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A bifunctional transglycosyl α-n-acetylgalactosaminidase and its expression gene and application

A technology of acetylaminogalactosidase, which is applied in the synthesis of T antigen and Tn antigen by bifunctional α-N-acetylgalactosidase, bifunctional transglycosyl α-N-acetylgalactosidase and its Gene expression and application fields can solve the problem of high price of α-N-acetylgalactosamine glycoconjugates

Active Publication Date: 2020-09-01
SHANDONG UNIV
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Due to the limitations of existing synthetic methods, the commodity price of α-N-acetylgalactosamine glycoconjugates has always been very expensive, and it is necessary to find new enzymes and cost-effective synthetic methods

Method used

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  • A bifunctional transglycosyl α-n-acetylgalactosaminidase and its expression gene and application
  • A bifunctional transglycosyl α-n-acetylgalactosaminidase and its expression gene and application
  • A bifunctional transglycosyl α-n-acetylgalactosaminidase and its expression gene and application

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Experimental program
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Embodiment Construction

[0009] A bifunctional transglycosyl α-N-acetylgalactosaminidase, the amino acid sequence is shown in SEQ ID NO.2.

[0010] The nucleotide sequence of the expression gene of the bifunctional transglycosyl α-N-acetylgalactosaminidase is shown in SEQ ID NO.1.

[0011] The application of the above-mentioned bifunctional transglycosyl α-N-acetylgalactosidase in the preparation of Tn antigen, T antigen or glycopeptide.

[0012] For the above application, the steps are as follows:

[0013] Mix the transglycosyl α-N-acetylgalactosaminidase with the glycosyl donor and the glycosyl acceptor solution to prepare a reaction system with pH 5.0 to 6.0. In the reaction system, the reaction concentration of the glycosyl donor is 5 ~10mM, the reaction concentration of glycosyl acceptor is 200~300mM, react for 10~120min at 45~55℃, stop the reaction and purify to prepare Tn and T antigen and corresponding glycopeptide;

[0014] The glycosyl donor is p-nitrobenzene-N-acetylamino-α-D-galactose or p-nitroben...

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Abstract

The invention relates to a difunctional transglycosylation alpha-N-acetamido galactosidase, an expressed gene and application thereof. The amino acid sequence is shown as SEQ ID NO.2, and the amino acid sequence of the expressed gene of difunctional transglycosylation alpha-N-acetamido galactosidase is shown as SEQ ID NO.1. According to difunctional transglycosylation alpha-N-acetamido galactosidase disclosed by the invention, Tn antigen, T antigen and corresponding glycopeptides can be efficiently synthesized, in the reaction process of synthesizing GalNAc-alpha-Ser / Thr, Gal beta 1-3GalNAc-alpha-Ser / Thr and GalNAc / Gal beta 1-3GalNAC-alpha-STAPPA glycoconjugate by using the adopted alpha-N-acetamido galactosidase in a reaction system using serine, threonine or oligopeptide (STAPPA) as receptor substrates, the yield is obviously higher than existing known related enzymes, and the difunctional transglycosylation alpha-N-acetamido galactosidase has potential application prospects.

Description

Technical field [0001] The present invention relates to a bifunctional transglycosyl α-N-acetylgalactosaminidase and its expressed gene and application, in particular to the use of a bifunctional α-N-acetyl with both endonuclease and exonuclease activities The method for synthesizing T antigen and Tn antigen and corresponding glycopeptide by galactosidase belongs to the technical field of genetic engineering. [0002] technical background [0003] α-N-Acetylgalactosaminidase (α-N-Acetylgalactosaminidase, EC 3.2.1.49) is an important type of glycoside hydrolase, which catalyzes the hydrolysis of α-N-acetylgalactosaminidase bonds and is widely distributed in bacteria In fungi, plants and animals, they belong to glycosidase families GH27, GH36, GH109 and GH129 (http: / / www.cazy.org / ) based on amino acid sequence homology. Among them, bacterial-derived α-N-acetylgalactosaminidase is distributed in GH36, GH109, ​​and GH129 families, and eukaryotic origin mainly belongs to GH27. This ty...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N9/24C12N15/56C12N15/70C12P13/06C12P13/08C12P21/00
CPCC12N9/2402C12N15/70C12N2800/101C12P13/06C12P13/08C12P21/00C12Y302/01049
Inventor 肖敏陈晓迪卢丽丽徐莉
Owner SHANDONG UNIV
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