. The incubation times adopted in Examples 7-11 were 30 minutes, 5 minutes, 10 minutes, 15 minutes, and 25 minutes in sequence. According to different incubation time settings, the absorbance at 652nm is analyzed with different intensities Figure 7 .

[0065] see Figure 7 It can be seen that the range of incubation time is 5-30mins. With the increase of reaction time, there is no dopamine in the system (a) and dopamine exists in the system (b). Decrease after increasing, when the incubation time is 10mins, the three reach the maximum; the change rate of UV absorbance (c) is the lowest at 30 minutes and 5 minutes, and the UV absorbance is the lowest at 15 minutes in the system with dopamine (b) .

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Abstract

The invention relates to the field of chemical testing and analysis, in particular to a method for detecting dopamine by using 5-serotonin-gold nanoparticles. Mainly including the preparation of 5-HT-Au nanoparticles, 5-HT-Au nanoparticles-Dopamine-TMB-H 2 o 2 System preparation and UV spectrophotometer detection and analysis. The system can produce a special ultraviolet absorption peak at around 652nm, thereby rapidly detecting dopamine. The detection method does not need to take too many complex treatments for the gold nanoparticles, and its operation is simple, fast and time-consuming.

Description

technical field [0001] The invention relates to the field of chemical testing and analysis, in particular to a method for detecting dopamine by using 5-hydroxytryptamine-gold nanoparticles. Background technique [0002] Gold nanoparticles refer to tiny particles of gold with a diameter of 1-100nm. They have high electron density, dielectric properties and catalytic effects, and can combine with various biological macromolecules without affecting their biological activity. Gold nanoparticles of various particle sizes can be conveniently prepared by the reduction method from chloroauric acid, and its color is red to purple depending on the diameter. [0003] Dopamine is a catecholamine neurotransmitter present in the central nervous system of mammals, and changes in its amount can lead to some important diseases such as schizophrenia and Parkinson's disease. At present, electrochemical methods are mostly used to detect dopamine, and the operation of electrochemical methods is...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 姜翠凤李朝吴辉朱佳茗朱冬芝
Owner YANCHENG INST OF TECH
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