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Bacillus circulans y3 and its application in controlling soil-borne diseases of crops

A technology for bacillus circulans and soil-borne diseases, which is applied in the fields of microbiology and plant disease biocontrol, can solve problems such as unsatisfactory control effects, achieve obvious effects, increase biomass, and promote crop growth

Active Publication Date: 2020-04-10
INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The control effect is not very satisfactory, and the control effect is only about 80%. Therefore, it is very important to develop bio-fertilizers to prevent and control soil-borne diseases and continuous cropping obstacles

Method used

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  • Bacillus circulans y3 and its application in controlling soil-borne diseases of crops
  • Bacillus circulans y3 and its application in controlling soil-borne diseases of crops
  • Bacillus circulans y3 and its application in controlling soil-borne diseases of crops

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0032] Example 1 Preparation of Bacillus circulans Y3 bacterial agent

[0033] 1. Isolation and purification of Bacillus circulans Y3

[0034] A strain Y3 that can effectively control sunflower sclerotinia and rust was isolated and purified from the farmland soil of Gongzhuling, Jilin Province, my country.

[0035] According to the mycological characteristics of the strain Y3 and the 16S rDNA sequencing result (SEQ ID NO: 1), the strain Y3 was determined to be Bacillus circulans.

[0036] 2. Cultivation of Bacillus circulans Y3

[0037] (1) Composition of medium (kg / t fermentation liquid)

[0038] Soluble starch 10kg, soybean cake powder 10kg, corn flour 5kg, glucose 1kg, sucrose 10kg, yeast powder 5kg, (NH 4 ) 2 SO 4 3kg, K 2 HPO 4 0.2kg, NaCl 2.5kg, MgSO 4 ·7H 2 O 0.1kg, CaCO 3 0.5kg, FeSO 4 0.001kg, boric acid (1%) 1L, pH7.0, prepared with water.

[0039] (2) Fermentation and cultivation process

[0040] Bacillus circulans Y3 was inoculated on potato sucrose (...

Embodiment 2

[0045] Example 2 Growth-promoting effect of Bacillus circulans Y3 bacterial agent on potted corn

[0046] 1. The inoculation test of Y3 bacterial agent was carried out under greenhouse conditions, and the soil came from the farmland soil of Tangjialing. Corn was planted under pot conditions, and the biofertilizer bacterial agents prepared by the two strains were inoculated respectively. The treatments were CK, ACCC 10094 and Y3, respectively, and the strain ACCC 10094 was a potassium-solubilizing bacterium, which was used as a control strain. The results showed that both ACCC 10094 and Y3 strains had the effect of promoting crop growth, and both could increase the biomass of maize (Table 1). The fresh weight biomass of corn treated with strain ACCC10094 and Y3 reached 16.2g / pot and 18.4g / pot respectively, which were 28.6% and 46.0% higher than the control; the dry weight biomass of strain ACCC10094 and Y3 reached 1.504g / pot and 1.8626g respectively / pot, compared with the c...

Embodiment 3

[0050] The growth-promoting effect of embodiment 3 Y3 microbial agent to potted rapeseed

[0051] The potting test soil comes from Tangjialing farmland soil. Rapeseed is planted under potting conditions and inoculated with bio-fertilizer bacteria prepared by ACCC10094 and Y3 strains respectively. The fresh weight of rapeseed treated with ACCC10094 and Y3 bacterial agents reached 5.475g / pot and 7.66g / pot respectively, which were 73.3% and 142.4% higher than the control; the dry weight of rapeseed with ACCC10094 and Y3 bacterial agents was 1.036 g / pot and 1.276g / pot, respectively increased by 38.9% and 71.0% compared with the control. The bacterial agent prepared by Y3 strain significantly increased the biomass of rapeseed.

[0052] Table 2 The effect of Y3 bacterial agent on the biomass of potted rapeseed

[0053]

[0054] Note: a, b, c represent significant differences among treatment groups.

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Abstract

The invention provides a strain of Bacillus circulans Y3 and its application in preventing and controlling soil-borne diseases of crops. The preservation number CGMCC NO.13511 of the Bacillus circulans Y3, which is made into a microbial agent, has a significant control effect on sunflower soil-borne diseases sclerotinia and rust, and is effective for corn, tomato, rape, sunflower and other crops. The growth effect is obvious, and its production increase and disease prevention effect is not inhibited by the use of chemical fertilizers. Under the normal use of chemical fertilizers, the use of this bacterial agent can continue to increase production. The fungal agent has a control effect of more than 95% on sunflower rot disease caused by sclerotinia, 100% rust control effect, and a 20%-50% increase in sunflower yield. Pot test results show that it promotes crop growth and increases biomass by 15-70%. The bacterial agent is of great significance to the prevention and control of soil-borne diseases in my country's farmland, the continuous increase of grain production and the protection of the ecological environment of farmland.

Description

technical field [0001] The invention relates to the technical fields of microbiology and plant disease biocontrol, in particular to a strain of Bacillus circulans Y3 and its application in preventing and controlling crop soil-borne diseases. Background technique [0002] With the improvement of crop yield and the development of facility agriculture, the problems of soil-borne diseases and continuous cropping obstacles have become increasingly prominent, and soil pathogenic microorganisms have continued to increase, causing crop yield reduction or even failure. Soil-borne diseases and continuous cropping obstacles have become bottlenecks in the development of safe agricultural production. Continuous cropping for 3 years will reduce yield by 10% to 20%, and crop yield will decrease by 40% to 50% in continuous cropping for 4 to 5 years. Soil-borne diseases of protected vegetables, potatoes, garlic, sunflower, rapeseed, cotton, soybean, rice, wheat, and sugar beet occur frequen...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20A01N63/22A01P3/00A01P21/00C05F11/08C05G1/00C12R1/09
CPCA01N63/10C05F11/08C12N1/205C12R2001/09C05D1/00C05G1/00
Inventor 范丙全
Owner INST OF AGRI RESOURCES & REGIONAL PLANNING CHINESE ACADEMY OF AGRI SCI
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