Bacillus licheniformis Y16 and application thereof in controlling soil-borne disease of crops

A technology of Bacillus licheniformis and Y16, which is applied in the fields of microbiology and plant disease biocontrol, can solve problems such as unsatisfactory control effects, and achieve the effects of continuous yield increase, protection of farmland ecological environment, and obvious effects of promoting production and increasing yield.

Active Publication Date: 2017-06-06
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control effect is not very satisfactory, and the control effect is only about 80%. Therefore, it is very important to develop bio-fertilizers to prevent and control soil-borne diseases and continuous cropping obstacles

Method used

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  • Bacillus licheniformis Y16 and application thereof in controlling soil-borne disease of crops
  • Bacillus licheniformis Y16 and application thereof in controlling soil-borne disease of crops
  • Bacillus licheniformis Y16 and application thereof in controlling soil-borne disease of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] The preparation of embodiment 1 bacillus licheniformis Y16 microbial agent

[0036] 1. Isolation and purification of Bacillus licheniformis Y16

[0037] A strain Y16 that can effectively control sunflower sclerotinia and rust was isolated and purified from farmland soil in Gongzhuling City, Jilin Province.

[0038] According to the mycological characteristics and 16S rDNA sequencing results of the strain Y16, the strain Y16 was identified as Bacillus licheniformis.

[0039] 2. Cultivation of Bacillus licheniformis Y16

[0040] (1) Composition of medium (kg / t fermentation liquid)

[0041] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl 2.5kg, MgSO 4 ·7H 2 O 0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0042] (2) Fermentation and cultivation process

[0043] Bacillus licheniformis Y16 was inoculated on potato sucrose...

Embodiment 2

[0048] The effect of embodiment 2 Y16 bacterial agent on potted sunflower growth-promoting effect

[0049] 1. Growth-promoting effect of Y16 bacterial agent on potted sunflower in non-saline-alkaline soil

[0050] The inoculation test of Y16 fungus was carried out under greenhouse conditions. The soil came from the ancient farmland soil of Urad, Inner Mongolia. Sunflowers were planted year after year. Sunflowers were planted under potted conditions and inoculated with three kinds of biological fertilizer bacterial agents. The test results showed that all microbial agents could increase the biomass of sunflower (Table 1). The biomass of bacterial agent Y16 was the highest, increasing by 88.16% compared with the control; the bacterial agent of DSM 8785 was lower than that of Y16, increasing by 41.89% compared to the control; the biomass of the bacterial agent of DSM1732 was the lowest, increasing by 27.85% compared with the control (no microbial agent). It indicated that the Y...

Embodiment 3

[0058] Example 3 The effect of Y16 bacterial agent on the production increase of field sunflower

[0059] 1. In 2015, in Bayannaoer City, Inner Mongolia, an experimental study on the control of Sclerotinia and Puccinia with different microbial agents was carried out. The field experiment was carried out in Wulateqian Banner, Inner Mongolia. The crop was sunflower, and the variety was LDS009. The amount of microbial agent used is 50kg / mu, spread and sow the surface of the ridge row, plow 20cm, then use chemical fertilizer and sow mechanically, and cover the plastic film at the same time, the amount of nitrogen, phosphorus and potassium compound fertilizer (15:15:15) particles is 60kg / mu. The results showed that the sunflower did not have any disease during the whole growth period, neither Sclerotinia nor Puccinia disease occurred. The yield of Bacillus licheniformis Y16 was the highest, with an yield of 226.6 kg per mu, an increase of 33.33%; %; the yield of actinomycete DS...

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Abstract

The invention provides bacillus licheniformis Y16 and application thereof in controlling soil-borne disease of crops. The preservation number of the bacillus licheniformis Y16 is CGMCC NO.13512. The bacillus licheniformis Y16 has obvious growth promoting and yield increasing effects in sunflower fields when prepared into a microbial agent, the acting effect is not inhibited by field soil conditions or use of fertilizer, and the microbial agent can be applied together with fertilizer in slight alkaline farmland. The control effect of the microbial agent on head rot caused by sunflower plates achieves higher than 95%, the control effect on rust achieves 100%, the yield increase rate of sunflower is 20-30%, and the yield increase rate of greenhouse vegetables is more than 20%. The microbial agent is of great importance for control of soil-borne disease of farmland and continuous cropping obstacles, continuous increase in grain yield and protection of the ecological environment of farmland.

Description

technical field [0001] The invention relates to the technical fields of microbiology and plant disease biocontrol, in particular to a bacillus licheniformis Y16 and its application in preventing and controlling crop soil-borne diseases. Background technique [0002] With the improvement of crop yield and the development of facility agriculture, the problems of soil-borne diseases and continuous cropping obstacles have become increasingly prominent, and soil pathogenic microorganisms have continued to increase, causing crop yield reduction or even failure. Soil-borne diseases and continuous cropping obstacles have become bottlenecks in the development of safe agricultural production. Continuous cropping for 3 years will reduce yield by 10% to 20%, and crop yield will decrease by 40% to 50% in continuous cropping for 4 to 5 years. Soil-borne diseases of protected vegetables, potatoes, garlic, sunflower, rapeseed, cotton, soybean, rice, wheat, and sugar beet occur frequently, ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01P3/00A01P21/00C05G3/00C12R1/10
CPCA01N63/00C05D1/00C05G1/00C05G3/00C12N1/20C12N1/205C12R2001/10
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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