Method for detecting dopamine by utilizing 5-hydroxytryptamine-gold nanoparticles

A technology of gold nanoparticles and serotonin, which is applied in the measurement of color/spectral characteristics, etc., can solve the problems of cumbersome electrochemical methods, inability to detect dopamine, and complex pretreatment of probes, and achieve time-saving, rapid detection, and easy operation Effect

Active Publication Date: 2017-05-31
YANCHENG INST OF TECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, electrochemical methods are mostly used for the detection of dopamine, and the operation of electrochemical methods is relatively cumbersome, requiring electrolytic cells, cell bodies, working electrodes, counter electrodes, and reference electrodes, etc.
Or use a chemical sensor containing gold nanoparticles to detect dopamine, but this detection method requires the surface of the gold nanoparticles to be modified by two types of functional small molecules, and the pretreatment of the probe in this step is too complicated to be performed quickly. , simple detection of dopamine

Method used

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  • Method for detecting dopamine by utilizing 5-hydroxytryptamine-gold nanoparticles
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  • Method for detecting dopamine by utilizing 5-hydroxytryptamine-gold nanoparticles

Examples

Experimental program
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Effect test

Embodiment 1

[0053] Slowly add 24.3μL 100mM serotonin solution to 29.2μL 144.2mM HAuCl 4 ·3H 2 In O, stir and mix uniformly to obtain the first mixed solution. It was then diluted with 7.95 mL deionized water. Then, under a stirring speed of 55r / min, 0.2mL of NaBH with a mass concentration of 1% 4 Add dropwise to the first mixed solution to obtain a second mixed solution. After 10 minutes, the color of the second mixed solution changed to wine red, indicating that serotonin-gold nanoparticles were formed. The second mixed solution was placed in the dark and stirred for 60 minutes. The second mixed solution was centrifuged at a centrifugal speed of 6500 rpm for 4 minutes, and the upper liquid was removed to obtain serotonin-gold nanoparticles. Transmission electron microscopy showed that the size of the serotonin-gold nanoparticles prepared was 7 nm.

[0054] Use a pipette to extract 100 μL of the prepared 15mM 5-hydroxytryptamine-gold nanoparticle solution and add them to 9 plastic EP tube...

Embodiment 2-6

[0061] Example 2-6, the operation method is the same as that of Example 1. The operating conditions of incubation time, incubation temperature, serotonin-gold nanoparticle solution volume, hydrogen peroxide solution concentration, and TMB solution concentration are the same as in Example 1. Gold source The molar ratio of the solution to the serotonin solution is 1.3:1, and the pH value of the buffer is changed to 3.5, 5.5, 4.0, 4.5, and 5.0. According to the setting of different pH values, there are different intensity absorbance at 652nm for analysis. Image 6 .

[0062] See Image 6 It can be seen that when the pH value of the buffer solution changes in the range of 3.5-5.5, when the pH increases, the absorption intensity of the system without dopamine (a) and the system with dopamine (b) gradually decreases, while the UV absorbance changes The rate (c) first increases and then decreases. When the pH is 4.2, the UV absorbance change rate (c) is the largest, and the UV absorpti...

Embodiment 7-11

[0064] Compared with Example 1, Examples 7-11 only changed the incubation time. The molar ratio of the gold source solution to the serotonin solution was 1.2:1, and the remaining operating conditions and operating methods were unchanged. The incubation time used in Examples 7-11 was 30 minutes, 5 minutes, 10 minutes, 15 minutes, and 25 minutes in sequence. According to the setting of different incubation time, the absorbance of different intensity at 652nm is analyzed to get Figure 7 .

[0065] See Figure 7 It can be seen that the range of incubation time is 5-30mins. With the increase of reaction time, the UV absorption intensity and the UV absorbance change rate (c) of the system without dopamine (a) and the system with dopamine (b) are the first Increase and decrease. When the incubation time is 10mins, the three reach the maximum; the change rate of UV absorbance (c) is the lowest at 30 minutes and 5 minutes, and the system with dopamine (b) has the lowest UV absorbance at ...

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Abstract

The invention relates to the field of chemical testing and analysis and in particular relates to a method for detecting dopamine by utilizing 5-hydroxytryptamine-gold nanoparticles. The method mainly comprises preparation of the 5-hydroxytryptamine-gold nanoparticles, preparation of a 5-hydroxytryptamine-gold nanoparticle-dopamine-TMB (Tetramethylbenzidine)-H2O2 system, and detection and analysis carried out by an ultraviolet spectrophotometer. The system provided by the invention can generate a special ultraviolet absorption peak at a part with the wavelength of about 652nm, so that the dopamine is rapidly detected. According to the detection method provided by the invention, excessive complicated treatment is not carried out on gold nanoparticles and the detection method has the advantages of simplicity and convenience for operation, rapidness and short consumed time.

Description

Technical field [0001] The invention relates to the field of chemical testing and analysis, in particular to a method for detecting dopamine using serotonin-gold nanoparticles. Background technique [0002] Gold nano-particles refer to tiny gold particles with a diameter of 1-100 nm, high electron density, dielectric properties and catalytic effect, and can be combined with a variety of biological macromolecules without affecting their biological activity. Various nano-gold particles of different particle diameters can be conveniently prepared by the reduction method from chloroauric acid, and the color varies from red to purple depending on the diameter. [0003] Dopamine is a catecholamine neurotransmitter that exists in the central nervous system of mammals. Changes in its amount can cause some important diseases such as schizophrenia and Parkinson's disease. Currently, electrochemical methods are mostly used to detect dopamine, and the electrochemical methods are complicated t...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N21/33
CPCG01N21/33
Inventor 姜翠凤李朝吴辉朱佳茗朱冬芝
Owner YANCHENG INST OF TECH
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