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Kaolin-based immobilization carrier applied to methylparathion-degrading bacteria

A technology of immobilizing carrier, methyl parathion, applied in the direction of immobilization on or in inorganic carrier, inorganic chemistry, silicon compound, etc., to achieve the effect of high mechanical strength, stable property and good mass transfer performance

Inactive Publication Date: 2017-06-13
光合强化(北京)生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Immobilizing methyl parathion-degrading bacteria is one of the most effective means to restore the environment polluted by methyl parathion. However, there is still a lack of immobilized carriers for immobilizing methyl parathion-degrading bacteria. Therefore, the development of kaolin-based An immobilized carrier suitable for methyl parathion-degrading bacteria, which is of great significance for the treatment and restoration of methyl parathion-contaminated environments

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0036](1) Add 44.8 grams of kaolin to 750 mL of ammonium nitrate solution with a concentration of 8% by mass, stir at 1000 r / min for 50 minutes, filter and remove the liquid, wash with 500 mL of deionized water and place in a drying oven at 105 ° C 55 minutes, obtain substance A;

[0037] (2) Add 5.7 g of 1,4-diaminoanthraquinone to 1200 mL of absolute ethanol, stir for 5 minutes under the condition of 1000 r / min, shake well and divide into 3 equal parts to obtain mixed solution M1, mixed solution M2, Mixture M3;

[0038] (3) Add 4.8 grams of phenylhydrazine hydrochloride and 7.7 grams of 2,3,4-trimethoxybenzaldehyde into 150 mL of absolute ethanol, stir at 1000 r / min for 5 minutes, shake well and divide into 3 equal parts, Obtain mixed solution N1, mixed solution N2, mixed solution N3;

[0039] (4) Add mixed solution M1 and 8.8 g of 2-thiopheneacetyl chloride into mixed solution N1, and stir for 5 minutes at 1000 r / min to obtain mixed solution O1;

[0040] (5) Add substanc...

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Abstract

The invention discloses a kaolin-based immobilization carrier applied to methylparathion-degrading bacteria. A preparation method of the kaolin-based immobilization carrier applied to the methylparathion-degrading bacteria comprises the following steps: modifying kaolin via a mixed solution prepared from ammonium nitrate, 1,4-diamino-anthraquinone, phenylhydrazine hydrochloride, 2,3,4-trimethoxybenzaldehyde and 2-thiopheneacetyl chloride, and then preparing the kaolin into a material B; modifying the material B via a mixed solution prepared from tannic acid, pyrazoloanthrone, octamethylcyclotetrasiloxane and 2-chloro-4-fluorobenzoic acid, and then preparing the material B into a material C; modifying the material C via a mixed solution prepared from 3-amino-4-ethoxycarbonyl pyrazole, N,N-diisopropylethylamine and 3-(ethylamino)-4-methylphenol, and then preparing the material C into a material D; and modifying the material D via a mixed solution prepared from p-chlorobenzyl cyanide, benzimidazole-2-formaldehyde and acetone phenylhydrazone to obtain a material which is the kaolin-based immobilization carrier applied to the methylparathion-degrading bacteria.

Description

technical field [0001] The invention belongs to the field of research and development and preparation of functional materials for environmental pollution control technology, and in particular relates to a kaolin-based immobilized carrier suitable for methyl parathion-degrading bacteria. Background technique [0002] Immobilized microorganism technology is a biotechnology developed from immobilized enzyme technology in biochemical industry in the 1960s. By chemical or physical means, free cells are positioned in a limited space area to keep them active and can be used repeatedly. In the late 1970s, with the increasingly serious water pollution, this technology was applied to the biological treatment of industrial wastewater due to its unique advantages. Compared with the traditional suspension biological treatment method, immobilized microorganism technology has the ability to improve the microbial Cell concentration, maintaining high-efficiency strains, high microbial densit...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N11/14C02F3/34C01B33/40C02F101/38
CPCC12N11/14C01B33/40C02F3/345C02F2101/40
Inventor 不公告发明人
Owner 光合强化(北京)生物科技有限公司
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