Aflatoxin enrichment and purification method

A kind of aflatoxin, enrichment technology, applied in the direction of measuring devices, instruments, scientific instruments, etc., to achieve the effect of wide application range, good purification effect and cost saving

Active Publication Date: 2017-06-13
SHANGHAI ACAD OF AGRI SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

At present, there is still a lack of an inexpensive method that can effectively enrich and purify six aflatoxins in peanuts, corn and wheat simultaneously.

Method used

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  • Aflatoxin enrichment and purification method
  • Aflatoxin enrichment and purification method

Examples

Experimental program
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Embodiment 1

[0026] The specific steps of the toxin enrichment method are as follows:

[0027] The first step is to prepare graphene alloy nanomaterials:

[0028] (1) Take 0.5mL of chloroauric acid (0.45g / 45mL) (≥99.9%, Sigma-Aldrich Co.LLC.) and 2.5mL of L-ascorbic acid (0.4g / L) (>99%, Alfa Aisha (China) ) Chemical Co., Ltd.) Add 50mL deionized water to react at room temperature for 2min; then add 0.5mL of 1% sodium citrate solution (99%, Alfa Aisha (China) Chemical Co., Ltd.), stop the reaction after 9min; centrifuge at 13000r / min After 5 minutes, the solution was redissolved in water and ultrasonically oscillated to obtain a golden sphere solution.

[0029] (2) Dissolve 25.11 mg of graphene oxide (Nanjing Xianfeng Nano Material Technology Co., Ltd.) in 50 mL of water, adjust the pH of the solution to 10 with sodium hydroxide, add 29 g of L-cysteine ​​(Shanghai Anpu Experimental Technology Co., Ltd. Co., Ltd.), heated to 80°C and stirred for 4 hours, centrifuged at 13,000 r / min for 5 m...

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Abstract

The invention discloses an aflatoxin enrichment and purification method. The aflatoxin enrichment and purification method comprises the following steps that a graphene-alloy nanomaterial synthesized by graphene and golden balls is added into a sample extracting solution of an agricultural product to perform toxin adsorption; the sample extracting solution mixed with the nanomaterial is charged into an empty solid-phase extraction column with gaskets, and then the surface is covered with a layer of gaskets after thorough pumping; impurities are washed off with leacheate, then elution is performed by using eluant, and a concentrated solution containing a target product is obtained after the collected eluant is concentrated. The enrichment and purification method is stable and mature, can be suitable for simultaneous enrichment and purification of six types of aflatoxins in peanuts, corns, wheat and other grain and oil products and is wide in application range and good in purification effect.

Description

technical field [0001] The invention relates to the technical field of agricultural product safety detection, in particular to a method for enriching and purifying aflatoxins in agricultural products. Background technique [0002] Aflatoxins are secondary metabolites produced by Aspergillus fungi (A.flavus) and parasitic fungi (A.parasiticus), and are mostly found in agricultural products such as corn, wheat, and peanuts. Common aflatoxins include aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1), aflatoxin G2 (AFG2), aflatoxin M1 (AFM1) and aflatoxin M2 (AFM2). Aflatoxin has strong acute toxicity and chronic toxicity. Acute poisoning symptoms are vomiting, anorexia, fever, jaundice, ascites and other hepatitis symptoms; chronic toxicity mainly manifests as growth disorders such as slow growth and weight loss, subacute or chronic damage to the liver of animals, causing liver fibroblast proliferation, liver cirrhosis . In addition, aflatoxin also has strong te...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N30/08G01N30/14G01N30/06G01N30/02G01N30/72G01N30/34B82Y40/00
CPCB82Y40/00G01N30/02G01N30/06G01N30/08G01N30/14G01N30/34G01N30/72G01N2030/062G01N2030/146
Inventor 韩铮郭文博赵志辉范楷杨俊花何卫警
Owner SHANGHAI ACAD OF AGRI SCI
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