A kind of melatonin nano composition and its preparation method and application
A nano-composition, melatonin technology, applied in the direction of drug combination, pharmaceutical formula, food science, etc., can solve the problems of easy crystallization of melatonin, low oral bioavailability, poor skin permeability, etc., to achieve excellent Skin permeability, process is easy to control, and the effect of fast skin absorption
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[0033] The present invention provides the preparation method of above-mentioned melatonin nano composition, comprises the following steps:
[0034] (1) dissolving melatonin, emulsifier, co-emulsifier and liquid lipid to obtain an oil phase;
[0035] (2) Stirring and dissolving the stabilizer, humectant and water, and filtering the obtained solution to obtain an aqueous phase;
[0036] (3) mixing the water phase obtained in the step (2) with the oil phase obtained in the step (1) while stirring, to obtain the melatonin nano composition of the present invention;
[0037] (4) The finished product is obtained after filtering the melatonin nanoemulsion obtained in the step (3);
[0038] There is no time sequence limitation between the steps (1) and (2).
[0039] The invention dissolves melatonin, emulsifier, co-emulsifier and liquid lipid to obtain oil phase. In the present invention, the temperature of the dissolution is preferably 35-80°C, more preferably 38-70°C, further pref...
Embodiment 1
[0049] Dissolve 0.1% melatonin, 1.0% Tween 60, 2.0% polyoxyethylene castor oil-20, 15.0% propylene glycol, and 1.0% isopropyl myristate in a water bath at 35°C to obtain an oil phase for later use;
[0050] Add 0.05% hydroxypropylmethylcellulose and 4.0% allantoin to 76.85% water, stir at 15 rpm for 10 min in a water bath at 35°C, filter through a 0.45 μm microporous membrane to obtain the water phase, and set aside ;
[0051] Add the water phase to the oil phase, stir in a water bath at 35° C. at a speed of 15 rpm for 10 minutes, and then filter through a 0.45 μm microporous membrane to obtain a finished melatonin nano composition.
[0052] The particle size of the melatonin nanocomposition is detected, and the particle size of the melatonin nanocomposition is 10.8nm.
Embodiment 2
[0054] 1.0% melatonin, 2.0% Tween 40, 2.0% polyoxyethylene castor oil-30, 1.0% polyethylene glycol 400 monoglyceride, 5.0% dipropylene glycol, 11.0% 1,2-hexanediol, 3.0 % polyethylene glycol, 3.0% 1,2-pentanediol, 1.0% isopropyl palmitate, 2.0% caprylic capric macrogolglyceride, dissolved in a water bath at 50°C to obtain an oil phase, set aside;
[0055] Add 0.1% polyvinylpyrrolidone, 1.0% ethyl tyrosine hydrochloride, 0.5% chitosan, and 2.0% vitamin B5 to 65.4% water, stir at 30 rpm for 15 min in a water bath at 50 ° C, and use 0.45 μm microporous membrane filtration to obtain the aqueous phase, set aside;
[0056] Add the water phase to the oil phase, stir in a water bath at 50° C. at a speed of 30 rpm for 10 min, and then filter through a 0.45 μm microporous membrane to obtain a finished melatonin nano composition.
[0057] The particle size of the melatonin nanocomposition is detected, and the particle size of the melatonin nanocomposition is 25.5nm.
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